Precise l-threonine-to-l-isoleucine pathway regulation for engineering high-efficiency whole-cell biocatalysts
June 2026
l-Isoleucine (L-Ile), a critical branched-chain amino acid with diverse applications in food, pharmaceutical, and cosmetic industries, is difficult to produce efficiently at scale in microbial systems...
Truncation of LPD1 promoter and adaptive evolution increase cytosolic acetyl-CoA supply in yeast
June 2026
Acetyl-CoA is a central metabolic intermediate that serves as a key precursor for the biosynthesis of high-value compounds such as terpenoids. However, its compartmentalization within Saccharomyces...
Combinatorial engineering of enzyme and pathway for efficient β-farnesene bioproduction in Yarrowia lipolytica
June 2026
β-farnesene, a natural sesquiterpene compound, has gained significant attention due to its versatile applications in agriculture, industry, biofuels, and related fields. Microbial biosynthesis offers...
Multi-omics elucidation of resource allocation for enhanced ethanol production via precise glucose control in anaerobic Saccharomyces cerevisiae fermentation
June 2026
Ethanol, a high-demand clean energy source, is primarily produced via fed-batch fermentation in industrial settings. Although our previous study identified an optimal glucose concentration of 30 g/L...
High-level production of vitamin K2 in Escherichia coli via modular molecular engineering
June 2026
Menaquinone-7 (MK-7), a key form of vitamin K2 with wide-ranging nutritional and pharmaceutical applications, has attracted increasing interest for microbial production. Here, we developed an integrated...
Engineered dual-fluorescence functional nucleic acid-based CRISPR/Cas12a biosensor for label-free ratiometric detection of site-specific DNA methylation
June 2026
The CRISPR/Cas12a system holds significant promise for biomedical applications. Nevertheless, the commonly used reporter, fluorophore–quencher-labeled substrates, is hindered by labor-intensive synthesis...
Malic enzyme-based system for transhydrogenation between nicotinamide cofactors
March 2026
The nicotinamide cofactors including nicotinamide adenine dinucleotide (NAD) and its phosphate (NADP) play important roles in facilitating redox reactions for energy metabolism and biosynthesis. To...
Combination of enzyme engineering and quorum sensing system for efficient de novo biosynthesis of β-arbutin in Escherichia coli
March 2026
β-Arbutin is a plant-derived glycoside with various biological activities, which has been applied in pharmaceuticals and whitening cosmetics. Uneven carbon flux distribution and the low catalytic efficiency...
Topoisomerase II-Like variants enhance acid tolerance and low-pH l-malic acid biosynthesis in metabolic engineered Aspergillus nidulans
March 2026
l-malic acid is a versatile compound having extensive applications across food, pharmaceutical, medical, and chemical industries while its production from microbial fermentation offers sustainable advantages...
Biofilm-based continuous secretion of human lysozyme through cell-surface display of FimH on Saccharomyces cerevisiae
March 2026
Biofilms enhance microbial tolerance to harsh environments while preserving cellular activity over prolonged periods. Although biofilm-based continuous fermentation is widely applied for production...
Hfq modulates system-wide regulatory networks controlling biocontrol property in Lysobacter enzymogenes
March 2026
Lysobacter enzymogenes is a beneficial soil bacterium renowned for its potent biocontrol properties, primarily attributed to antimicrobial secondary metabolites such as HSAF and WAP-8294A2, as well...
Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production in Yarrowia lipolytica
March 2026
Succinic acid, an essential platform chemical with extensive utility in biodegradable materials, pharmaceuticals, and the food industry, faces challenges of high energy consumption and environmental...
A near-infrared ratio fluorescent probe achieves mitochondrial hydrogen polysulfide imaging for monitoring ferroptosis in arthritis
March 2026
Reactive sulfur (RSS) is a type of sulfur-containing molecule widely present in biological systems. Hydrogen polysulfide (H2Sn, n > 1), as a member of the active sulfur family, plays an indispensable...
Construction and analysis of a cell factory for terpenoid biosynthesis in Pichia pastoris via metabolic engineering and metabolomics
March 2026
Terpenoids are widely distributed in nature and have various applications in health products, pharmaceuticals, and fragrances. Despite the tremendous potential of terpenoids, traditional production...
Enhanced vitamin B6 production in engineered Escherichia coli via restricted mix-carbon feeding and pressure-controlled fermentation
March 2026
Pyridoxine (PN), a major commercial form of vitamin B6, is mainly produced via chemical synthesis, raising environmental concerns. Microbial fermentation offers a greener alternative, but low biomass...
A rapid combinatorial assembly method for gene cluster characterisation illuminates glidobactin biosynthesis
March 2026
Heterologous expression of natural product gene clusters in tractable hosts offers great promise for achieving sustainable production of nature-inspired drugs. However, it is common for the roles of...
Engineering riboflavin-overproducing Bacillus subtilis via pathway gene overexpression
March 2026
Overexpression of genes at key metabolic nodes frequently enhances the yield of target products. However, this often leads to widespread issues with growth defect and plasmid instability. This study...
Identification of a novel β-1,4-galactosyltransferase from Haemophilus parainfluenzae for efficient biosynthesis of lacto-N-neotetraose in Escherichia coli
March 2026
As an important member of human milk oligosaccharides (HMOs), lacto-N-neotetraose (LNnT) has been recognized as a promising HMO with many physiological activities for infants. β-1,4-Galactosyltransferases...
Efficient trans-aconitic acid production using systematically metabolic engineered Escherichia coli
March 2026
trans-Aconitic acid (TAA) is a versatile platform biochemical exhibiting extensive applications. In this work, Escherichia coli W3110 (DE3) was metabolic modified for the de novo biosynthesis of TAA....
Rapid detection of genotoxicity employing an engineered high-sensitivity and tight SOS-response biosensor in Escherichia coli MG1655
March 2026
Industrial wastewater and pesticide residues contain numerous genotoxic compounds that pose serious health risks by causing DNA damage and potentially triggering carcinogenesis. Conventional analytical...
The coupling effect of Penicillium baileys W2 in the Aspergillus flavus inhibition and peanut growth promotion
March 2026
Aspergillus flavus is a significant plant pathogen, and peanut crops are particularly vulnerable to aflatoxin contamination. This vulnerability underscores the need for more effective control methods....
Systems metabolic engineering of dual pathways with stage-specific activation for high-level 5-aminolevulinic acid production in Escherichia coli
March 2026
5-Aminolevulinic acid (5-ALA) is an important non-proteinogenic amino acid with wide applications in agriculture and medicine. To achieve high-level microbial production, this study established a dual-pathway...
MicroDFBEST: A dCas12b-derived dual-function base editor with programmable editing characteristics for microbial genetic engineering
March 2026
Base editors (BEs) enable precise genome editing, but their use in microbes remains limited by restricted mutagenesis capabilities and narrow editing windows. Here, we reported MicroDFBEST, a novel...
Engineering Yarrowia lipolytica as a yeast cell factory for the de novo production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate)
March 2026
The oleaginous yeast Yarrowia lipolytica presents a promising chassis for the production of biodegradable plastic polyhydroxyalkanoates (PHAs) due to its advantages in precursor supply capabilities...
Enhanced PrrAB system activation and restricted farnesyl pyrophosphate diversion underlie high coenzyme Q10 accumulation in Rhodobacter sphaeroides HY01
March 2026
The industrial Rhodobacter sphaeroides HY01 accumulates an exceptionally high level of coenzyme Q10 (Q10), but the underlying mechanisms remain incompletely understood. Given the central role of Q10...