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Most Downloaded Synthetic and Systems Biotechnology Articles

The most downloaded articles from ScienceDirect in the last 90 days.

1. CRISPR-Cas9/Cas12a biotechnology and application in bacteria

September 2018
Ruilian Yao | Di Liu | Xiao Jia | Yuan Zheng | Wei Liu | Yi Xiao

CRISPR-Cas technologies have greatly reshaped the biology field. In this review, we discuss the CRISPR-Cas with a particular focus on the associated technologies and applications of CRISPR-Cas9 and...

2. CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants

December 2020
Abdullah | Zhengzheng Jiang | Xulin Hong | Shun Zhang | Ruilian Yao | Yi Xiao

CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing. The RNA guided Cas nucleases generate DNA...

3. Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

June 2021
Jucan Gao | Lihong Jiang | Jiazhang Lian

The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P. pastoris...

4. Cell-free synthetic biology: Engineering in an open world

March 2017
Yuan Lu

Cell-free synthetic biology emerges as a powerful and flexible enabling technology that can engineer biological parts and systems for life science applications without using living cells. It provides...

5. Engineering and modification of microbial chassis for systems and synthetic biology

March 2019
Haotian Chi | Xiaoli Wang | Yue Shao | Ying Qin | Zixin Deng | Lianrong Wang | Shi Chen

Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health, medicine, agricultural,...

6. Fundamental CRISPR-Cas9 tools and current applications in microbial systems

September 2017
Pingfang Tian | Jia Wang | Xiaolin Shen | Justin Forrest Rey | Qipeng Yuan | Yajun Yan

Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we...

7. Systematic optimization of the yeast cell factory for sustainable and high efficiency production of bioactive ginsenoside compound K

June 2021
Pingping Wang | Jiali Wang | Guoping Zhao | Xing Yan | Zhihua Zhou

Ginsenoside Compound K (CK) has been recognized as a major functional component that is absorbed into the systemic circulation after oral administration of ginseng. CK demonstrates diverse bioactivities....

8. Engineering bacteria for enhanced polyhydroxyalkanoates (PHA) biosynthesis

September 2017
Guo-Qiang Chen | Xiao-Ran Jiang

Polyhydroxyalkanoates (PHA) have been produced by some bacteria as bioplastics for many years. Yet their commercialization is still on the way. A few issues are related to the difficulty of PHA commercialization:...

9. The secondary metabolite bioinformatics portal: Computational tools to facilitate synthetic biology of secondary metabolite production

June 2016
Tilmann Weber | Hyun Uk Kim

Natural products are among the most important sources of lead molecules for drug discovery. With the development of affordable whole-genome sequencing technologies and other ‘omics tools, the field...

10. Chemical modifications of proteins and their applications in metalloenzyme studies

March 2021
Nathchar Naowarojna | Ronghai Cheng | Juan Lopez | Christina Wong | Lu Qiao | Pinghua Liu

Protein chemical modifications are important tools for elucidating chemical and biological functions of proteins. Several strategies have been developed to implement these modifications, including enzymatic...

11. Methodologies for preparation of prokaryotic extracts for cell-free expression systems

December 2020
Stephanie D. Cole | Aleksandr E. Miklos | Abel C. Chiao | Zachary Z. Sun | Matthew W. Lux

Cell-free systems that mimic essential cell functions, such as gene expression, have dramatically expanded in recent years, both in terms of applications and widespread adoption. Here we provide a review...

12. Comparative metagenomic analysis of the vaginal microbiome in healthy women

June 2021
Fei Liu | Yingshun Zhou | Lisi Zhu | Zhiyi Wang | LiangKun Ma | Yuan He | Pengcheng Fu

The composition of these vaginal microbiome has a significant impact on women's health. However, few studies have characterized the vaginal microbiome of healthy Chinese women using metagenomic sequencing....

13. The Fold-Illuminator: A low-cost, portable, and disposable incubator-illuminator device

June 2021
Logan R. Burrington | Emran Baryal | Katherine Hui | Emmett Lambert | Sarah T. Harding | Javin P. Oza

Fluorescent reporters have revolutionized modern applications in the fields of molecular and synthetic biology, enabling applications ranging from education to point-of-care diagnostics. Past advancements...

