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Most Downloaded Synthetic and Systems Biotechnology Articles

The most downloaded articles from ScienceDirect in the last 90 days.

1. CRISPR-Cas9/Cas12a biotechnology and application in bacteria

September 2018
Ruilian Yao | Di Liu | Xiao Jia | Yuan Zheng | Wei Liu | Yi Xiao

CRISPR-Cas technologies have greatly reshaped the biology field. In this review, we discuss the CRISPR-Cas with a particular focus on the associated technologies and applications of CRISPR-Cas9 and...

2. Engineering and modification of microbial chassis for systems and synthetic biology

March 2019
Haotian Chi | Xiaoli Wang | Yue Shao | Ying Qin | Zixin Deng | Lianrong Wang | Shi Chen

Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health, medicine, agricultural,...

3. Cell-free synthetic biology: Engineering in an open world

March 2017
Yuan Lu

Cell-free synthetic biology emerges as a powerful and flexible enabling technology that can engineer biological parts and systems for life science applications without using living cells. It provides...

4. Reactive oxygen species and antioxidant properties from mushrooms

March 2017
Carmen Sánchez

Preventive medicine and food industry have shown an increased interest in the development of natural antioxidants, since those most commonly used synthetic antioxidants may have restricted use in food....

5. Transcription control engineering and applications in synthetic biology

September 2017
Michael D. Engstrom | Brian F. Pfleger

In synthetic biology, researchers assemble biological components in new ways to produce systems with practical applications. One of these practical applications is control of the flow of genetic information...

6. A living foundry for Synthetic Biological Materials: A synthetic biology roadmap to new advanced materials

June 2018
Rosalind A. Le Feuvre | Nigel S. Scrutton

Society is on the cusp of harnessing recent advances in synthetic biology to discover new bio-based products and routes to their affordable and sustainable manufacture. This is no more evident than...

7. Engineering bacteria for enhanced polyhydroxyalkanoates (PHA) biosynthesis

September 2017
Guo-Qiang Chen | Xiao-Ran Jiang

Polyhydroxyalkanoates (PHA) have been produced by some bacteria as bioplastics for many years. Yet their commercialization is still on the way. A few issues are related to the difficulty of PHA commercialization:...

8. Fundamental CRISPR-Cas9 tools and current applications in microbial systems

September 2017
Pingfang Tian | Jia Wang | Xiaolin Shen | Justin Forrest Rey | Qipeng Yuan | Yajun Yan

Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we...

9. Activation of microbial secondary metabolic pathways: Avenues and challenges

September 2018
Bikash Baral | Amir Akhgari | Mikko Metsä-Ketelä

Microbial natural products are a tremendous source of new bioactive chemical entities for drug discovery. Next generation sequencing has revealed an unprecedented genomic potential for production of...

10. Blossom of CRISPR technologies and applications in disease treatment

December 2018
Huayi Liu | Lian Wang | Yunzi Luo

Since 2013, the CRISPR-based bacterial antiviral defense systems have revolutionized the genome editing field. In addition to genome editing, CRISPR has been developed as a variety of tools for gene...

11. A review of computational tools for design and reconstruction of metabolic pathways

December 2017
Lin Wang | Satyakam Dash | Chiam Yu Ng | Costas D. Maranas

Metabolic pathways reflect an organism's chemical repertoire and hence their elucidation and design have been a primary goal in metabolic engineering. Various computational methods have been developed...

12. Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001

September 2019
Sagarika Devi | Heiko T. Kiesewalter | Renátó Kovács | Jens Christian Frisvad | Tilmann Weber | Thomas Ostenfeld Larsen | Ákos T. Kovács | Ling Ding

For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced...

13. The secondary metabolite bioinformatics portal: Computational tools to facilitate synthetic biology of secondary metabolite production

June 2016
Tilmann Weber | Hyun Uk Kim

Natural products are among the most important sources of lead molecules for drug discovery. With the development of affordable whole-genome sequencing technologies and other ‘omics tools, the field...

