Combinatorial metabolic engineering of Mycobacterium neoaurum LY-2 for efficient androstenedione production from phytosterols
April 2027
Androstenedione (AD) is an important precursor for the synthesis of corticosteroids, sex hormones, and other high-value steroid pharmaceuticals. Microbial conversion of phytosterols provides a sustainable...
Genome-integrated engineering of Escherichia coli Nissle 1917 enables sustained gut-local biosynthesis of 5-hydroxytryptophan
April 2027
5-Hydroxytryptophan (5-HTP) is a functional precursor of serotonin; however, achieving sustained 5-HTP production in vivo by engineered microbes remains challenging. To address this, we developed a...
Strategies and applications for alleviating carbon catabolite repression
April 2027
Carbon catabolite repression (CCR) is a widespread regulatory strategy across diverse microorganisms that prioritises the utilisation of preferred carbon sources. In industrial bioprocesses, however,...
Reassigning an amber codon to selenocysteine using a functional cysteine suppression pair in Escherichia coli
March 2027
Selenoproteins, characterized by the presence of selenocysteine (Sec) residues, are widely distributed across all domains of life. The unique attributes of selenoproteins are conferred by Sec, which...
Transfer of chloroplast rbcL and atpB genes to nucleus enables partial rescue of photoautotrophic growth of Chlamydomonas
March 2027
An ancient cyanobacterium has evolved through endosymbiosis to form extant chloroplasts of eukaryotic algae and higher plants. During this process, most genes have been transferred to the nuclear genomes....
System reconstruction of Bacillus licheniformis for efficient expression of alkaline protease
March 2027
Industrial enzymes are widely used in diverse applications, but low productivity limits their further widespread utilization. This research aimed to develop high-performance alkaline protease (AprE)...
Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
March 2027
Dalbavancin is a potent next-generation lipoglycopeptide antibiotic, but the biosynthesis of its essential precursor, A40926B0, is severely hindered in its native producer Nonomuraea gerenzanensis....
An orthogonal transcription system enables high-efficiency rescue of measles virus-based vaccine vectors against multiple pathogens
March 2027
Recombinant measles virus (rMeV) vectors are promising platforms for vaccine development against emerging infectious diseases due to their safety, stability, and potent immunogenicity. However, conventional...
Systematic evaluation of the DNA repair machinery facilitated the development of efficient genome editing tools for the red yeast Rhodotorula toruloides
March 2027
The red yeast Rhodotorula toruloides is a robust lipid producer, and its biotechnology applications have been expanded through genetic engineering based on the non-homologous end joining (NHEJ) pathway....
EINN: An enzyme-informed neural network guided by an enzyme-constrained genome-scale metabolic model
March 2027
Predicting cellular metabolism from molecular data is a central challenge in systems biology, with direct implications for engineering microbial cell factories. Genome-scale metabolic models (GEMs)...
Toward engineered microbial chassis for growth–production balancing
March 2027
The inherent conflict between cellular growth and product synthesis, arising from limited resource allocation and metabolic burden, fundamentally constrains the performance of microbial chassis. Traditional...
Metabolic engineering of Escherichia coli for efficient production of d-phenylglycine
March 2027
d-phenylglycine (D-PHG) is a valuable building block extensively used in the synthesis of β-lactam antibiotics and other high-value pharmaceuticals. However, its conventional chemical production relies...
Alternative splicing-induced thioesterase domain deficiency drives the production of natural orsellinic acid derivatives with a pantetheine moiety in nonreducing polyketide synthases
March 2027
Nonreducing polyketide synthase Preu6 catalyzes didepside formation by interaction of starter acyl transferase and thioesterase (TE) domains. Here, we show that an aberrant transcript, preu6β, is generated...
Unlocking raw-corncob consolidated bioprocessing for malic acid production by DyP-enabled lignin deconstruction in Myceliophthora thermophila
March 2027
l-Malic acid is an important C4 platform chemical, but its direct production from raw lignocellulosic biomass by consolidated bioprocessing (CBP) remains constrained by biomass recalcitrance. Here,...
Deep learning-driven de novo design of ribosome binding sites in Paracoccus denitrificans
March 2027
Paracoccus denitrificans is a heterotrophic nitrifying–aerobic denitrifying bacterium widely used in wastewater treatment and as a model system for studying electron transport chains, making it a promising...
Gentamicin and d-serine selective pressure to improve the homogeneity of cells expressing target proteins
March 2027
In this work, selective pressure was applied in E. coli to enrich the population expressing recombinant proteins and suppress the emergence of low-expression phenotypes. To this end, plasmids were constructed...
Systems-level analysis of 1,6-hexanediol stress response and tolerance engineering in Escherichia coli
March 2027
1,6-Hexanediol (1,6-HDO) is an important chemical platform widely used in polymer and pharmaceutical industries, while its toxicity remains a major bottleneck limiting efficient microbial production....
Metabolic state-driven monitoring and control of abnormal pigment formation in sodium gluconate fermentation by Aspergillus niger
February 2027
Abnormal pigment formation during late-stage fungal fermentation poses a significant challenge to industrial product quality. This study established an integrated framework to understand and control...
A user-friendly strategy to engineer tailored intermediate strains for overcoming combined type I and type II restriction-modification barriers in Staphylococcus aureus
February 2027
A significant proportion of Staphylococcus aureus clinical isolates are refractory to plasmid transformation due to the combined action of type I and type II restriction-modification (RM) systems. Current...
Quorum sensing-driven riboflavin-hyperproducing electroactive bacteria for enhanced bioelectricity generation from sludge: From modular optimization to process performance
February 2027
Waste activated sludge (WAS) represents a significant byproduct of wastewater treatment and a renewable resource for bioenergy. Bioelectrochemical systems (BESs), which couple microbial metabolism with...
Lactiplantibacillus plantarum as a multifunctional probiotic for inflammatory bowel disease: Mechanisms, synbiotic strategies, and delivery systems
February 2027
Inflammatory bowel disease (IBD) encompasses a group of chronic inflammatory disorders of the bowel, characterized by complex etiologies that are closely associated with an imbalance in intestinal microbiota,...
High-level amyrin production in Yarrowia lipolytica via metabolic and enzyme engineering
February 2027
Amyrins arе pеntacyclic tritеrpеnoids with valuablе pharmacеutical and cosmеtic applications; howеvеr, thеir low abundancе in plants and complеx еxtraction procеssеs hindеr sustainablе supply. Hеrе,...
scYeast: a biological-knowledge-guided foundation model on yeast single-cell transcriptomics
February 2027
Though large-scale pre-trained models are vital for foundational cell modeling, most of them focus on human or mouse systems, with less emphasis on model organisms like yeast (Saccharomyces cerevisiae),...
Engineering nonconventional yeasts to produce carboxylic acids: Advances and Prospects
February 2027
Due to their versatile carboxy group, carboxylic acids can be used as bio-based monomers for high-value-added industrial products with great market demand. In recent years, metabolic engineering of...
Systematic enzyme and cofactor engineering for efficient ursolic acid biosynthesis in Yarrowia lipolytica
February 2027
Ursolic acid (UA) is a pharmaceutically valuable pentacyclic triterpenoid, but its microbial production is constrained by inefficient cytochrome P450 catalysis and limited cofactor availability. Here,...