14. Screening neutral sites for metabolic engineering of methylotrophic yeast Ogataea polymorpha

June 2021
Wei Yu | Jiaoqi Gao | Xiaoxin Zhai | Yongjin J. Zhou

Methylotrophic yeast Ogataea polymorpha is capable to utilize multiple carbon feedstocks especially methanol as sole carbon source and energy, making it an ideal host for bio-manufacturing. However,...

15. Microbial soluble aromatic prenyltransferases for engineered biosynthesis

June 2021
He-Ping Chen | Ikuro Abe

Prenyltransferase (PTase) enzymes play crucial roles in natural product biosynthesis by transferring isoprene unit(s) to target substrates, thereby generating prenylated compounds. The prenylation step...

16. Transcription control engineering and applications in synthetic biology

September 2017
Michael D. Engstrom | Brian F. Pfleger

In synthetic biology, researchers assemble biological components in new ways to produce systems with practical applications. One of these practical applications is control of the flow of genetic information...

17. Expanding application of CRISPR-Cas9 system in microorganisms

December 2020
Jing Zhao | Huan Fang | Dawei Zhang

The development of CRISPR-Cas9 based genetic manipulation tools represents a huge breakthrough in life sciences and has been stimulating research on metabolic engineering, synthetic biology, and systems...

18. PCPD: Plant cytochrome P450 database and web-based tools for structural construction and ligand docking

June 2021
Hui Wang | Qian Wang | Yuqian Liu | Xiaoping Liao | Huanyu Chu | Hong Chang | Yang Cao | Zhigang Li | Tongcun Zhang | Jian Cheng | Huifeng Jiang

Plant cytochrome P450s play key roles in the diversification and functional modification of plant natural products. Although over 200,000 plant P450 gene sequences have been recorded, only seven crystalized...

19. Activation of microbial secondary metabolic pathways: Avenues and challenges

September 2018
Bikash Baral | Amir Akhgari | Mikko Metsä-Ketelä

Microbial natural products are a tremendous source of new bioactive chemical entities for drug discovery. Next generation sequencing has revealed an unprecedented genomic potential for production of...

20. A review of computational tools for design and reconstruction of metabolic pathways

December 2017
Lin Wang | Satyakam Dash | Chiam Yu Ng | Costas D. Maranas

Metabolic pathways reflect an organism's chemical repertoire and hence their elucidation and design have been a primary goal in metabolic engineering. Various computational methods have been developed...

21. Challenges and advances in genome mining of ribosomally synthesized and post-translationally modified peptides (RiPPs)

September 2020
Zheng Zhong | Beibei He | Jie Li | Yong-Xin Li

Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a class of cyclic or linear peptidic natural products with remarkable structural and functional diversity. Recent advances...

22. Emerging biosensors in detection of natural products

December 2020
Firoozeh Piroozmand | Fatemeh Mohammadipanah | Farnoush Faridbod

Natural products (NPs) are a valuable source in the food, pharmaceutical, agricultural, environmental, and many other industrial sectors. Their beneficial properties along with their potential toxicities...

23. Biofilm architecture: An emerging synthetic biology target

March 2020
Stephen J. Kassinger | Monique L. van Hoek

Synthetic biologists are exploiting biofilms as an effective mechanism for producing various outputs. Metabolic optimization has become commonplace as a method of maximizing system output. In addition...

24. Production of anthocyanins in metabolically engineered microorganisms: Current status and perspectives

December 2017
Jian Zha | Mattheos A.G. Koffas

Microbial production of plant-derived natural products by engineered microorganisms has achieved great success thanks to large extend to metabolic engineering and synthetic biology. Anthocyanins, the...

25. CRISPy-web: An online resource to design sgRNAs for CRISPR applications

June 2016
Kai Blin | Lasse Ebdrup Pedersen | Tilmann Weber | Sang Yup Lee

CRISPR/Cas9-based genome editing has been one of the major achievements of molecular biology, allowing the targeted engineering of a wide range of genomes. The system originally evolved in prokaryotes...

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