14. Benchmarking two Saccharomyces cerevisiae laboratory strains for growth and transcriptional response to methanol

December 2019
Monica I. Espinosa | Thomas C. Williams | Isak S. Pretorius | Ian T. Paulsen

One-carbon compounds, such as methanol, are becoming potential alternatives to sugars as feedstocks for the biological production of chemicals, fuels, foods, and pharmaceuticals. Efficient biological...

15. A brief overview of synthetic biology research programs and roadmap studies in the United States

December 2016
Tong Si | Huimin Zhao

The United States is a leading nation in the development of synthetic biology, an emerging engineering discipline to create, control and reprogram biological systems. With strategic investment from...

16. Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation

March 2019
Lorna Suckling | Ciaran McFarlane | Chelsea Sawyer | Stephen P. Chambers | Richard I. Kitney | David W. McClymont | Paul S. Freemont

High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories. In particular, it is an essential quality control...

17. CRISPy-web: An online resource to design sgRNAs for CRISPR applications

June 2016
Kai Blin | Lasse Ebdrup Pedersen | Tilmann Weber | Sang Yup Lee

CRISPR/Cas9-based genome editing has been one of the major achievements of molecular biology, allowing the targeted engineering of a wide range of genomes. The system originally evolved in prokaryotes...

18. Development of fungal cell factories for the production of secondary metabolites: Linking genomics and metabolism

March 2017
Jens Christian Nielsen | Jens Nielsen

The genomic era has revolutionized research on secondary metabolites and bioinformatics methods have in recent years revived the antibiotic discovery process after decades with only few new active molecules...

19. Synthetic biology of microbes synthesizing polyhydroxyalkanoates (PHA)

December 2016
Guo-Qiang Chen | Xiao-Ran Jiang | Yingying Guo

Microbial polyhydroxyalkanoates (PHA) have been produced as bioplastics for various purposes. Under the support of China National Basic Research 973 Project, we developed synthetic biology methods to...

20. Therapeutic applications of genetic code expansion

September 2018
Yujia Huang | Tao Liu

In nature, a limited, conservative set of amino acids are utilized to synthesize proteins. Genetic code expansion technique reassigns codons and incorporates noncanonical amino acids (ncAAs) through...

21. Methods to reduce variability in E. Coli-based cell-free protein expression experiments

December 2019
Jared L. Dopp | Yeong Ran Jo | Nigel F. Reuel

Cell-free protein synthesis (CFPS) is an established biotechnology tool that has shown great utility in many applications such as prototyping proteins, building genetic circuits, designing biosensors,...

22. Prospective study of probiotic supplementation results in immune stimulation and improvement of upper respiratory infection rate

June 2018
Hong Zhang | Chiajung Yeh | Zonglian Jin | Liwei Ding | Bryan Y. Liu | Li Zhang | H. Kathleen Dannelly

The human gut microbiota is an important environmental factor for human health with evolutionarily conserved roles in immunity, metabolism, development, and behavior of the host. Probiotic organisms...

23. Regulation of antibiotic biosynthesis in actinomycetes: Perspectives and challenges

December 2018
Junhong Wei | Lang He | Guoqing Niu

Actinomycetes are the main sources of antibiotics. The onset and level of production of each antibiotic is subject to complex control by multi-level regulators. These regulators exert their functions...

24. Metabolic engineering for the microbial production of isoprenoids: Carotenoids and isoprenoid-based biofuels

September 2017
Fu-Xing Niu | Qian Lu | Yi-Fan Bu | Jian-Zhong Liu

Isoprenoids are the most abundant and highly diverse group of natural products. Many isoprenoids have been used for pharmaceuticals, nutraceuticals, flavors, cosmetics, food additives and biofuels....

25. Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products

June 2018
Jian Li | Lingkai Zhang | Wanqiu Liu

Natural products with significant biological activities continuously act as rich sources for drug discovery and development. To harness the potential of these valuable compounds, robust methods need...

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