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Recent Cell Regeneration Articles

Recently published articles from Cell Regeneration.

Bone marrow-derived mesenchymal stem cell (BM-MSC): A tool of cell therapy in hydatid experimentally infected rats

December 2019
Faten A.M. Abo-Aziza | Abdel Kader A. Zaki | Amal M. Abo El-Maaty

This study aimed to clarify the potentiality of bone marrow mesenchymal stem cells (BM-MSC) transplantation with albendazole (ABZ) on the modulation of immune responses against hydatid cyst antigens...

The first cell fate decision in pre-implantation mouse embryos

December 2019
Chunmeng Yao | Wenhao Zhang | Ling Shuai

Fertilization happens when sperm and oocytes meet, which is a complicated process involving many important types of biological activation. Beginning in the 2-cell stage, an important event referred...

Efficient cleavage resolves PAM preferences of CRISPR-Cas in human cells

December 2019
Lianchao Tang | Fayu Yang | Xiaoxue He | Haihua Xie | Xiaoyu Liu | Junhao Fu | Haitao Xi | Xiaosheng Lu | Changbao Liu | Zongming Song | Jia Qu | Junzhao Zhao | Feng Gu

Clustered regularly interspaced short palindromic repeats and associated proteins (CRISPR-Cas) of bacterial adaptive immunity have been adopted as a powerful and versatile tool for manipulation of the...

Methods and applications of CRISPR/Cas system for genome editing in stem cells

December 2019
Guang Yang | Xingxu Huang

Genome editing technology holds great promise for genome manipulation and gene therapy. While widespread utilization, genome editing has been used to unravel the roles of specific genes in differentiation...

Relaunching of Cell Regeneration

December 2019
Ye-Guang Chen | Ying Lou

Retrograde monosynaptic tracing through an engineered human embryonic stem cell line reveals synaptic inputs from host neurons to grafted cells

June 2019
Qi Xing | Aiping Lin | Zhenghui Su | Chunhua Liu | Wenhao Huang | Wenjing Guo | Guangjin Pan | Yiping Guo | Xiaofen Zhong

Retrograde monosynaptic tracing with EnvA-pseudotyped rabies virus has been employed to identify the afferent and efferent connectivity of transplanted human embryonic stem (hES) cell-derived neurons...

Use of large animal models to investigate Huntington's diseases

June 2019
Sen Yan | Shihua Li | Xiao-Jiang Li

Animal models that can mimic human diseases are the important tools for investigating the pathogenesis of the diseases and finding a way for treatment. There is no doubt that small animal models have...

Nuclear-cytoplasmic shuttling of class IIa histone deacetylases regulates somatic cell reprogramming

June 2019
Zhiwei Luo | Xiaobing Qing | Christina Benda | Zhijian Huang | Meng Zhang | Yinghua Huang | Hui Zhang | Lulu Wang | Yiwei Lai | Carl Ward | Giacomo Volpe | Xiaofen Zhong | Baoming Qin | Qiang Zhuang | Miguel A. Esteban | Wenjuan Li

Class IIa histone deacetylases (HDACs) are a subfamily of HDACs with important functions in development and adult tissue homeostasis. As opposed to other HDACs, they lack catalytic function and bind...

Molecular switch model for cardiomyocyte proliferation

June 2019
Satwat Hashmi | H.R. Ahmad

This review deals with the human adult cardiomyocyte proliferation as a potential source for heart repair after injury. The mechanism to regain the proliferative capacity of adult cardiomyocytes is...

Research advances in erythrocyte regeneration sources and methods in vitro

December 2018
Shuming Sun | Yuanliang Peng | Jing Liu

Erythrocytes (red blood cells, RBCs) facilitate gas exchange in the lungs and transport oxygen to the tissues. The human body must maintain erythrocyte regeneration to support metabolically active cells...

Approaches for generation of anti-leukemia specific T cells

December 2018
Zhenyi Jin | Ling Xu | Yangqiu Li

As three decades ago, it was reported that adoptive T cell immunotherapy by infusion of autologous tumor infiltrating lymphocytes (TILs) mediated objective cancer regression in patients with metastatic...

