High production of polyols using crude glycerol by wild-type safe yeasts

已发布 07 二月, 2024

Utilizing crude glycerol for the synthesis of high-value products offers a promising solution to counter the adverse effects of declining glycerol prices in the biodiesel sector. The prevalence of crude glycerol, a by-product of biodiesel production, across agriculture, biofuel, and industrial sectors is steadily rising. Recent advancements have demonstrated the effectiveness of both wild-type and mutant yeast strains as microbial cell factories capable of converting glycerol into a diverse array of valuable compounds, including microbial oils, sugar-alcohols (polyols), and organic acids. With the projected increase in biodiesel production, there is a need to explore integrated (bio)-processes for the valorization of this residual material, embracing a "bio-refinery approach."

In a new study published in the KeAi journal Carbon Resources Conversion, a team of researchers in Greece reported innovative approaches to harnessing the potential of crude glycerol. They utilized it as a substrate for natural yeasts of the Generally Recognized As Safe (GRAS) species Yarrowia lipolytica. This method resulted in the production of valuable compounds such as sugar-alcohols (mannitol, arabitol, and erythritol), as well as other metabolites including citric acid, yeast biomass, and polysaccharides.

“In the context of the industrial scale, the production of polyols through the use of crude glycerol could significantly contribute to the recycling of this residue that is generated in constantly increasing quantities worldwide, offering a ‘green’ alternative on the protection of the environment, as huge amounts of the mentioned residue can be successfully used to produce high added-value products with a plethora of applications in the food, pharmaceutical and chemical sectors”.

“Therefore, the use of microorganisms is a topic that needs further investigation as it could demonstrate the possibility of various yeasts, to be used as microbial cell factories in various glycerol utilization processes, with the production of biotechnological products and yeast biomass, of utmost importance for food, pharmaceutical and chemical applications,” shares corresponding author Seraphim Papanikolaou. “It is worth emphasizing that the biochemical pathway of polyol synthesis has not yet been fully elucidated, making such a study highly attractive and interesting. Consequently, the production of polyols from industrial by-products is an important area of focus for food biotechnology, offering dual benefits of environmental enhancement and economic advancement.”

Contact author: 1) Eleni-Stavroula Vastaroucha (PhD), Laboratory of Food Microbiology and Biotechnology, Department of Food Science and Human Nutrition, Agricultural University of Athens, ellens.v@hotmail.co.uk; 2) Professor Seraphim Papanikolaou, Laboratory of Food Microbiology and Biotechnology, Department of Food Science and Human Nutrition, Agricultural University of Athens, spapanik@aua.gr

Funder: 1. The current investigation was financially supported by the project entitled “Adding value to biodiesel-derived crude glycerol with the use of Chemical and Microbial Technology” (Acronym: Addvalue2glycerol, project code Τ1ΕΔΚ-03002) financed by the Ministry of National Education and Religious Affairs, Greece (project action: “Investigate – Create – Innovate 2014-2020, Intervention II”).

2. «The implementation of the doctoral thesis was co-financed by Greece and the European Union (European Social Fund-ESF) through the Operational Programme «Human Resources Development, Education and Lifelong Learning» in the context of the Act “Enhancing Human Resources Research Potential by undertaking a Doctoral Research” Sub-action 2: IKY Scholarship Programme for PhD candidates in the Greek Universities».

Method of research

Experimental study

Subject of research

Not applicable

Article Title

Studies of polyol production by the yeast Yarrowia lipolytica growing on crude glycerol under stressful conditions




The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.


Carbon Resources Conversion


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