Enhancing 3-hydroxypropionic acid production from recombinant Saccharomyces cerevisiae for using rice straw hydrolysate and sugar cane industrial waste as substrate
December 2025
Rice straw and sugar cane industrial waste are a plentiful source of lignocellulosic biomass with a high polysaccharide content, that is hydrolyzed into sugar for microbial growth and their metabolites....
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Improving fidaxomicin production through ARTP mutagenesis and fermentation optimization in Actinoplanesdeccanensis
December 2025
Fidaxomicin, a macrolide antibiotic, is widely used to treat Clostridioides difficile infection (CDI). It demonstrats significantly higher clinical efficacy than vancomycin and metronidazole. However,...
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Transcriptomic studies on the product stress response revealed that YCF1 is a beneficial factor for progesterone production in Yarrowia lipolytica
December 2025
Progesterone is a widely used therapeutic hormone and a common precursor for the synthesis of pharmaceutical steroids in both mammals and plants. It has been successfully produced in heterologous microorganisms,...
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Metabolic engineering of Escherichia coli for squalene overproduction
December 2025
Squalene, a lipophilic triterpene with multifaceted bioactivities, faces bioproduction bottlenecks in microbial hosts due to inefficient biosynthetic pathways and limited storage capacity. Here, we...
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Systematic design and evaluation of artificial CO2 assimilation pathways
December 2025
With the advancement of industry and bio-agriculture, the effective management of CO2 has emerged as a critical challenge for humanity. This study systematically explores multiple CO2 assimilation pathways...
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Recombinant production of amaranthin and other betalain variants with yeast cell factories
December 2025
Betalains are a class of natural pigments found in plants of the Caryophyllales order. Betanin is the dominant betalain on the food colour market, even though over 80 other variants are known. Recombinant...
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Decoding yeast transcriptional regulation via a data-and mechanism-driven distributed large-scale network model
December 2025
The complex transcriptional regulatory relationships among genes influence gene expression levels and play a crucial role in determining cellular phenotypes. In this study, we propose a novel, distributed,...
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Engineering T7 RNA polymerase-cascaded systems controlled by nisin and theophylline for protein overexpression and targeted gene mutagenesis in Lactococcus lactis
December 2025
Lactococcus lactis serves as an important platform for heterologous protein production, with the nisin-controlled gene expression (NICE) system being widely employed for regulated protein overexpression....
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Genomic and transcriptomic insights into the molecular responses of a biocrust-derived oleaginous microalga Vischeria sp. WL1 to nitrogen depletion and recovery
December 2025
Microalgae surviving in extreme conditions evolve tolerance mechanisms to diverse environmental stresses. Environmental nitrogen limitation or nitrogen starvation poses a significant threat to the growth...
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Engineering Yarrowia lipolytica for high-level β-elemene production via combinatorial metabolic strategies
December 2025
β-Elemene, a pharmacologically active sesquiterpene derived from the traditional Chinese medicinal herb Curcuma wenyujin, exhibits broad-spectrum anti-tumor and anti-inflammatory activities. Currently,...
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Enhanced chlorogenic acid production from glucose via systematic metabolic engineering of Saccharomyces cerevisiae
September 2025
Chlorogenic acid (CGA) is a valuable phenolic acid with various pharmaceutical functions. In our previous study, de novo synthesis of CGA in Saccharomyces cerevisiae was achieved. However, its yield...
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Combined metabolic and enzymatic engineering for de novo biosynthesis of δ-tocotrienol in Yarrowia lipolytica
September 2025
δ-Tocotrienol, an isomer of vitamin E with anti-inflammatory, neuroprotective and anti-coronary arteriosclerosis properties, is widely used in health care, medicine and other fields. Microbial synthesis...
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Optimized dual-AAV base editor delivery system with enhanced editing efficiency and virion production titer
September 2025
Base editors (BEs) are a promising tool for precise base conversion in human cells and animals, while the adeno-associated virus (AAV) is the major vector for human gene therapy. However, the size of...
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iCASRED, a scarless DNA editing tool in E. coli for high-efficiency engineering of natural product biosynthetic gene clusters
September 2025
Efficient gene cluster editing tools are one of the key techniques for discovering novel compounds encoded by silent natural product (NP) biosynthetic gene clusters (BGCs) in microbial genomes. Currently,...
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Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering
September 2025
Astaxanthin is a natural red carotenoid, commonly used as an additive in the pharmaceutical industry and as a nutritional supplement owing to its notable antioxidant benefits. However, a complex biosynthetic...
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Identification of two new flavone 4′-O-methyltransferases and their application in de novo biosynthesis of (2S)-hesperetin in Yarrowia lipolytica
September 2025
Methyltransferases are pivotal enzymes in the biosynthesis of methylated flavonoids, including (2S)-hesperetin. However, existing flavonoid 4′-O-methyltransferase (F4′OMT) enzymes typically exhibit...
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PEX3 gene knockout influences recombinant xylanase expression by Komagataella phaffii
September 2025
Komagataella phaffii is a methylotrophic yeast harboring a tightly regulated alcohol oxidase promoter (PAOX1), which is now widely used for recombinant protein production. During PAOX1 expression phase...
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Development of ultrasound-visualized tumor-targeting engineered bacteria for precise tumor therapy
September 2025
In situ imaging diagnosis and precise treatment of deep tumor tissues are hotspots in life sciences and medical research. In recent years, using focused ultrasound to remotely control engineered bacteria...
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MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila
September 2025
Nowadays, biological databases are playing an increasingly critical role in biological research. Myceliophthora thermophila is an excellent thermophilic fungal chassis for industrial enzyme production...
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A new paradigm for the regulation of A40926B0 biosynthesis
September 2025
Dalbavancin is a novel glycopeptide antibiotic with activity against a broad range of Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). A40926B0 is the direct precursor...
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Efficient production of 2′-fucosyllactose in Pichia pastoris through metabolic engineering and constructing an orthogonal energy supply system
September 2025
2′-fucosyllactose (2′-FL) holds significant role in the infants' nutrition. While microbial production of 2′-FL has predominantly utilized Escherichia coli and Saccharomyces cerevisiae, the potential...
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A systematic study of regulating inorganic polyphosphates production in Saccharomyces cerevisiae
September 2025
Inorganic polyphosphate (polyP), a linear polymer of orthophosphate residues, plays critical roles in diverse biological processes spanning blood coagulation, immunomodulation, and post-translational...
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Programming anti-ribozymes to sense trigger RNAs for modulating gene expression in mammalian cells
September 2025
Synthetic RNA-based switches provide distinctive merits in modulating gene expression. Simple and flexible RNA-based switches are crucial for advancing the field of gene regulation, paving the way for...
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Enhanced l-serine synthesis in Corynebacterium glutamicum by exporter engineering and Bayesian optimization of the medium composition
September 2025
l-serine is a versatile, high value-added amino acid, widely used in food, medicine and cosmetics. However, the low titer of l-serine has limited its industrial production. In this study, a cell factory...
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Nonglycosidic C–O bond formation catalyzed by a bifunctional pseudoglycosyltransferase ValL
September 2025
The C7N antibiotic validamycin A is an antifungal agent widely used as a crop protectant. It comprises a validoxylamine A unit linked to a glucose moiety, which is formed through a nonglycosidic C–N...
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