Illumination of neural development by in vivo clonal analysis

December 2018
Mingrui Xu | Jingjing Wang | Xize Guo | Tingting Li | Xia Kuang | Qing-Feng Wu

Single embryonic and adult neural stem cells (NSCs) are characterized by their self-renewal and differentiation potential. Lineage tracing via clonal analysis allows for specific labeling of a single...

Long non-coding RNAs in hematopoietic regulation

December 2018
Weiqian Li | Yue Ren | Yanmin Si | Fang Wang | Jia Yu

Long non-coding RNAs (lncRNAs) have crucial roles via tethering with DNA, RNA or protein in diverse biological processes. These lncRNA-mediated interactions enhance gene regulatory networks and modulate...

Hematopoiesis and microenvironment in hematological malignancies

September 2018
Hui Cheng | Guohuan Sun | Tao Cheng

Adult hematopoietic stem cells (HSCs) and progenitors (HPCs) reside in the bone marrow, a highly orchestrated architecture. In the bone marrow, the process of how HSCs exert self-renewal and differentiation...

A protocol for generating induced T cells by reprogramming B cells in vivo

September 2018
Qitong Weng | Fangxiao Hu | Mengyun Zhang | Yong Dong | Cui Lv | Ying Wang | Xiaofei Liu | Jinyong Wang

Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This...

Mouse embryonic stem cells resist c-Jun induced differentiation when in suspension

September 2018
Bo Wang | Dongwei Li | Jiekai Chen | Jing Liu | Duanqing Pei

The oncogene c-Jun plays a key role in development and cancer. Yet, its role in cell fate decision remains poorly understood at the molecular level. Here we report that c-Jun confers different fate...

Cardiac progenitor reprogramming for heart regeneration

September 2018
Behnam Ebrahimi

Myocardial infarction leads to the loss of a huge number of cardiomyocytes and the reparatory response to this phenomenon is scar tissue formation, which impairs heart function. Direct reprogramming...

Genomic and molecular control of cell type and cell type conversions

December 2017
Xiuling Fu | Fangfang He | Yuhao Li | Allahverdi Shahveranov | Andrew Paul Hutchins

Organisms are made of a limited number of cell types that combine to form higher order tissues and organs. Cell types have traditionally been defined by their morphologies or biological activity, yet...

RNA-binding proteins in mouse male germline stem cells: a mammalian perspective

Huayu Qi

Adult stem cells that reside in particular types of tissues are responsible for tissue homeostasis and regeneration. Cellular functions of adult stem cells are intricately related to the gene expression...

Dissecting microRNA-mediated regulation of stemness, reprogramming, and pluripotency

Young Jin Lee | Suresh Ramakrishna | Himanshu Chauhan | Won Sun Park | Seok-Ho Hong | Kye-Seong Kim

Increasing evidence indicates that microRNAs (miRNAs), endogenous short non-coding RNAs 19–24 nucleotides in length, play key regulatory roles in various biological events at the post-transcriptional...

Loss of Mgat5a-mediated N-glycosylation stimulates regeneration in zebrafish

Wuhong Pei | Sunny C. Huang | Lisha Xu | Kade Pettie | María Laura Ceci | Mario Sánchez | Miguel L. Allende | Shawn M. Burgess

We are using genetics to identify genes specifically involved in hearing regeneration. In a large-scale genetic screening, we identified mgat5a, a gene in the N-glycosylation biosynthesis pathway whose...

ETV2 expression increases the efficiency of primitive endothelial cell derivation from human embryonic stem cells

Anne G Lindgren | Matthew B Veldman | Shuo Lin

Endothelial cells line the luminal surface of blood vessels and form a barrier between the blood and other tissues of the body. Ets variant 2 (ETV2) is transiently expressed in both zebrafish and mice...

Pharmacological manipulation of transcription factor protein-protein interactions: opportunities and obstacles

Frank Fontaine | Jeroen Overman | Mathias François

Much research on transcription factor biology and their genetic pathways has been undertaken over the last 30 years, especially in the field of developmental biology and cancer. Yet, very little is...

