Precise l-threonine-to-l-isoleucine pathway regulation for engineering high-efficiency whole-cell biocatalysts
June 2026
l-Isoleucine (L-Ile), a critical branched-chain amino acid with diverse applications in food, pharmaceutical, and cosmetic industries, is difficult to produce efficiently at scale in microbial systems...
Truncation of LPD1 promoter and adaptive evolution increase cytosolic acetyl-CoA supply in yeast
June 2026
Acetyl-CoA is a central metabolic intermediate that serves as a key precursor for the biosynthesis of high-value compounds such as terpenoids. However, its compartmentalization within Saccharomyces...
Combinatorial engineering of enzyme and pathway for efficient β-farnesene bioproduction in Yarrowia lipolytica
June 2026
β-farnesene, a natural sesquiterpene compound, has gained significant attention due to its versatile applications in agriculture, industry, biofuels, and related fields. Microbial biosynthesis offers...
Multi-omics elucidation of resource allocation for enhanced ethanol production via precise glucose control in anaerobic Saccharomyces cerevisiae fermentation
June 2026
Ethanol, a high-demand clean energy source, is primarily produced via fed-batch fermentation in industrial settings. Although our previous study identified an optimal glucose concentration of 30 g/L...
High-level production of vitamin K2 in Escherichia coli via modular molecular engineering
June 2026
Menaquinone-7 (MK-7), a key form of vitamin K2 with wide-ranging nutritional and pharmaceutical applications, has attracted increasing interest for microbial production. Here, we developed an integrated...
Engineered dual-fluorescence functional nucleic acid-based CRISPR/Cas12a biosensor for label-free ratiometric detection of site-specific DNA methylation
June 2026
The CRISPR/Cas12a system holds significant promise for biomedical applications. Nevertheless, the commonly used reporter, fluorophore–quencher-labeled substrates, is hindered by labor-intensive synthesis...
Defect-complementation homologous recombination: A novel strategy for precise genome engineering of virulent phages
June 2026
Engineered bacteriophages (phages) have been developed to overcome the limitations of natural phage therapies and serve as precision-targeted agents against drug-resistant bacterial infections. However,...
Combining multiple metabolic strategies for efficient production of longifolene in Saccharomyces cerevisiae
June 2026
Longifolene is a sesquiterpene commonly found in the heavy turpentine oil of pine plants, with various applications ranging from pest control and fragrance production to synthetic biofuels. While S....
Engineering ω-transaminase for efficient dihydroxyacetone transamination in serinol biosynthesis starting from methanol
June 2026
Serinol (2-amino-1,3-propanediol) is an important pharmaceutical intermediate, but conventional chemical or microbial routes are hampered by high energy demand, product toxicity, or complex regulation....
Synthesis of tunable copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate by engineered Halomonas bluephagenesis and their characterizations
June 2026
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with a 0–30 mol% controllable range of 3HV ratios was produced by Halomonas bluephagenesis (H. bluephagenesis) and characterized. An endogenous plasmid...
Programmable large-cargo integration: Overcoming size constraints for next-generation gene therapy
June 2026
The emergence of base and prime editors—genome editing tools that avoid double-strand breaks (DSBs)—has enabled precise point mutations, insertions, inversions, deletions, and substitutions, which accelerates...
Functions and optimization of soft law in the international governance of synthetic biology: The predicament of hard law vs. the rise of soft law
June 2026
Synthetic biology, as an emerging field that integrates life sciences and engineering technology, is driving profound transformations in global science, ethics, and legal systems. In international legal...
Engineering an ATP-saving mevalonate pathway for high-efficiency S-(+)-linalool production in Serratiamarcescens
June 2026
(S)-(+)-linalool, a valuable acyclic monoterpenol secondary metabolite of plants, finds extensive applications in the food, flavor and fragrance, pharmaceutical, and daily chemical industries. Microbial...
Transfer learning with pre-trained language models for protein expression level prediction in Escherichia coli
June 2026
Accurately predicting recombinant protein expression in Escherichia coli remains a long-standing challenge due to the multifactorial nature of gene regulation and translation. Existing computational...
Efficient whole-cell biocatalytic synthesis of 2′-deoxy-2′-fluoroadenosine, a key building block for nucleic acid drugs
June 2026
2′-Deoxy-2′-fluoroadenosine (2′-F-dA) is a nucleoside analogue used as a key building block for oligonucleotide drugs. It can be biosynthesized from a low-cost 2′-deoxy-2′-fluorouridine via one-pot...
Malic enzyme-based system for transhydrogenation between nicotinamide cofactors
March 2026
The nicotinamide cofactors including nicotinamide adenine dinucleotide (NAD) and its phosphate (NADP) play important roles in facilitating redox reactions for energy metabolism and biosynthesis. To...
Topoisomerase II-Like variants enhance acid tolerance and low-pH l-malic acid biosynthesis in metabolic engineered Aspergillus nidulans
March 2026
l-malic acid is a versatile compound having extensive applications across food, pharmaceutical, medical, and chemical industries while its production from microbial fermentation offers sustainable advantages...
Hfq modulates system-wide regulatory networks controlling biocontrol property in Lysobacter enzymogenes
March 2026
Lysobacter enzymogenes is a beneficial soil bacterium renowned for its potent biocontrol properties, primarily attributed to antimicrobial secondary metabolites such as HSAF and WAP-8294A2, as well...
Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production in Yarrowia lipolytica
March 2026
Succinic acid, an essential platform chemical with extensive utility in biodegradable materials, pharmaceuticals, and the food industry, faces challenges of high energy consumption and environmental...
A near-infrared ratio fluorescent probe achieves mitochondrial hydrogen polysulfide imaging for monitoring ferroptosis in arthritis
March 2026
Reactive sulfur (RSS) is a type of sulfur-containing molecule widely present in biological systems. Hydrogen polysulfide (H2Sn, n > 1), as a member of the active sulfur family, plays an indispensable...
Construction and analysis of a cell factory for terpenoid biosynthesis in Pichia pastoris via metabolic engineering and metabolomics
March 2026
Terpenoids are widely distributed in nature and have various applications in health products, pharmaceuticals, and fragrances. Despite the tremendous potential of terpenoids, traditional production...
Enhanced vitamin B6 production in engineered Escherichia coli via restricted mix-carbon feeding and pressure-controlled fermentation
March 2026
Pyridoxine (PN), a major commercial form of vitamin B6, is mainly produced via chemical synthesis, raising environmental concerns. Microbial fermentation offers a greener alternative, but low biomass...
A rapid combinatorial assembly method for gene cluster characterisation illuminates glidobactin biosynthesis
March 2026
Heterologous expression of natural product gene clusters in tractable hosts offers great promise for achieving sustainable production of nature-inspired drugs. However, it is common for the roles of...
Engineering riboflavin-overproducing Bacillus subtilis via pathway gene overexpression
March 2026
Overexpression of genes at key metabolic nodes frequently enhances the yield of target products. However, this often leads to widespread issues with growth defect and plasmid instability. This study...
Efficient trans-aconitic acid production using systematically metabolic engineered Escherichia coli
March 2026
trans-Aconitic acid (TAA) is a versatile platform biochemical exhibiting extensive applications. In this work, Escherichia coli W3110 (DE3) was metabolic modified for the de novo biosynthesis of TAA....