Advances in synthetic microbial ecosystems approach for studying ecological interactions and their influencing factors
June 2025
Investigating ecological interactions within microbial ecosystems is essential for enhancing our comprehension of key ecological issues, such as community stability, keystone species identification,...
Modification of essential factors mediating post-translational processing for high-quality protein expression in Penicillium
March 2025
The formation of mature proteins requires complex post-translational modification and processing. Efficient post-translational processing machinery is beneficial for the high-quality expression of proteins....
Applications of bacteriophages in precision engineering of the human gut microbiome
March 2025
As our understanding of the role of the gut microbiome in human diseases deepens, precision engineering of the gut microbiome using bacteriophages has gained significant attention. Herein, we review...
Characterization of a small non-coding RNA S612 in Bacillus subtilis
March 2025
Small regulatory RNAs (sRNAs) are non-coding RNA molecules that fine-tune various cellular processes and respond to various environmental stimuli. In Bacillus subtilis, the regulatory mechanisms and...
Terminal deoxynucleotidyl transferase: Properties and applications
March 2025
Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase, can elongate DNA by adding deoxynucleotides to the 3′ terminal of a DNA chain in a template-independent manner. Owing to their remarkable...
Complexity of antibiotic resistance and its impact on gut microbiota dynamics
March 2025
The present review explores the influence of the gut microbiota on antibiotic resistance dynamics, particularly those associated with dysbiosis. The improper use of antibiotics can induce resistance...
BTG13-related metalloenzymes: Atypical non-heme iron-dependent dioxygenases with unusual coordination patterns and catalytic mechanisms
March 2025
Owing to their diverse coordination patterns and catalytic mechanisms, non-heme iron-dependent dioxygenases catalyze a variety of biochemical reactions involved in the synthesis of numerous natural...
Activating cryptic biosynthetic gene clusters via ACTIMOT
March 2025
The mainstream strategy of genome mining relies on the homologous activation and heterologous expression of target biosynthetic gene clusters (BGCs). However, the efficiency of the current techniques...
Establishment and improvement of genetic manipulation tools for Fusobacterium nucleatum
March 2025
•Suicide-plasmid-based homologous recombination enables precise target gene inactivation and complementation in Fusobacterium nucleatum.•The controllable expression system or CRISPRi system carried...
The gut virome and human health: From diversity to personalized medicine
March 2025
•The gut virome shapes bacterial populations, metabolism, and immunity, impacting health.•New sequencing technologies have advanced phage-based therapeutics and diagnostics.•Phageomics offers personalized...
Proactive monitoring of changes in the microbial community structure in wastewater treatment bioreactors using phospholipid fatty acid analysis
December 2024
Diverse microbial community structures (MCS) in wastewater treatment plants (WWTPs) are vital for effectively removing nutrients and chemicals from wastewater. However, the regular monitoring of MCS...
Immobilization of Thermomyces lanuginosus lipase on metal-organic frameworks and investigation of their catalytic properties and stability
December 2024
Surface adsorption is a convenient and readily available method for immobilizing enzymes on metal-organic frameworks (MOFs). Metal-organic framework-5 (MOF-5), isoreticular metal-organic frameworks-3...
The way to uncovering and utilizing marine microbial resources
December 2024
Recently, Chen et al. published their breakthrough results on a marine microbial genomic catalog and genetic potentials in bioprospecting in Nature, providing unprecedented opportunities for development...
Key amino acid residues govern the substrate selectivity of the transporter Xltr1p from Trichoderma reesei for glucose, mannose, and galactose
December 2024
•The Q291A mutant of Xltr1p accelerated the utilization of mannose as compared to the wild-type transporter.•The Y301W mutant lost the galactose utilization capacity but retained glucose utilization...
Exploring interspecific interaction variability in microbiota: A review
December 2024
Interspecific interactions are an important component and a strong selective force in microbial communities. Over the past few decades, there has been a growing awareness of the variability in microbial...
Genetically engineered bacteria as inflammatory bowel disease therapeutics
December 2024
•Bacteria can be genetically modified to produce therapeutic molecules or execute diagnostic functions•Live medicines based on the bacterial chassis represent a breakthrough therapeutic strategy that...
Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439
December 2024
Cytochrome P450 enzymes (CYPs or P450s) and ferredoxins (Fdxs) are ubiquitously distributed in all domains of life. Bacterial P450s are capable of catalyzing various oxidative reactions with two electrons...
Biofuel production from lignocellulose via thermophile-based consolidated bioprocessing
December 2024
•Thermophile-based consolidated bioprocessing is the potential way to produce biofuels from biomass.•Thermophile-based CBP offers the potential to simplify operation processes.•Synthetic biology strategy...
Mechanisms of tigecycline resistance in Gram-negative bacteria: A narrative review
September 2024
Tigecycline serves as a critical “final-resort” antibiotic for treating bacterial infections caused by multidrug-resistant bacteria for which treatment options are severely limited. The increasing prevalence...
CRISPR-Cas9-based genome-editing technologies in engineering bacteria for the production of plant-derived terpenoids
September 2024
Terpenoids are widely used as medicines, flavors, and biofuels. However, the use of these natural products is largely restricted by their low abundance in native plants. Fortunately, heterologous biosynthesis...
Cellulolytic characterization of the rumen-isolated Acinetobacter pittii ROBY and design of a potential controlled-release drug delivery system
September 2024
•A novel cellulose degrading strain ROBY was isolated from a bovine rumen.•Strain identity of ROBY was found to be Acinetobacter pittii.•Degradation by ROBY was assumed to be aerobic due to the nature...
Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines
September 2024
•This research has successfully devised a technique to lower the denaturation temperature of the crucial SARS-CoV-2 protein on purpose by modifying specific amino acids within its structural core.•The...
Dual role of phage terminase in Salmonella enterica oxidative stress response
September 2024
The adaptive survival mechanisms of bacterial pathogens under host-induced stress are crucial for understanding pathogenesis. Recently, Uppalapati et al. revealed a unique dual function of the Gifsy-1...