Monitoring abandoned cropland in the hilly and gully regions of the Loess Plateau using Landsat time series images
November 2025
•Two distinct change detection methods were proposed to monitor abandoned cropland using Landsat images in the hilly and gully regions.•The land-use trajectory method identified abandoned cropland more...
Changes in cropland soil inorganic carbon and its relationship with nitrogen fertilization and precipitation over the past 40 years in the Sichuan Basin, China
November 2025
•Machine learning models were developed to fill the missing soil inorganic carbon (SIC) of soil samples.•Cropland soils experienced an overall decrease in SIC during the 1980s–2010s.•Precipitation was...
Glycosylphosphatidylinositol (GPI) anchoring controls cell wall integrity, immune evasion and surface localization of ChFEM1 for infection of Cochlibolus heterostrophus
November 2025
•GPI anchoring is indispensable for cell-wall integrity and full virulence of the maize pathogen Cochliobolus heterostrophus.•Deletion of ChGPI7 or ChFEM1 crippled appressorium formation, exposes chitin,...
Baseline establishment, susceptibility monitoring and risk assessment of cyproflanilide, a novel meta-diamide insecticide, against Chilo suppressalis (Lepidoptera: Crambidae) in China
November 2025
•The baseline of susceptibility of Chilo suppressalis to cyproflanilide was established as LD50=0.122 ng/larva by topical application and LC50=0.026 mg L–1 by the rice-seedling dipping method.•The realized...
Fhb9, a major QTL for Fusarium head blight resistance improvement in wheat
November 2025
•Qfhb-2DL was identified by evaluating a recombinant inbred line (RIL) population derived from Shi4185×Shijiazhuang 8, similar to the previously reported position in Ji5265 and designated Fhb9.•Fhb9...
Genome-wide identification of the PpSWEET gene family and function characterization of PpSWEET6 associated with endodormancy release in peach bud
November 2025
•Fifteen peach SWEET family members were identified, and the expression patterns of the PpSWEET genes varied during peach bud dormancy.•PpSWEET6 expression increased substantially during peach bud endodormancy...
A one-pot Cas12i3/Cas13d-based assay for rapid portable identification of genotypes I and II African swine fever viruses
November 2025
•A one-pot Cas12i3/Cas13d-based assay is developed to discriminate between ASFV genotype I and II.•This method demonstrates complete concordance with the PCR-sequencing method within 45 min....
The glycoside hydrolase 7 member VdGH7a regulates Verticillium dahliae pathogenicity and induces host defenses by interacting with GhOLP1
November 2025
•Activation of the plant immune system by VdGH7a.•V. dahliae's pathogenicity toward cotton has been positively regulated by VdGH7a.•VdGH7a interacted with GhOLP1 in vitro and in vivo tests.•Cotton's...
TbNACα negatively regulates Trichoderma breve T069 synthesis of ethyl caffeate and enhances antagonism of Sclerotium rolfsii
November 2025
•In Trichoderma breve T069, the non-volatile metabolites of ΔTbNACα mutant strongly inhibited the growth of Sclerotium rolfsii.•Deletion of the TbNACα increases the accumulation of ethyl caffeate, thereby...
Establishment and analysis of a chicken skeletal muscle satellite cell line using TERT
November 2025
•Lentiviral infection of chTERT successfully immortalized chicken skeletal muscle satellite cell lines.•These cell lines retained the primary cells’ proliferative and differentiation capacities without...
Streptococcus suis serotype 2 collagenase-like protease promotes meningitis by increasing blood–brain barrier permeability
November 2025
•Clp enhances the toxic effect of SS2.•Clp increases the permeability of the blood–brain barrier by disrupting tight junctions and inducing apoptosis of brain endothelial cells.•Clp triggers brain endothelial...
Functional characterization and identification of superior haplotypes of barley HvGL7-2H (Hordeum vulgare L.) in grain features
November 2025
•HvGL7-2H gene serves a crucial function in barley grain length development.•Superior haplotypes of HvGL7-2H can be used in barley molecular breeding....