Wnt/β-catenin signaling in heart regeneration

Gunes Ozhan | Gilbert Weidinger

The ability to repair damaged or lost tissues varies significantly among vertebrates. The regenerative ability of the heart is clinically very relevant, because adult teleost fish and amphibians can...

GATA2−/− human ESCs undergo attenuated endothelial to hematopoietic transition and thereafter granulocyte commitment

Ke Huang | Juan Du | Ning Ma | Jiajun Liu | Pengfei Wu | Xiaoya Dong | Minghui Meng | Wenqian Wang | Xin Chen | Xi Shi | Qianyu Chen | Zhongzhou Yang | Shubin Chen | Jian Zhang | Yuhang Li | Wei Li | Yi Zheng | Jinglei Cai | Peng Li | Xiaofang Sun | Jinyong Wang | Duanqing Pei | Guangjin Pan

Hematopoiesis is a progressive process collectively controlled by an elaborate network of transcription factors (TFs). Among these TFs, GATA2 has been implicated to be critical for regulating multiple...

The interplay between metabolic homeostasis and neurodegeneration: insights into the neurometabolic nature of amyotrophic lateral sclerosis

S.T. Ngo | F.J. Steyn

Amyotrophic lateral sclerosis (ALS) is a fatal, neurodegenerative disease that is characterized by the selective degeneration of upper motor neurons and lower spinal motor neurons, resulting in the...

Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art

Eleni Oberbauer | Carolin Steffenhagen | Christoph Wurzer | Christian Gabriel | Heinz Redl | Susanne Wolbank

In the past decade, adipose tissue became a highly interesting source of adult stem cells for plastic surgery and regenerative medicine. The isolated stromal vascular fraction (SVF) is a heterogeneous...

Stem cell aging in adult progeria

Hoi-Hung Cheung | Duanqing Pei | Wai-Yee Chan

Aging is considered an irreversible biological process and also a major risk factor for a spectrum of geriatric diseases. Advanced age-related decline in physiological functions, such as neurodegeneration,...

Regulatory functions and pathological relevance of the MECP2 3′UTR in the central nervous system

Heather McGowan | Zhiping P. Pang

Methyl-CpG-binding protein 2 (MeCP2), encoded by the gene MECP2, is a transcriptional regulator and chromatin-remodeling protein, which is ubiquitously expressed and plays an essential role in the development...

Reprogramming barriers and enhancers: strategies to enhance the efficiency and kinetics of induced pluripotency

Behnam Ebrahimi

Induced pluripotent stem cells are powerful tools for disease modeling, drug screening, and cell transplantation therapies. These cells can be generated directly from somatic cells by ectopic expression...

Vascularization mediated by mesenchymal stem cells from bone marrow and adipose tissue: a comparison

Karoline Pill | Sandra Hofmann | Heinz Redl | Wolfgang Holnthoner

Tissue-engineered constructs are promising to overcome shortage of organ donors and to reconstruct at least parts of injured or diseased tissues or organs. However, oxygen and nutrient supply are limiting...

Stem cell therapy for amyotrophic lateral sclerosis

Zhijuan Mao | Suming Zhang | Hong Chen

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the loss of motor neurons. Currently, no effective therapy is available to treat ALS, except for Riluzole,...

Reprogramming somatic cells to cells with neuronal characteristics by defined medium both in vitro and in vivo

Songwei He | Yiping Guo | Yixin Zhang | Yuan Li | Chengqian Feng | Xiang Li | Lilong Lin | Lin Guo | Haitao Wang | Chunhua Liu | Yi Zheng | Chuanming Luo | Qiang Liu | Fuhui Wang | Hao Sun | Lining Liang | Lingyu Li | Huanxing Su | Jiekai Chen | Duanqing Pei | Hui Zheng

Currently, direct conversion from somatic cells to neurons requires virus-mediated delivery of at least one transcriptional factor or a combination of several small-molecule compounds. Delivery of transcriptional...

