#AI reads Urine# Exploring the Effects of Teas with Different Fermentation Levels and Black Coffee on the Body via the Urine Proteome

This study explored the effects of teas with different fermentation levels (green tea, oolong tea, black tea, Pu-erh tea) and black coffee on rats' bodies through urinary proteomics analysis. Urine samples were collected from rats before and after seven consecutive days of consuming the aforementioned beverages, and analyzed using liquid chromatography-tandem mass spectrometry. The results showed that the urinary proteome could comprehensively and systematically reflect the changes in the rats' bodies after beverage intake, with differential proteins enriched in biological processes and related pathways such as fat cell differentiation, lipid metabolism, glucose metabolism, and immune response. Additionally, the effects of different fermented teas and black coffee on the body were highly specific. Several identified differential proteins have been reported as biomarkers for diseases like cancer and cardiovascular diseases, suggesting that the intake of teas and coffee should be considered in urine biomarker research for diseases, and the use of biomarker panels may be necessary to improve accuracy.

Nutrients 2026, 18, 343；https://www.mdpi.com/2072-6643/18/2/343

Youhe Gao

Statement: During the preparation of this work the author(s) used Doubao / AI reading for summarizing the content. After using this tool/service, the author(s) reviewed and edited the content as needed and take(s) full responsibility for the content of the published article.

For earlier AI Reads Urine articles：

https://www.keaipublishing.com/en/journals/advances-in-biomarker-sciences-and-technology/ai-reads-urine/