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Most Downloaded Synthetic and Systems Biotechnology Articles

The most downloaded articles from ScienceDirect in the last 90 days.

1. CRISPR-Cas9/Cas12a biotechnology and application in bacteria

September 2018
Ruilian Yao | Di Liu | Xiao Jia | Yuan Zheng | Wei Liu | Yi Xiao

CRISPR-Cas technologies have greatly reshaped the biology field. In this review, we discuss the CRISPR-Cas with a particular focus on the associated technologies and applications of CRISPR-Cas9 and...

2. Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast

March 2020
Tadas Jakočiūnas | Andreas K. Klitgaard | Eftychia Eva Kontou | Julie Bang Nielsen | Emil Thomsen | David Romero-Suarez | Kai Blin | Christopher J. Petzold | Jennifer W. Gin | Yaojun Tong | Charlotte Held Gotfredsen | Pep Charusanti | Rasmus J.N. Frandsen | Tilmann Weber | Sang Yup Lee | Michael K. Jensen | Jay D. Keasling

To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new,...

3. Engineering and modification of microbial chassis for systems and synthetic biology

March 2019
Haotian Chi | Xiaoli Wang | Yue Shao | Ying Qin | Zixin Deng | Lianrong Wang | Shi Chen

Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health, medicine, agricultural,...

4. Cell-free synthetic biology: Engineering in an open world

March 2017
Yuan Lu

Cell-free synthetic biology emerges as a powerful and flexible enabling technology that can engineer biological parts and systems for life science applications without using living cells. It provides...

5. Reactive oxygen species and antioxidant properties from mushrooms

March 2017
Carmen Sánchez

Preventive medicine and food industry have shown an increased interest in the development of natural antioxidants, since those most commonly used synthetic antioxidants may have restricted use in food....

6. A review of computational tools for design and reconstruction of metabolic pathways

December 2017
Lin Wang | Satyakam Dash | Chiam Yu Ng | Costas D. Maranas

Metabolic pathways reflect an organism's chemical repertoire and hence their elucidation and design have been a primary goal in metabolic engineering. Various computational methods have been developed...

7. A living foundry for Synthetic Biological Materials: A synthetic biology roadmap to new advanced materials

June 2018
Rosalind A. Le Feuvre | Nigel S. Scrutton

Society is on the cusp of harnessing recent advances in synthetic biology to discover new bio-based products and routes to their affordable and sustainable manufacture. This is no more evident than...

8. Biofilm architecture: An emerging synthetic biology target

March 2020
Stephen J. Kassinger | Monique L. van Hoek

Synthetic biologists are exploiting biofilms as an effective mechanism for producing various outputs. Metabolic optimization has become commonplace as a method of maximizing system output. In addition...

9. Fundamental CRISPR-Cas9 tools and current applications in microbial systems

September 2017
Pingfang Tian | Jia Wang | Xiaolin Shen | Justin Forrest Rey | Qipeng Yuan | Yajun Yan

Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we...

10. Activation of microbial secondary metabolic pathways: Avenues and challenges

September 2018
Bikash Baral | Amir Akhgari | Mikko Metsä-Ketelä

Microbial natural products are a tremendous source of new bioactive chemical entities for drug discovery. Next generation sequencing has revealed an unprecedented genomic potential for production of...

11. The secondary metabolite bioinformatics portal: Computational tools to facilitate synthetic biology of secondary metabolite production

June 2016
Tilmann Weber | Hyun Uk Kim

Natural products are among the most important sources of lead molecules for drug discovery. With the development of affordable whole-genome sequencing technologies and other ‘omics tools, the field...

12. Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001

September 2019
Sagarika Devi | Heiko T. Kiesewalter | Renátó Kovács | Jens Christian Frisvad | Tilmann Weber | Thomas Ostenfeld Larsen | Ákos T. Kovács | Ling Ding

For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced...

13. Transcription control engineering and applications in synthetic biology

September 2017
Michael D. Engstrom | Brian F. Pfleger

In synthetic biology, researchers assemble biological components in new ways to produce systems with practical applications. One of these practical applications is control of the flow of genetic information...

14. A brief overview of synthetic biology research programs and roadmap studies in the United States

December 2016
Tong Si | Huimin Zhao

The United States is a leading nation in the development of synthetic biology, an emerging engineering discipline to create, control and reprogram biological systems. With strategic investment from...

