#AI reads Urine# Early changes in urine 1H-NMR metabolomics profile predict cadet’s performance gains after 5 weeks of military training
Published 29 September, 2025
The study employed ¹H-nuclear magnetic resonance (¹H-NMR) metabolomics to analyze urine samples from cadets undergoing a 5-week military training program before the training and during the first week (on days 2 and 7), quantifying 82 urinary metabolites while measuring performance-related variables before and after the training. It identified that early changes in 15 metabolites could significantly predict the cadets' gains in performance indicators such as body mass index, body mass, jump power, jump height, and maximal oxygen uptake (VO₂max) four weeks later. Among these, citrate, 4-pyridoxate, and ascorbate were the most crucial for the predictive models. Except for an increase in trigonelline, the other 14 metabolites showed a significant decrease (50%-90%) compared to pre-training levels. Ultimately, the study confirmed that urinary metabolomics can predict subsequent performance gains by identifying changes in metabolites, and the involved metabolites are mostly related to vitamins, coenzymes, or intermediates of energy metabolism.
Metabolomics. 2025 Sep 26;21(5):145. doi: 10.1007/s11306-025-02348-7.
Youhe Gao
Statement: During the preparation of this work the author(s) used Doubao / AI reading for summarizing the content. After using this tool/service, the author(s) reviewed and edited the content as needed and take(s) full responsibility for the content of the published article.
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