Identification and transfer of resistance to Fusarium head blight from Elymus repens chromosome arm 7StL into wheat
November 2025
•Development and identification of a new wheat–Elymus repens 7DS·7StL translocation line with Fusarium head blight (FHB) resistance.•Elymus repens chromosome arm 7StL carries the dominant FHB resistance...
Natural variation in SbTEF1 contributes to salt tolerance in sorghum seedlings
November 2025
•The sorghum salt tolerant gene SbTEF1 was identified by genome-wide association study (GWAS) and the PAV284 which located in the promoter region of SbTEF1 was the key regulatory locus governing the...
Mutation in CmGhc1 confers the white hypocotyl phenotype in melon (Cucumis melo L.)
November 2025
•The white hypocotyl trait in melon was controlled by a single recessive gene, designated CmGhc1.•A single-base deletion in the fifth exon of CmGhc1 led to a truncated CmGhc1 lacking the HTH-MYB DNA...
Genome-wide association studies of novel resilience traits identify important immune QTL regions and candidate genes in Duroc pigs
November 2025
•Four novel resilience traits for pigs were developed from daily feed intake and feeding duration data.•Twenty-seven loci associated with resilience traits were identified in Duroc pigs and mapped to...
Long-term excessive nitrogen application decreases spring maize nitrogen use efficiency via suppressing root physiological characteristics
November 2025
•Long-term excessive nitrogen application promoted morphological characteristics, but suppressed physiological characteristics of maize root.•Excessive nitrogen application did not enhance post-silking...
Optimizing rice yield: Evaluating the nitrogen supply characteristics of slow- and controlled-release fertilizers using the leaf nitrogen balance index
November 2025
•The study developed a novel diagnostic approach using the leaf nitrogen balance index to evaluate the nitrogen supply characteristics of slow- and controlled-release nitrogen fertilizers (SCRNFs).•Integration...
Unlocking Dendrobium officinale's drought resistance: Insights from transcriptomic analysis and enhanced drought tolerance in tomato
November 2025
•Dendrobiumofficinale demonstrated survival through 6 months of water deficit and exhibited rapid growth recovery within 2 d after rehydration.•Drought tolerance mechanisms in D. officinale encompass...
Mapping soil organic carbon in fragmented agricultural landscapes: The efficacy and interpretability of multi-category remote sensing variables
November 2025
•Multi-category remote sensing variables can improve our understanding of soil organic carbon (SOC) variation in fragmented agricultural landscapes.•Spectral transformations significantly contribute...
No-tillage with total green manure mulching increases maize yield through improved soil moisture and temperature environment and enhanced maize root structure and photosynthetic capacity
November 2025
•No-tillage with total green manure (GM) mulching (NTG) can optimize maize root structure by improving soil water content (SWC) and soil temperature (ST) environment.•NTG increased maize photosynthetic...
Haplotype-resolved telomere-to-telomere reference genome of sweet cherry 'Tieton’ v3.0 characterized the large fragment deletion associated with yellow-skinned variety
November 2025
•This study presents the assembly of a haplotype-resolved telomere-to-telomere reference genome of sweet cherry 'Tieton’ v3.0.•The sweet cherry 'Tieton’ v3.0 reference genome enabled the characterization...
A transient transformation method for pre-screening gRNAs in CRISPR/Cas gene editing
November 2025
•This method rapidly evaluates the efficiency of gRNA sequences for genome editing.•This method confirms the indicator effect of transient conversion on stable transformations.•This approach reduces...
Temperament and production in ruminants: The microbiome as one of the factors that affect temperament
November 2025
•Temperament modifies the responses to stressors and impacts the production capacity and welfare of ruminants.•While molecular studies have identified central pathways that shape temperament, emerging...
Estimation model of potassium content in cotton leaves based on hyperspectral information of multi-leaf position
November 2025
•Vertical distribution characteristics of leaf potassium content (LKC, %) of cotton have been clarified.•Considering the instability and limited applicability of the single-leaf position and layered-leaf...