15. Benchmarking two Saccharomyces cerevisiae laboratory strains for growth and transcriptional response to methanol

December 2019
Monica I. Espinosa | Thomas C. Williams | Isak S. Pretorius | Ian T. Paulsen

One-carbon compounds, such as methanol, are becoming potential alternatives to sugars as feedstocks for the biological production of chemicals, fuels, foods, and pharmaceuticals. Efficient biological...

16. Engineering bacteria for enhanced polyhydroxyalkanoates (PHA) biosynthesis

September 2017
Guo-Qiang Chen | Xiao-Ran Jiang

Polyhydroxyalkanoates (PHA) have been produced by some bacteria as bioplastics for many years. Yet their commercialization is still on the way. A few issues are related to the difficulty of PHA commercialization:...

17. Simplified methodology for a modular and genetically expanded protein synthesis in cell-free systems

December 2019
Yonatan Chemla | Eden Ozer | Michael Shaferman | Ben Zaad | Rambabu Dandela | Lital Alfonta

Genetic code expansion, which enables the site-specific incorporation of unnatural amino acids into proteins, has emerged as a new and powerful tool for protein engineering. Currently, it is mainly...

18. Streamlining the preparation of “endotoxin-free” ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase

December 2019
J. Porter Hunt | Emily Long Zhao | Mehran Soltani | Madison Frei | J. Andrew D. Nelson | Bradley C. Bundy

An “endotoxin-free” E. coli-based cell-free protein synthesis system has been reported to produce therapeutic proteins rapidly and on-demand. However, preparation of the most complex CFPS reagent –...

19. Blossom of CRISPR technologies and applications in disease treatment

December 2018
Huayi Liu | Lian Wang | Yunzi Luo

Since 2013, the CRISPR-based bacterial antiviral defense systems have revolutionized the genome editing field. In addition to genome editing, CRISPR has been developed as a variety of tools for gene...

20. Prospective study of probiotic supplementation results in immune stimulation and improvement of upper respiratory infection rate

June 2018
Hong Zhang | Chiajung Yeh | Zonglian Jin | Liwei Ding | Bryan Y. Liu | Li Zhang | H. Kathleen Dannelly

The human gut microbiota is an important environmental factor for human health with evolutionarily conserved roles in immunity, metabolism, development, and behavior of the host. Probiotic organisms...

21. Methods to reduce variability in E. Coli-based cell-free protein expression experiments

December 2019
Jared L. Dopp | Yeong Ran Jo | Nigel F. Reuel

Cell-free protein synthesis (CFPS) is an established biotechnology tool that has shown great utility in many applications such as prototyping proteins, building genetic circuits, designing biosensors,...

22. Phosphate limitation increases coenzyme Q10 production in industrial Rhodobacter sphaeroides HY01

December 2019
Lu Zhang | Leshi Liu | Ke-Feng Wang | Lan Xu | Liming Zhou | Weishan Wang | Chuan Li | Zheng Xu | Tong Shi | Haihong Chen | Yuanhang Li | Hui Xu | XiuLiang Yang | Zhichun Zhu | Biqin Chen | Dan Li | Guanghuang Zhan | Si-Liang Zhang | Li-Xin Zhang | Gao-Yi Tan

Coenzyme Q10 (CoQ10) is an important component of the respiratory chain in humans and some bacteria. As a high-value-added nutraceutical antioxidant, CoQ10 has excellent capacity to prevent cardiovascular...

23. Therapeutic applications of genetic code expansion

September 2018
Yujia Huang | Tao Liu

In nature, a limited, conservative set of amino acids are utilized to synthesize proteins. Genetic code expansion technique reassigns codons and incorporates noncanonical amino acids (ncAAs) through...

24. Development of fungal cell factories for the production of secondary metabolites: Linking genomics and metabolism

March 2017
Jens Christian Nielsen | Jens Nielsen

The genomic era has revolutionized research on secondary metabolites and bioinformatics methods have in recent years revived the antibiotic discovery process after decades with only few new active molecules...

25. Simple glycolipids of microbes: Chemistry, biological activity and metabolic engineering

March 2018
Ahmad Mohammad Abdel-Mawgoud | Gregory Stephanopoulos

Glycosylated lipids (GLs) are added-value lipid derivatives of great potential. Besides their interesting surface activities that qualify many of them to act as excellent ecological detergents, they...

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