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Most Downloaded Synthetic and Systems Biotechnology Articles

The most downloaded articles from ScienceDirect in the last 90 days.

1. CRISPR-Cas9/Cas12a biotechnology and application in bacteria

September 2018
Ruilian Yao | Di Liu | Xiao Jia | Yuan Zheng | Wei Liu | Yi Xiao

CRISPR-Cas technologies have greatly reshaped the biology field. In this review, we discuss the CRISPR-Cas with a particular focus on the associated technologies and applications of CRISPR-Cas9 and...

2. Prudently conduct the engineering and synthesis of the SARS-CoV-2 virus

June 2020
Peng Gao | Shiwen Ma | Daru Lu | Carl Mitcham | Yijia Jing | Guoyu Wang

In response to the outbreak of COVID-19 that has been sweeping the world, scientists reconstructed the SARS-CoV-2 rapidly using a synthetic genomics platform, in order to accelerate therapeutics and...

3. Fundamental CRISPR-Cas9 tools and current applications in microbial systems

September 2017
Pingfang Tian | Jia Wang | Xiaolin Shen | Justin Forrest Rey | Qipeng Yuan | Yajun Yan

Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we...

4. Engineering and modification of microbial chassis for systems and synthetic biology

March 2019
Haotian Chi | Xiaoli Wang | Yue Shao | Ying Qin | Zixin Deng | Lianrong Wang | Shi Chen

Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health, medicine, agricultural,...

5. Cell-free synthetic biology: Engineering in an open world

March 2017
Yuan Lu

Cell-free synthetic biology emerges as a powerful and flexible enabling technology that can engineer biological parts and systems for life science applications without using living cells. It provides...

6. Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases

June 2020
Erin E. Drufva | Elijah G. Hix | Constance B. Bailey

Modular polyketide synthases (PKSs) are a multidomain megasynthase class of biosynthetic enzymes that have great promise for the development of new compounds, from new pharmaceuticals to high value...

7. A review of computational tools for design and reconstruction of metabolic pathways

December 2017
Lin Wang | Satyakam Dash | Chiam Yu Ng | Costas D. Maranas

Metabolic pathways reflect an organism's chemical repertoire and hence their elucidation and design have been a primary goal in metabolic engineering. Various computational methods have been developed...

8. Challenges and advances in genome mining of ribosomally synthesized and post-translationally modified peptides (RiPPs)

September 2020
Zheng Zhong | Beibei He | Jie Li | Yong-Xin Li

Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a class of cyclic or linear peptidic natural products with remarkable structural and functional diversity. Recent advances...

9. Reactive oxygen species and antioxidant properties from mushrooms

March 2017
Carmen Sánchez

Preventive medicine and food industry have shown an increased interest in the development of natural antioxidants, since those most commonly used synthetic antioxidants may have restricted use in food....

10. The secondary metabolite bioinformatics portal: Computational tools to facilitate synthetic biology of secondary metabolite production

June 2016
Tilmann Weber | Hyun Uk Kim

Natural products are among the most important sources of lead molecules for drug discovery. With the development of affordable whole-genome sequencing technologies and other ‘omics tools, the field...

11. A living foundry for Synthetic Biological Materials: A synthetic biology roadmap to new advanced materials

June 2018
Rosalind A. Le Feuvre | Nigel S. Scrutton

Society is on the cusp of harnessing recent advances in synthetic biology to discover new bio-based products and routes to their affordable and sustainable manufacture. This is no more evident than...

12. A brief overview of synthetic biology research programs and roadmap studies in the United States

December 2016
Tong Si | Huimin Zhao

The United States is a leading nation in the development of synthetic biology, an emerging engineering discipline to create, control and reprogram biological systems. With strategic investment from...

13. Biofilm architecture: An emerging synthetic biology target

March 2020
Stephen J. Kassinger | Monique L. van Hoek

Synthetic biologists are exploiting biofilms as an effective mechanism for producing various outputs. Metabolic optimization has become commonplace as a method of maximizing system output. In addition...

