Defense-associated reverse transcriptases define an emerging class of noncanonical DNA-writing systems
December 2026
Defense-associated reverse transcriptases (DRTs) are emerging as a mechanistically diverse class of bacterial antiviral enzymes. Rather than acting simply as RNA-to-DNA copying proteins, they generate...
Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering
September 2025
Astaxanthin is a natural red carotenoid, commonly used as an additive in the pharmaceutical industry and as a nutritional supplement owing to its notable antioxidant benefits. However, a complex biosynthetic...
Eukaryotic cell-free protein synthesis: Chassis diversification, system engineering, and emerging applications
December 2026
The eukaryotic cell-free protein synthesis (CFPS) system, endowed with intrinsic post-translational modification capabilities and a complex molecular chaperone network, efficiently synthesizes functional...
Engineering Rubisco to enhance CO2 utilization
March 2024
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a pivotal enzyme that mediates the fixation of CO2. As the most abundant protein on earth, Rubisco has a significant impact on global carbon,...
Multi-gene Co-expression systems in E. coli: From single-vector designs to programmable expression platforms
June 2026
Escherichia coli (E. coli) has long served as a versatile workhorse for recombinant protein production. As synthetic biology expands the demand for coordinated expression of multiple genes, co-expression...
OmniEdit: A unified CRISPR/Cas9 platform for precise genome engineering and strain optimization of the Cordyceps militaris cell factory
December 2026
Cordyceps militaris serves as a critical microbial cell factory for high-value bioactive compounds; however, the scarcity of versatile and sophisticated genome-editing toolsets significantly restricts...
Development of an efficient genome editing system using recombinase-mediated cassette exchange in E. coli Nissle 1917 for large gene cluster integration and heterologous astaxanthin production
December 2026
Escherichia coli Nissle 1917 (EcN) is a promising chassis strain in synthetic biology, but its application is limited by inefficient genetic manipulation. This study established an efficient genetic...
Enhanced production of l-histidine in Escherichia coli through systematic metabolic engineering and CER controlled fermentation
December 2026
l-Histidine is an essential amino acid with important applications in pharmaceuticals and nutrition, highlighting the demand for efficient microbial production platforms. This study developed a high-performance...
DNA storage: The future direction for medical cold data storage
June 2025
DNA storage, characterized by its durability, data density, and cost-effectiveness, is a promising solution for managing the increasing data volumes in healthcare....
Profiling heterologous 3′ terminator-dependent expression variation in Aspergillus oryzae
December 2026
Transcriptional terminators are key to defining transcript boundaries, ensuring mRNA maturation, and maintaining expression stability, yet they remain underexplored in filamentous fungi. Here, we systematically...
Engineering genetic elements for microbial protein expression systems: Advances, challenges, applications, and prospects
March 2026
The rising global demand for sustainable protein sources poses critical challenges across food, pharmaceutical, and industrial biotechnology sectors. Microbial expression systems provide scalable and...
Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production
June 2025
Corynebacterium glutamicum is a versatile industrial microorganism for producing various amino acids. However, there have been no reports of well-defined C. glutamicum strains capable of hyperproducing...
High-level production of vitamin K2 in Escherichia coli via modular molecular engineering
June 2026
Menaquinone-7 (MK-7), a key form of vitamin K2 with wide-ranging nutritional and pharmaceutical applications, has attracted increasing interest for microbial production. Here, we developed an integrated...
Efficient expression of full-length triple-helix recombinant type III collagen in Pichia pastoris
January 2027
Type III collagen (COL3A1) is a significant fibrillar collagen abundant in vascular and cutaneous tissues, playing vital roles in wound healing and tissue repair. The growing demand for COL3A1 in biomedical...
Advances in engineering and applications of synthetic phase-separated membraneless organelles in biotechnology
September 2026
Membraneless organelles (MLOs) formed through liquid-liquid phase separation (LLPS) constitute crucial dynamic microenvironments within cells, capable of selectively concentrating specific molecules...
Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach
September 2026
Peptide linkers are critical modulators of function in fusion proteins, a foundational technology in modern bioengineering. However, the rational customization of linkers for specific applications remains...
Synergistic engineering PETase reveals loop-region mutations for enhanced catalytic activity and thermal stability
March 2026
Biodegradation of Polyethylene Terephthalate (PET) offer a strategic avenue for addressing the global plastic pollution crisis. However, the degradation performance of PET hydrolases remains a technological...
Ergothioneine biosynthesis: The present state and future prospect
March 2025
Ergothioneine (ERG), a rare natural thio-histidine derivative with potent antioxidant properties and diverse biological functions, is widely utilized in food processing, cosmetics, pharmaceuticals,...
Engineering a heme-dependent tryptophan hydroxylase pathway in E. coli for enhanced melatonin production
December 2026
Melatonin is a high-value bioactive indoleamine broadly applied in the pharmaceutical, food and nutraceutical industries, yet its microbial production remains constrained by pathway complexity and low...
The PreKit platform: Cryptic gene clusters activation and high-titer compound production
December 2026
The discovery of novel scaffold compounds or enzymes through genome mining of biosynthetic gene clusters (BGCs) represents cornerstone of drug development. A major obstacle, however, is that most microbial...
Metabolic engineering and adaptive laboratory evolution enhance squalene production in Yarrowia lipolytica
September 2026
Squalene, a multifunctional natural compound with diverse bioactivities, has significant potential in the nutraceutical and health industries. Microbial synthesis using engineered cell factories represents...
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds
June 2022
Per- and Polyfluorinated alkyl substances (PFAS) are a broad class of synthetic compounds that have fluorine substituted for hydrogen in several or all locations and are globally categorized as PFCs...
Development of mycelium-reinforced dialdehyde cellulose–polyvinyl alcohol composites for emerging high-performance sustainable materials
September 2026
Dialdehyde cellulose (DAC), featuring reactive carbonyl groups introduced via oxidation of hemp-derived cellulose fibers (confirmed by FTIR spectroscopy), was synthesized to promote strong hydrogen...
A systematic discussion and comparison of the construction methods of synthetic microbial community
December 2024
Synthetic microbial community has widely concerned in the fields of agriculture, food and environment over the past few years. However, there is little consensus on the method to synthetic microbial...
Synergistic engineering of monoterpenoid synthase and metabolic pathways to enhance α-terpineol production in Saccharomyces cerevisiae
December 2026
Plant-derived monoterpenoid α-terpineol has gained significant attention due to its versatile applications in the food, cosmetic and pharmaceutical industries. Microbial biosynthesis offers an environmentally...