Terminal deoxynucleotidyl transferase: Properties and applications
March 2025
Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase, can elongate DNA by adding deoxynucleotides to the 3′ terminal of a DNA chain in a template-independent manner. Owing to their remarkable...
Engineering Saccharomyces cerevisiae for efficient production of recombinant proteins
March 2024
Saccharomyces cerevisiae is an excellent microbial cell factory for producing valuable recombinant proteins because of its fast growth rate, robustness, biosafety, ease of operability via mature genomic...
CRISPR-based gene editing technology and its application in microbial engineering
December 2023
Gene editing technology involves the modification of a specific target gene to obtain a new function or phenotype. Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-mediated...
Carbon sequestration pathways in microorganisms: Advances, strategies, and applications
June 2025
In recent years, industrial activities have significantly increased atmospheric CO2 levels, exacerbating global warming. Carbon reduction involves implementing measures to minimize CO2 emissions from...
Challenges and opportunities for third-generation ethanol production: A critical review
March 2023
In recent decades, third-generation (3G) biofuels have become a more attractive method of fuel production, as algae cultivation does not infringe on resources needed for food production. Additionally,...
The gut virome and human health: From diversity to personalized medicine
March 2025
•The gut virome shapes bacterial populations, metabolism, and immunity, impacting health.•New sequencing technologies have advanced phage-based therapeutics and diagnostics.•Phageomics offers personalized...
Two-component system GacS/GacA, a global response regulator of bacterial physiological behaviors
March 2023
The signal transduction system of microorganisms helps them adapt to changes in their complex living environment. Two-component system (TCS) is a representative signal transduction system that plays...
Complexity of antibiotic resistance and its impact on gut microbiota dynamics
March 2025
The present review explores the influence of the gut microbiota on antibiotic resistance dynamics, particularly those associated with dysbiosis. The improper use of antibiotics can induce resistance...
The emerging role of recombineering in microbiology
September 2023
•Recombineering is a very popular and efficient genetic engineering technique that is based on phage-encoded homologous recombination and functions independently of RecA.•Recombineering is widely used...
Engineered bacteria as drug delivery vehicles: Principles and prospects
September 2022
The development of drug delivery vehicles is in significant demand in the context of precision medicine. With the development of synthetic biology, the use of genetically engineered bacteria as drug...
Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products
June 2022
The mycelial bacterium Streptomyces is a workhorse for producing natural products, serving as a key source of drugs and other valuable chemicals. However, its complicated life cycle, silent biosynthetic...
Genetic tools for metabolic engineering of Pichia pastoris
December 2023
The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is widely used as a yeast cell factory for producing heterologous proteins. Recently, it has gained attention for its potential...
Research advances on the consolidated bioprocessing of lignocellulosic biomass
June 2024
•Lignocellulosic biomass is an abundant resource for the production of bioproducts.•The high cost of cellulase production is the main hurdle for biorefinery industry.•CBP is an alternative approach...
Metabolic engineering using acetate as a promising building block for the production of bio‐based chemicals
December 2022
The production of biofuels and biochemicals derived from microbial fermentation has received a lot of attention and interest in light of concerns about the depletion of fossil fuel resources and climatic...
Engineering of Saccharomyces cerevisiae for co-fermentation of glucose and xylose: Current state and perspectives
September 2023
The use of non-food lignocellulosic biomass to produce ethanol fits into the strategy of a global circular economy with low dependence on fossil energy resources. Xylose is the second most abundant...
Optimizing the CRISPR/Cas9 system for gene editing in Yarrowia lipolytica
June 2025
Yarrowia lipolytica is a promising host for producing valuable chemicals owing to its robustness and metabolic versatility. Efficient genome editing tools are essential for advancing its biotechnological...
CRISPR-Cas9-based genome-editing technologies in engineering bacteria for the production of plant-derived terpenoids
September 2024
Terpenoids are widely used as medicines, flavors, and biofuels. However, the use of these natural products is largely restricted by their low abundance in native plants. Fortunately, heterologous biosynthesis...
Bacterial co-cultivation for the degradation of polystyrene plastics
December 2025
•Microbial co-culture was proposed for the management of polystyrene waste.•The degradation of PS by co-culture was 2.85 times greater than pure culture.•Polystyrene was recycled to muconic acid through...
Systems metabolic engineering of glutathione biosynthesis in Saccharomyces cerevisiae: Pathway balancing coupled with enzyme screening for high-titer production
December 2025
•Novel plasmid-free S. cerevisiae produces 997.46 mg·L⁻¹ glutathione.•Gsh1-Gsh2 fusion resolves γ-glutamylcysteine bottleneck in GSH pathway.•DO-coupled fed-batch boosts GSH titer 2.9 × vs. flasks in...
Advances in synthetic microbial ecosystems approach for studying ecological interactions and their influencing factors
June 2025
Investigating ecological interactions within microbial ecosystems is essential for enhancing our comprehension of key ecological issues, such as community stability, keystone species identification,...
Genetically engineered bacteria as inflammatory bowel disease therapeutics
December 2024
•Bacteria can be genetically modified to produce therapeutic molecules or execute diagnostic functions•Live medicines based on the bacterial chassis represent a breakthrough therapeutic strategy that...
Applications of bacteriophages in precision engineering of the human gut microbiome
March 2025
As our understanding of the role of the gut microbiome in human diseases deepens, precision engineering of the gut microbiome using bacteriophages has gained significant attention. Herein, we review...
Bifidobacterium animalis subsp. lactis genome resources and metabolite profiling at the strain level and their ability to alleviate anxiety-like behavior in a sleep-deprived mouse model
December 2025
Bifidobacterium animalis subsp. lactis is a well-known probiotic with potential benefits for alleviating sub-health symptoms, including immune dysfunction and anxiety. Given the strain-specific nature...
The role of microbiota dysbiosis in Parkinson’s disease: Pathophysiology and therapeutic opportunities
September 2025
Parkinson’s disease (PD) is a chronic, progressive neurodegenerative disorder characterized by debilitating motor and non-motor symptoms. Its etiology is multifactorial, with no single definitive cause...
Recent advances in genome-scale engineering in Escherichia coli and their applications
March 2024
Owing to the rapid advancement of genome engineering technologies, the scale of genome engineering has expanded dramatically. Genome editing has progressed from one genomic alteration at a time that...