Engineering Saccharomyces cerevisiae for efficient production of recombinant proteins
March 2024
Saccharomyces cerevisiae is an excellent microbial cell factory for producing valuable recombinant proteins because of its fast growth rate, robustness, biosafety, ease of operability via mature genomic...
Random mutagenesis platform for accelerated genome evolution (RAMPAGE) of Pichia pastoris
September 2026
The biological conversion of methanol into valuable chemicals represents a promising route for achieving global carbon neutrality. However, the rational engineering of complex industrial traits in microbial...
Exploring the emerging role of CRISPR-Cas systems in probiotic development
September 2026
Modification of the gut microbiota by beneficial microbes can enhance an organism’s lifespan, giving rise to the concept of probiotics. Probiotics are live microorganisms that provide health benefits...
CRISPR-based gene editing technology and its application in microbial engineering
December 2023
Gene editing technology involves the modification of a specific target gene to obtain a new function or phenotype. Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-mediated...
Proteome-informed interrogation of a methanogenic archaeon links stress response mechanisms with microbial performance and robustness during biomethanation
September 2026
•Quantified microbial robustness of Methanothermobacter wolfeii BSEL under bioprocess-relevant perturbations using a Fano-factor metric.•Proteomics revealed stress-specific adaptations involving ribosomal...
Complexity of antibiotic resistance and its impact on gut microbiota dynamics
March 2025
The present review explores the influence of the gut microbiota on antibiotic resistance dynamics, particularly those associated with dysbiosis. The improper use of antibiotics can induce resistance...
Terminal deoxynucleotidyl transferase: Properties and applications
March 2025
Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase, can elongate DNA by adding deoxynucleotides to the 3′ terminal of a DNA chain in a template-independent manner. Owing to their remarkable...
Effect of resistant starch type 5 on gut health through modulating gut microbiota
March 2026
Resistant starch is a dietary fiber that escapes digestion in the small intestine and undergoes fermentation by gut microbiota in the colon, producing beneficial short-chain fatty acids (SCFAs). Among...
Carbon sequestration pathways in microorganisms: Advances, strategies, and applications
June 2025
In recent years, industrial activities have significantly increased atmospheric CO2 levels, exacerbating global warming. Carbon reduction involves implementing measures to minimize CO2 emissions from...
Research advances on the consolidated bioprocessing of lignocellulosic biomass
June 2024
•Lignocellulosic biomass is an abundant resource for the production of bioproducts.•The high cost of cellulase production is the main hurdle for biorefinery industry.•CBP is an alternative approach...
Systematic optimization of Komagataella phaffii (Pichia pastoris) for high-efficiency recombinant protein production: Advancements in strain engineering, fermentation optimization, and AI-driven technologies
December 2026
Komagataella phaffii (formerly Pichia pastoris) has emerged as an indispensable eukaryotic expression host for the production of industrial enzymes, food proteins, and therapeutic proteins owing to...
Systematic engineering to enhance 3-carene production in yeast
September 2026
•The NPP orthogonal pathway was introduced, and the well-matched key enzyme of SsCarTS was selected and truncated to increase the yield of 3-carene.•The appropriate subcellular localization into endoplasmic...
SuSha: A multi-model ensemble learning framework for predicting microbial salinity adaptation
September 2026
Current research on microbial salinity adaptation faces substantial challenges, including the limited predictive accuracy of traditional single-gene models and difficulty in dissecting systemic biological...
Optimizing the CRISPR/Cas9 system for gene editing in Yarrowia lipolytica
June 2025
Yarrowia lipolytica is a promising host for producing valuable chemicals owing to its robustness and metabolic versatility. Efficient genome editing tools are essential for advancing its biotechnological...
Two-component system GacS/GacA, a global response regulator of bacterial physiological behaviors
March 2023
The signal transduction system of microorganisms helps them adapt to changes in their complex living environment. Two-component system (TCS) is a representative signal transduction system that plays...
Genetic tools for metabolic engineering of Pichia pastoris
December 2023
The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is widely used as a yeast cell factory for producing heterologous proteins. Recently, it has gained attention for its potential...
Engineering of Saccharomyces cerevisiae for co-fermentation of glucose and xylose: Current state and perspectives
September 2023
The use of non-food lignocellulosic biomass to produce ethanol fits into the strategy of a global circular economy with low dependence on fossil energy resources. Xylose is the second most abundant...
Prospects and challenges in using engineered lactic acid bacteria in aquaculture applications
June 2026
Despite the considerable potential of lactic acid bacteria (LAB) as probiotics, there is a fundamental gap between the functional limitations of wild-type strains and the complex demands of aquaculture....
When phage restriction is not immunity: Immune gates, replication-niche barriers, and assay-limited phenotypes
December 2026
Antiphage discovery increasingly yields restrictive phenotypes before mechanisms are known. This Perspective argues that reduced plaque formation or low efficiency of plating should be classified phenotype-first,...
Structure-guided surface engineering to improve the catalytic activity of ethylene-forming enzyme
September 2026
The ethylene-forming enzyme (EFE) is a member of the mononuclear non-heme Fe(II)- and 2-oxoglutarate-dependent oxygenase superfamily, which can oxidize 2-oxoglutarate to form ethylene in an arginine-dependent...
Metabolic engineering using acetate as a promising building block for the production of bio‐based chemicals
December 2022
The production of biofuels and biochemicals derived from microbial fermentation has received a lot of attention and interest in light of concerns about the depletion of fossil fuel resources and climatic...
Advances in polyethylene biodegradation and bioconversion: Microbial, enzymatic, and biotechnological insights
March 2026
Polyethylene (PE) is one of the most widely used plastics worldwide and is valued for its versatility, durability, and cost-effectiveness. However, the chemical stability of PE combined with its widespread...
An efficient endogenous type I-E CRISPR-Cas genome-editing platform for producing transglutaminase in Streptomyces mobaraensis
December 2026
Streptomyces mobaraensis is an industrially important actinomycete capable of producing transglutaminase (TGase), a valuable crosslinking enzyme that is widely used in the food, pharmaceutical, and...
Herbal extracts as antibiotic adjuvants against ESKAPE pathogens: Mechanisms and therapeutic potential
June 2026
•Herbal extracts act as antibiotic adjuvants against ESKAPE pathogens.•Synergistic herbal-antibiotic effects arise from multi-target mechanisms.•AI accelerates the effective discovery of antibiotic...
Identification of a functional DddX-like enzyme that catalyzes dimethylsulfoniopropionate cleavage in marine bacteria
December 2026
•A novel DddX-like DMSP lyase was identified from the marine Roseobacter group.•The enzyme shares ∼23% identity with DddX and forms a distinct clade.•The DddX-like enzyme catalyzes DMSP cleavage to...