CRISPR-based gene editing technology and its application in microbial engineering
December 2023
Gene editing technology involves the modification of a specific target gene to obtain a new function or phenotype. Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-mediated...
Engineering Saccharomyces cerevisiae for efficient production of recombinant proteins
March 2024
Saccharomyces cerevisiae is an excellent microbial cell factory for producing valuable recombinant proteins because of its fast growth rate, robustness, biosafety, ease of operability via mature genomic...
Terminal deoxynucleotidyl transferase: Properties and applications
March 2025
Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase, can elongate DNA by adding deoxynucleotides to the 3′ terminal of a DNA chain in a template-independent manner. Owing to their remarkable...
Carbon sequestration pathways in microorganisms: Advances, strategies, and applications
June 2025
In recent years, industrial activities have significantly increased atmospheric CO2 levels, exacerbating global warming. Carbon reduction involves implementing measures to minimize CO2 emissions from...
Two-component system GacS/GacA, a global response regulator of bacterial physiological behaviors
March 2023
The signal transduction system of microorganisms helps them adapt to changes in their complex living environment. Two-component system (TCS) is a representative signal transduction system that plays...
Genetically encoded biosensors enabled high-throughput screening of microbial cell factories
March 2026
•Genetically encoded biosensors couple product synthesis to detectable HTS outputs.•Synthetic biology and AI-driven strategies accelerate precise biosensor engineering.•Optimization protocols are detailed...
The gut virome and human health: From diversity to personalized medicine
March 2025
•The gut virome shapes bacterial populations, metabolism, and immunity, impacting health.•New sequencing technologies have advanced phage-based therapeutics and diagnostics.•Phageomics offers personalized...
Complexity of antibiotic resistance and its impact on gut microbiota dynamics
March 2025
The present review explores the influence of the gut microbiota on antibiotic resistance dynamics, particularly those associated with dysbiosis. The improper use of antibiotics can induce resistance...
Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products
June 2022
The mycelial bacterium Streptomyces is a workhorse for producing natural products, serving as a key source of drugs and other valuable chemicals. However, its complicated life cycle, silent biosynthetic...
Genetic tools for metabolic engineering of Pichia pastoris
December 2023
The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is widely used as a yeast cell factory for producing heterologous proteins. Recently, it has gained attention for its potential...
Research advances on the consolidated bioprocessing of lignocellulosic biomass
June 2024
•Lignocellulosic biomass is an abundant resource for the production of bioproducts.•The high cost of cellulase production is the main hurdle for biorefinery industry.•CBP is an alternative approach...
Engineering of Saccharomyces cerevisiae for co-fermentation of glucose and xylose: Current state and perspectives
September 2023
The use of non-food lignocellulosic biomass to produce ethanol fits into the strategy of a global circular economy with low dependence on fossil energy resources. Xylose is the second most abundant...
Metabolic engineering using acetate as a promising building block for the production of bio‐based chemicals
December 2022
The production of biofuels and biochemicals derived from microbial fermentation has received a lot of attention and interest in light of concerns about the depletion of fossil fuel resources and climatic...
Pathway reprogramming and catalytic network engineering for the production of bioactive aspertetranones from deep-sea Aspergillus versicolor ADS-F20
June 2026
Aspertetranones are a unique class of marine fungal meroditerpenoids characterized by a highly oxygenated, linear 6/6/6/6 tetracyclic core fused to an α-pyrone scaffold. Although the pathway of aspertetranone...
Effect of resistant starch type 5 on gut health through modulating gut microbiota
March 2026
Resistant starch is a dietary fiber that escapes digestion in the small intestine and undergoes fermentation by gut microbiota in the colon, producing beneficial short-chain fatty acids (SCFAs). Among...
Engineered bacteria as drug delivery vehicles: Principles and prospects
September 2022
The development of drug delivery vehicles is in significant demand in the context of precision medicine. With the development of synthetic biology, the use of genetically engineered bacteria as drug...
Advances in polyethylene biodegradation and bioconversion: Microbial, enzymatic, and biotechnological insights
March 2026
Polyethylene (PE) is one of the most widely used plastics worldwide and is valued for its versatility, durability, and cost-effectiveness. However, the chemical stability of PE combined with its widespread...
Applications of bacteriophages in precision engineering of the human gut microbiome
March 2025
As our understanding of the role of the gut microbiome in human diseases deepens, precision engineering of the gut microbiome using bacteriophages has gained significant attention. Herein, we review...
Advances in the dynamic control of metabolic pathways in Saccharomyces cerevisiae
December 2023
The metabolic engineering of Saccharomyces cerevisiae has great potential for enhancing the production of high-value chemicals and recombinant proteins. Recent studies have demonstrated the effectiveness...
Optimizing the CRISPR/Cas9 system for gene editing in Yarrowia lipolytica
June 2025
Yarrowia lipolytica is a promising host for producing valuable chemicals owing to its robustness and metabolic versatility. Efficient genome editing tools are essential for advancing its biotechnological...
Genetically engineered bacteria as inflammatory bowel disease therapeutics
December 2024
•Bacteria can be genetically modified to produce therapeutic molecules or execute diagnostic functions•Live medicines based on the bacterial chassis represent a breakthrough therapeutic strategy that...
CRISPR-Cas9-based genome-editing technologies in engineering bacteria for the production of plant-derived terpenoids
September 2024
Terpenoids are widely used as medicines, flavors, and biofuels. However, the use of these natural products is largely restricted by their low abundance in native plants. Fortunately, heterologous biosynthesis...
Challenges and opportunities for third-generation ethanol production: A critical review
March 2023
In recent decades, third-generation (3G) biofuels have become a more attractive method of fuel production, as algae cultivation does not infringe on resources needed for food production. Additionally,...
Advances in synthetic microbial ecosystems approach for studying ecological interactions and their influencing factors
June 2025
Investigating ecological interactions within microbial ecosystems is essential for enhancing our comprehension of key ecological issues, such as community stability, keystone species identification,...
Recent advances in genome-scale engineering in Escherichia coli and their applications
March 2024
Owing to the rapid advancement of genome engineering technologies, the scale of genome engineering has expanded dramatically. Genome editing has progressed from one genomic alteration at a time that...