Metabolic state-driven monitoring and control of abnormal pigment formation in sodium gluconate fermentation by Aspergillus niger
February 2027
Abnormal pigment formation during late-stage fungal fermentation poses a significant challenge to industrial product quality. This study established an integrated framework to understand and control...
A user-friendly strategy to engineer tailored intermediate strains for overcoming combined type I and type II restriction-modification barriers in Staphylococcus aureus
February 2027
A significant proportion of Staphylococcus aureus clinical isolates are refractory to plasmid transformation due to the combined action of type I and type II restriction-modification (RM) systems. Current...
Terpene synthases screening in agarwood tree Aquilaria yunnanensis leads to the discovery of agarospirol
January 2027
Agarwood, renowned as one of the most valuable natural materials in the world of fragrance, is notoriously difficult and time-consuming to obtain, creating a critical bottleneck for its utilization...
Metabolic engineering of Escherichia coli based on adaptive evolution and omics technology for highly efficient l-valine production under oxygen-limited conditions
January 2027
In industrial production, the yield of desired targets derived from carbon sources is frequently diminished by the competitive influence of cellular metabolism within microbial cell factories. The bioproduction...
Data-driven optimization of salt shock for high-efficiency ectoine production in Halomonas elongata
January 2027
Halomonas elongata stands as a highly promising industrial chassis, capable of low-cost fermentation under open, non-sterile conditions. However, precisely regulating the fermentation process to breach...
Evolution of alcohol oxidase for improved methanol bioconversion and formaldehyde tolerance
January 2027
Methanol oxidation by alcohol oxidases (AOXs) is a key bottleneck in one-carbon (C1) bioconversion due to limited catalytic efficiency and poor formaldehyde tolerance. Here, we report the directed evolution...
Key molecular networks underlying retinol's biphasic effects on cell proliferation through multi-omics integrative analysis using genome-scale metabolic modeling
January 2027
Retinol is widely used in skin anti-aging, yet its effects exhibit significant concentration dependence. However, the underlying biphasic mechanism, that is low concentrations promote cell proliferation...
Discovery of leucine analogues of tyrocidines and tryptocidines from Brevibacillus brevis B011 through genome mining and in vitro biosynthesis
January 2027
Brevibacillus brevis B011 is an endophytic isolate from tobacco roots with significant antifungal activity, yet the metabolites responsible for this phenotype and their biosynthetic mechanisms have...
Reprogramming the enduracidin NRPS assembly line via thioesterase domain relocation
January 2027
The rational engineering of multi-megadalton nonribosomal peptide synthetase (NRPS) assembly lines remains a formidable challenge due to their immense size and complexity. Conventional module deletion...
Improved catalytic efficiency of P450 OleP for converting lithocholic acid into murideoxycholic and ursodeoxycholic acids through semi-rational and rational design
January 2027
Cytochrome P450 enzymes are promising for synthesizing therapeutically beneficial bile acids murideoxycholic acid (MDCA) and ursodeoxycholic acid (UDCA) from lithocholic acid (LCA). However, the catalytic...
Highly efficient production of β-caryophyllene in Saccharomyces cerevisiae using a novel synthase from Artemisia argyi
January 2027
β-Caryophyllene, a bicyclic sesquiterpene shows remarkable application potential in agriculture for plant disease prevention, pest control and green pesticide development. However, conventional production...
Development and characterization of a Cre/loxP toolkit for genome engineering in Komagataella phaffii
January 2027
The Cre/loxP system is a versatile tool for genome engineering, yet its application in the industrial yeast Komagataella phaffii has been limited primarily to marker recycling. This study reports a...
Enhancing myrcene biosynthesis in yeast through nuclear compartmentalization
January 2027
Myrcene is a high-value monoterpene extensively applied in the fragrance, flavor, and agricultural industries, yet its efficient microbial production remains challenging due to pathway competition and...
Identification of integration site library for the development of plasmid-free microbial cell factory in Bacillus subtilis
January 2027
Bacillus subtilis was widely used for enzyme production and was gradually engineered to biosynthesize value-added chemicals with the development of genetic parts for it. Compared to other genetic parts...
Efficient expression of full-length triple-helix recombinant type III collagen in Pichia pastoris
January 2027
Type III collagen (COL3A1) is a significant fibrillar collagen abundant in vascular and cutaneous tissues, playing vital roles in wound healing and tissue repair. The growing demand for COL3A1 in biomedical...
Advancing our understanding of photosynthesis: discoveries and insights from aquatic plants
January 2027
Aquatic plants inhabit highly dynamic environments characterized by rapid and extreme fluctuations in CO2 availability, light intensity, hydrodynamics, temperature, and pH. These conditions have driven...
Deep Learning–driven synergistic engineering of PET hydrolase for post-consumer PET depolymerization
January 2027
Enzymatic depolymerization of polyethylene terephthalate (PET) offers a promising route to mitigate the increasingly severe problem of plastic pollution. However, the development of highly efficient...
Systematic metabolic engineering of Escherichia coli for high-level production of pseudouridine via pathway optimization and precursor enhancement
December 2026
Pseudouridine, the C5-ribose epimer of uridine with significant biological functions and clinical applications, was efficiently produced through systematic metabolic strategy of Escherichia coli in...
Efficient whole-cell biotransformation for gastrodin production via glycosyltransferase engineering and process optimization
December 2026
Gastrodin is a naturally occurring phenolic glycoside derived from Gastrodia elata, which is widely used as an edible medicinal plant in functional foods and dietary supplements in East Asia. Owing...
Two-step bioconversion of pyridoxal 5′-phosphate from pyridoxine through cofactor regeneration and pyridoxine 5′-phosphate oxidase evolution
December 2026
Pyridoxal 5′-phosphate (PLP), a vital cofactor in cellular metabolism, is a high-value compound widely used in the food and pharmaceutical industries. However, the efficient biosynthesis of PLP remains...
Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
December 2026
Xiamenmycin, a prenylated benzopyran compound produced by Streptomyces xiamenensis 318, exhibits promising anti-fibrotic activity but suffers from low productivity, limiting its pharmaceutical development....
Engineering a heme-dependent tryptophan hydroxylase pathway in E. coli for enhanced melatonin production
December 2026
Melatonin is a high-value bioactive indoleamine broadly applied in the pharmaceutical, food and nutraceutical industries, yet its microbial production remains constrained by pathway complexity and low...
Shikimate crosstalk enables record-level homogentisic acid production in Yarrowia lipolytica
December 2026
Homogentisic acid (HGA; 2,5-dihydroxyphenylacetic acid) is an aromatic compound with diverse applications in pharmaceuticals, food, and functional materials. Industrial HGA production still relies mainly...
Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla
December 2026
Paris species are renowned for the production of important medicinal steroidal saponins (e.g., polyphyllins). UDP-dependent glycosyltransferases (UGTs) play important roles in saponin biosynthesis....
CRISPR/Cas9 mediates precise and efficient gene editing in Xanthomonas oryzae pv. oryzae
December 2026
Xanthomonas oryzae pv. oryzae (Xoo), a non-obligate phytopathogen that causes bacterial leaf blight, poses a severe threat to rice production. Although the clustered regularly interspaced short palindromic...
