Adherence to biosafety and biosecurity protocols: An assessment of biomedical and veterinary laboratories in Benin

Constrained by insufficient funding and incomplete regulatory frameworks, most African nations suffer poor biosafety systems. In particular, Benin scored merely 25.4% in the 2021 Global HealthSecurity Index, lacking official biosafety evaluation benchmarks, which necessitated this field investigation.

Funded by the U.S. Department of State, a cross-sectional survey assessed 96 laboratories across Benin in November 2023, including 93 biomedical and three veterinary facilities. The researchers adopted the BSSLAT and FAO's LMT assessment tools respectively, collected data via KoBoCollect and performed statistical analysis using R software. 

“The overall biosafety risk was alarming: 42% of surveyed labs fell under very high risk, 38% high risk, 15% moderate risk and only 5% low risk,” says the study's corresponding author, Philippe Sessou.

In particular, 80% of biomedical laboratories and all veterinary ones were categorized as high-risk sites. These labs routinely store hazardous pathogens ranging from malaria parasites, hepatitis viruses and tuberculosis bacilli to Ebola and Lassa viruses, yet inadequate facilities and flawed management fail effective pathogen containment.

The team of researchers found only two assessment modules of biomedical labs (premise layout and disinfection/waste disposal) met international standards. “Severe deficiencies existed in staff training, standard experimental practices, emergency preparation and biosecurity management,” says Sessou. “Most labs had no dedicated biosafety officers or standardized operating manuals, with shortages of biosafety cabinets, emergency eyewash stations and designated handwashing sinks.” 

Loopholes were also found in pathogen registration, access control and digital data protection. All three veterinary laboratories failed compliance checks; though basic access restriction was enforced, they suffered from uninspected key equipment, insufficient emergency supplies and improper waste disposal.

“Consistent with related research in African settings, Benin’s poor biosafety status stems from outdated infrastructure, insufficient staff training, incomplete national regulations and chronic financial shortage,” adds Sessou.

The team's recommendations include enacting localized biosafety codes and regular tiered training for practitioners. “The Beninese government ought to boost financial input to renovate lab infrastructure, supplement safety equipment and recruit full-time biosafety specialists,” urges Sessou. “In addition, international exchanges can help introduce mature biosafety governance experience.”

The study's findings are published in the Journal of Biosafety and Biosecurity.

Contact the author:

Philippe Sessou, Research Unit of Communicable Diseases, Laboratory of Research in Applied Biology, Ecole Polytechnic d'Abomey-Calavi, University of Abomey-Calavi, Benin. philippe.sessou@uac.bj

Funder:

This research was supported by the Elizabeth R. Griffin Program through funding provided by the U.S. Department of State, Nonproliferation and Disarmament Fund (grant number SISNDF22GR0010). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Conflict of interest:

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

See the article:

Philippe Sessou, Nestor Noudeke, Yves Eric Denon, Aretas Tonouhewa, Alban Zohoun, Nassirou Boukary, Désirée Metodakou, Brian Samuelson, Dana Hag Hamed, Jenny Zhao, Sumegha Asthana, Mohammed Abid, Erin M. Sorrell. Adherence to biosafety and biosecurity protocols: An assessment of biomedical and veterinary laboratories in Benin. Journal of Biosafety and Biosecurity. Volume 7, Issue 1, 2025, Pages 28-37. https://doi.org/10.1016/j.jobb.2024.11.001.