Characterization of a novel cell penetrating peptide derived from human Oct4

Eva Harreither | Hanna A Rydberg | Helene L Åmand | Vaibhav Jadhav | Lukas Fliedl | Christina Benda | Miguel A Esteban | Duanqing Pei | Nicole Borth | Regina Grillari-Voglauer | Oliver Hommerding | Frank Edenhofer | Bengt Nordén | Johannes Grillari

Oct4 is a transcription factor that plays a major role for the preservation of the pluripotent state in embryonic stem cells as well as for efficient reprogramming of somatic cells to induced pluripotent...

Generation of knockout rabbits using transcription activator-like effector nucleases

Yu Wang | Nana Fan | Jun Song | Juan Zhong | Xiaogang Guo | Weihua Tian | Quanjun Zhang | Fenggong Cui | Li Li | Philip N Newsome | Jon Frampton | Miguel A Esteban | Liangxue Lai

Zinc-finger nucleases and transcription activator-like effector nucleases are novel gene-editing platforms contributing to redefine the boundaries of modern biological research. They are composed of...

glbase: a framework for combining, analyzing and displaying heterogeneous genomic and high-throughput sequencing data

Andrew Paul Hutchins | Ralf Jauch | Mateusz Dyla | Diego Miranda-Saavedra

Genomic datasets and the tools to analyze them have proliferated at an astonishing rate. However, such tools are often poorly integrated with each other: each program typically produces its own custom...

OCT4: A penetrant pluripotency inducer

Xuecong Wang | Ralf Jauch

Native OCT4 protein has the intrinsic ability of crossing cellular membranes to enter cells. This finding could revive efforts to induce pluripotency with proteins replacing nucleic acid-based approaches,...

Role of Oct4 in the early embryo development

Guangming Wu | Hans R Schöler

Oct4 is a key component of the pluripotency regulatory network, and its reciprocal interaction with Cdx2 has been shown to be a determinant of either the self-renewal of embryonic stem cells (ESCs)...

Bioengineering of a human whole tooth: progress and challenge

Yanding Zhang | YiPing Chen

A major challenge in stem cell-based bioengineering of an implantable human tooth is to identify appropriate sources of postnatal stem cells that are odontogenic competent as the epithelial component...

Genetic reporter analysis reveals an expandable reservoir of OCT4+ cells in adult skin

Anne Limbourg | Sabine Schnabel | Vladimir J Lozanovski | L Christian Napp | Teng-Cheong Ha | Tobias Maetzig | Johann Bauersachs | Hassan Y Naim | Axel Schambach | Florian P Limbourg

The transcription factor Oct4 (Pou5f1) is a critical regulator of pluripotency in embryonic and induced pluripotent stem cells. Therefore, Oct4 expression might identify somatic stem cell populations...

Generation of multi-gene knockout rabbits using the Cas9/gRNA system

Quanmei Yan | Quanjun Zhang | Huaqiang Yang | Qingjian Zou | Chengcheng Tang | Nana Fan | Liangxue Lai

The prokaryotic clustered regularly interspaced short palindromic repeat (CRISPR)-associated system (Cas) is a simple, robust and efficient technique for gene targeting in model organisms such as zebrafish,...

Production of transgenic pigs over-expressing the antiviral gene Mx1

Quanmei Yan | Huaqiang Yang | Dongshan Yang | Bentian Zhao | Zhen Ouyang | Zhaoming Liu | Nana Fan | Hongsheng Ouyang | Weiwang Gu | Liangxue Lai

The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate gene to improve disease resistance in farm animals. In this study, we report...

Exploring the utility of organo-polyoxometalate hybrids to inhibit SOX transcription factors

Kamesh Narasimhan | Kevin Micoine | Emmanuel Lacôte | Serge Thorimbert | Edwin Cheung | Bernold Hasenknopf | Ralf Jauch

SOX transcription factors constitute an attractive target class for intervention with small molecules as they play a prominent role in the field of regenerative biomedicine and cancer biology. However,...

Combined influence of basal media and fibroblast growth factor on the expansion and differentiation capabilities of adipose-derived stem cells

Mark Ahearne | Joanne Lysaght | Amy P Lynch

Interest in adipose-derived stem cells (ASCs) has increased in recent years due to their multi-linage differentiation capabilities. While much work has been done to optimize the differentiation media,...

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