DIProT: A deep learning based interactive toolkit for efficient and effective Protein design
June 2024
The protein inverse folding problem, designing amino acid sequences that fold into desired protein structures, is a critical challenge in biological sciences. Despite numerous data-driven and knowledge-driven...
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A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds
June 2024
The goal of this study was to use statistical optimization to change the nutritional and environmental conditions so that Streptomyces baarensis MH-133 could make more active metabolites. Twelve trials...
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Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology
June 2024
Bacteria exhibit a rich repertoire of RNA molecules that intricately regulate gene expression at multiple hierarchical levels, including small RNAs (sRNAs), riboswitches, and antisense RNAs. Notably,...
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A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger
June 2024
Aspergillus niger is a highly versatile fungal strain utilized in industrial production. The expression levels of recombinant genes in A. niger can be enhanced by increasing the copy number. Nevertheless,...
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Promoter engineering enables precise metabolic regulation towards efficient β-elemene production in Ogataea polymorpha
June 2024
Precisely controlling gene expression is beneficial for optimizing biosynthetic pathways for improving the production. However, promoters in nonconventional yeasts such as Ogataea polymorpha are always...
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Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production
June 2024
Lactate is an important monomer for the synthesis of poly-lactate (PLA), which is a substitute for the petrochemical plastics. To achieve the goal of high lactate titer, rate, and yield for commercial...
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Construction of a broad-host-range Anderson promoter series and particulate methane monooxygenase promoter variants expand the methanotroph genetic toolbox
June 2024
Methanotrophic bacteria are currently used industrially for the bioconversion of methane-rich natural gas and anaerobic digestion-derived biogas to valuable products. These bacteria may also serve to...
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Construction of an alternative NADPH regeneration pathway improves ethanol production in Saccharomyces cerevisiae with xylose metabolic pathway
June 2024
Full conversion of glucose and xylose from lignocellulosic hydrolysates is required for obtaining a high ethanol yield. However, glucose and xylose share flux in the pentose phosphate pathway (PPP)...
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Descriptor-augmented machine learning for enzyme-chemical interaction predictions
June 2024
Descriptors play a pivotal role in enzyme design for the greener synthesis of biochemicals, as they could characterize enzymes and chemicals from the physicochemical and evolutionary perspective. This...
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The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction
June 2024
Aspergillus niger is an efficient cell factory for organic acids production, particularly l-malic acid, through genetic manipulation. However, the traditional method of collecting A. niger spores for...
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CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans
June 2024
Gluconobacter oxydans is an important Gram-negative industrial microorganism that produces vitamin C and other products due to its efficient membrane-bound dehydrogenase system. Its incomplete oxidation...
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Omics data analysis reveals the system-level constraint on cellular amino acid composition
June 2024
Proteins play a pivotal role in coordinating the functions of organisms, essentially governing their traits, as the dynamic arrangement of diverse amino acids leads to a multitude of folded configurations...
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Multi-modular metabolic engineering of heme synthesis in Corynebacterium glutamicum
June 2024
Heme, an iron-containing porphyrin derivative, holds great promise in fields like medicine, food production and chemicals. Here, we developed an engineered Corynebacterium glutamicum strain for efficient...
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Engineered microorganisms: A new direction in kidney stone prevention and treatment
June 2024
Numerous studies have shown that intestinal and urinary tract flora are closely related to the formation of kidney stones. The removal of probiotics represented by lactic acid bacteria and the colonization...
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Single carbon metabolism – A new paradigm for microbial bioprocesses?
June 2024
Increasing atmospheric carbon dioxide levels, a reduction of arable land area and the dependence of first and second generation biotechnology feedstocks on agricultural products, call for alternative,...
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Design and synthesis of synthetic promoters for consistency of gene expression across growth phases and scale in S. cerevisiae
June 2024
Metabolic engineering and synthetic biology endeavors benefit from promoters that perform consistently (or robustly) with respect to cellular growth phase (exponential and stationary) and fermentation...
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Acarbose glycosylation by AcbE for the production of acarstatins with enhanced α-amylase inhibitory activity
June 2024
Acarbose is a potent glycosidase inhibitor widely used in the clinical treatment of type 2 diabetes mellitus (T2DM). Various acarbose analogs have been identified while exploring compounds with improved...
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Antibacterial activity and mechanism of X33 antimicrobial oligopeptide against Acinetobacter baumannii
June 2024
Acinetobacter baumannii is a pathogenic bacterium widespread in human environments, especially in intensive care units, and is associated with high morbidity and infection rates. Multiple drug resistance...
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Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max
June 2024
Plants produce diverse flavonoids for defense and stress resistance, most of which have health benefits and are widely used as food additives and medicines. Methylation of the free hydroxyl groups of...
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Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production
June 2024
Due to the complicated metabolic and regulatory networks of l-serine biosynthesis and degradation, microbial cell factories for l-serine production using non-model microorganisms have not been reported....
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Engineering and finetuning expression of SerC for balanced metabolic flux in vitamin B6 production
June 2024
Vitamin B6 plays a crucial role in cellular metabolism and stress response, making it an essential component for growth in all known organisms. However, achieving efficient biosynthesis of vitamin B6...
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Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket
June 2024
Fungal bifunctional terpene synthases (BFTSs) catalyze the formation of numerous di-/sester-/tri-terpenes skeletons. However, the mechanism in controlling the cyclization pattern of terpene scaffolds...
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Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters
Available online 5 May 2024
Streptomyces offer a wealth of naturally occurring compounds with diverse structures, many of which possess significant pharmaceutical values. However, new product exploration and increased yield of...
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Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway
March 2024
2′-Fucosyllactose (2′-FL) is one of the important functional oligosaccharides in breast milk. So far, few attempts on biosynthesis of 2′-FL by the salvage pathway have been reported. Herein, the salvage...
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Thank you Reviewers
March 2024
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