#AI reads Urine# Point-of-Care Detection of Urinary N‑Acetyl- β ‑D‑Glucosaminidase
Published 06 January, 2026
This document describes a detection tool called a sequential dual-reagent paper-based analytical device developed by researchers to address the issue that traditional detection methods for N-acetyl-β-D-glucosaminidase in urine— a biomarker that can indicate early renal tubular damage and is of great significance for the early detection and monitoring of chronic kidney disease, especially diabetic nephropathy— rely on complex instrumentation and are not convenient for on-site rapid testing. This device, paired with a portable optical reader, achieves time-sequenced control of the enzymatic reaction and color development process by spatially separating the substrate and alkaline chromogenic reagent required for detection on the test strip and using a mechanically triggered release mechanism. It has a linear detection range of 0-200 U/L, a detection limit of 0.524 U/L, a repeat detection error of less than 6%, good specificity, and a storage stability of 7 days under dry conditions. Additionally, it requires no sample pretreatment, is easy to operate, has a detection time of only 10 minutes, and the test strip cost is low (approximately 2 RMB). Tested with 70 clinical urine samples, the detection results of this device show a correlation of 0.9833 with the standard chemiluminescence detection method used in hospitals, demonstrating good clinical applicability. It is expected to be used for the early screening of populations at high risk of kidney disease (such as diabetic patients) and on-site rapid testing in communities and resource-limited areas, contributing to the early intervention of renal damage and kidney disease management.
ACS Sens. 2025 Nov 24. doi: 10.1021/acssensors.5c02754.
Youhe Gao
Statement: During the preparation of this work the author(s) used Doubao / AI reading for summarizing the content. After using this tool/service, the author(s) reviewed and edited the content as needed and take(s) full responsibility for the content of the published article.
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