14. Improving the reaction mix of a Pichia pastoris cell-free system using a design of experiments approach to minimise experimental effort

September 2020
Alex J. Spice | Rochelle Aw | Daniel G. Bracewell | Karen M. Polizzi

A renaissance in cell-free protein synthesis (CFPS) is underway, enabled by the acceleration and adoption of synthetic biology methods. CFPS has emerged as a powerful platform technology for synthetic...

15. Discovery and modification of cytochrome P450 for plant natural products biosynthesis

September 2020
Xiaonan Liu | Xiaoxi Zhu | Hui Wang | Tian Liu | Jian Cheng | Huifeng Jiang

Cytochrome P450s are widespread in nature and play key roles in the diversification and functional modification of plant natural products. Over the last few years, there has been remarkable progress...

16. Activation of microbial secondary metabolic pathways: Avenues and challenges

September 2018
Bikash Baral | Amir Akhgari | Mikko Metsä-Ketelä

Microbial natural products are a tremendous source of new bioactive chemical entities for drug discovery. Next generation sequencing has revealed an unprecedented genomic potential for production of...

17. Coordinated regulation for nature products discovery and overproduction in Streptomyces

June 2020
Qun Zhou | Shuqing Ning | Yunzi Luo

Streptomyces is an important treasure trove for natural products discovery. In recent years, many scientists focused on the genetic modification and metabolic regulation of Streptomyces to obtain diverse...

18. CRISPy-web: An online resource to design sgRNAs for CRISPR applications

June 2016
Kai Blin | Lasse Ebdrup Pedersen | Tilmann Weber | Sang Yup Lee

CRISPR/Cas9-based genome editing has been one of the major achievements of molecular biology, allowing the targeted engineering of a wide range of genomes. The system originally evolved in prokaryotes...

19. Engineering bacteria for enhanced polyhydroxyalkanoates (PHA) biosynthesis

September 2017
Guo-Qiang Chen | Xiao-Ran Jiang

Polyhydroxyalkanoates (PHA) have been produced by some bacteria as bioplastics for many years. Yet their commercialization is still on the way. A few issues are related to the difficulty of PHA commercialization:...

20. An aurora of natural products-based drug discovery is coming

June 2020
Yaojun Tong | Zixin Deng

Natural products (NPs), a nature's reservoir possessing enormous structural and functional diversity far beyond the current ability of chemical synthesis, are now proving themselves as most wonderful...

21. Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001

September 2019
Sagarika Devi | Heiko T. Kiesewalter | Renátó Kovács | Jens Christian Frisvad | Tilmann Weber | Thomas Ostenfeld Larsen | Ákos T. Kovács | Ling Ding

For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced...

22. Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast

March 2020
Tadas Jakočiūnas | Andreas K. Klitgaard | Eftychia Eva Kontou | Julie Bang Nielsen | Emil Thomsen | David Romero-Suarez | Kai Blin | Christopher J. Petzold | Jennifer W. Gin | Yaojun Tong | Charlotte Held Gotfredsen | Pep Charusanti | Rasmus J.N. Frandsen | Tilmann Weber | Sang Yup Lee | Michael K. Jensen | Jay D. Keasling

To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new,...

23. Therapeutic applications of genetic code expansion

September 2018
Yujia Huang | Tao Liu

In nature, a limited, conservative set of amino acids are utilized to synthesize proteins. Genetic code expansion technique reassigns codons and incorporates noncanonical amino acids (ncAAs) through...

24. Prospective study of probiotic supplementation results in immune stimulation and improvement of upper respiratory infection rate

June 2018
Hong Zhang | Chiajung Yeh | Zonglian Jin | Liwei Ding | Bryan Y. Liu | Li Zhang | H. Kathleen Dannelly

The human gut microbiota is an important environmental factor for human health with evolutionarily conserved roles in immunity, metabolism, development, and behavior of the host. Probiotic organisms...

25. Simple glycolipids of microbes: Chemistry, biological activity and metabolic engineering

March 2018
Ahmad Mohammad Abdel-Mawgoud | Gregory Stephanopoulos

Glycosylated lipids (GLs) are added-value lipid derivatives of great potential. Besides their interesting surface activities that qualify many of them to act as excellent ecological detergents, they...

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