Effect of nitrogen fertilizer on proso millet starch structure, pasting, and rheological properties
July 2025
•N application reduced amylose content and increased crystallinity and lamellar thickness.•N deteriorated the shear resistance and storage capacity of proso millet (PM) starch during processing.•PM...
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Identification and molecular marker development for peel color gene in melon (Cucumis melo L.)
July 2025
•The dark green peel trait at maturity of the near-isogenic line NIL-G derived from B8 (Yangjiaocui) and B15 (Yiwohou) was controlled by a single dominant gene.•The alternative splicing of the CmAPRR2...
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NAC family gene CmNAC34 positively regulates fruit ripening through interaction with CmNAC-NOR in Cucumis melo
July 2025
•The physicochemical properties, molecular features, and expression profiles of the NAC transcription factor family members in melon were elucidated.•Genetic transformation showed that CmNAC34 and CmNAC-NOR...
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Genetic analysis of maize crude fat content by multi-locus genome-wide association study
July 2025
•The genetic architecture of crude fat content in maize kernels was studied by genome-wide association studies using six models.•A total of 744 significant quantitative trait nucleotides (QTNs) and...
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Preparation and characterization of monoclonal antibodies against the pp62 protein of African swine fever virus
June 2025
•Two mAbs (4B5-19-7 and 4C5-11-6) specifically recognizing the ASFV polyprotein pp62 were prepared, which can further recognize its mature proteins p15 and p35, respectively.•Heavy-chain CDR sequences...
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Introgressed eggplant lines with the wild Solanum incanum evaluated under drought stress conditions
June 2025
•Introgression of Solanum incanum genomic regions improves drought tolerance in eggplant.•Solanum incanum introgressions on chromosomes 1, 2, 6, 8, and 12 increase water use efficiency (WUE), leaf hydration,...
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Overexpression of TuABCC4 is associated with abamectin resistance in Tetranychus urticae Koch
June 2025
•A novel ATP-binding cassette (ABC) transporter gene (TuABCC4) was cloned in Tetranychus urticae.•TuABCC4 expression levels were significantly increased in the abamectin-resistant populations.•TuABCC4...
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Evolution and biological characteristics of the circulated H8N4 avian influenza viruses
June 2025
•The systematic analysis of the available data enhances our comprehension of the spatial and temporal distribution of H8 subtype avian influenza viruses.•The genetic diversity of avian influenza viruses...
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PGC-mediated conservation strategies for germplasm resources of Rugao Yellow chicken and Shouguang chicken in China
June 2025
•The study successfully cultured primordial germ cells (PGCs) from Chinese indigenous chickens (e.g., Rugao Yellow chicken and Shouguang chicken) with a culture efficiency of 39.1–45%.•These cells retained...
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A suitable organic fertilizer substitution ratio stabilizes rainfed maize yields and reduces gaseous nitrogen loss in the Loess Plateau, China
June 2025
•The optimal substitution (37.5%) of inorganic fertilizer with organic fertilizer promoted maize production and reduced gaseous nitrogen loss in northern China rainfed maize fields.•NH4+-N, alkali-hydrolyzed...
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The circadian clock shapes diurnal gene expression patterns linked to glucose metabolic processes in Chinese cabbage
June 2025
•The circadian clock broadly regulates gene expression in Chinese cabbage, with gene expression peaks occurring earlier in short-period lines compared to long-period lines.•Circadian-controlled alternative...
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The N-mannosyltransferase MoAlg9 plays important roles in the development and pathogenicity of Magnaporthe oryzae
June 2025
•MoAlg9 localizes to endoplasmic reticulum (ER) and plays critical roles in cell morphogenesis and pathogenesis in rice blast fungus.•The transcription levels of genes related to conidiation, appressorium...
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QTL detection and candidate gene analysis of the anthracnose resistance locus in tea plant (Camellia sinensis)
June 2025
•Anthracnose resistance QTLs in tea plants were investigated using the BJG×LJ43 population.•Two anthracnose resistance QTLs, qARChr1 and qARChr13, were identified through BSA-seq analysis.•A nuclear-localized...
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Identification of oil content QTLs on Arahy12 and Arahy16, and development of KASP markers in cultivated peanut (Arachis hypogaea L.)
June 2025
•Two major effect QTLs for oil content on Arahy16 and Arahy12 were identified and diagnostic KASP markers were developed.•The superior haplotype associated with high oil content on Arahy16 is primarily...
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ORF138 causes abnormal lipid metabolism in the tapetum that leads to Ogu cytoplasmic male sterility in Brassica napus
June 2025
•Confirmed orf138 as the causal gene for Ogu cytoplasmic male sterility through genetic transformation.•Differentially expressed genes (DEGs) in aborted anthers are mainly enriched in glycerophospholipid...
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GWAS analysis reveals candidate genes associated with density tolerance (ear leaf structure) in maize (Zea mays L.)
June 2025
•Genome-wide association analysis was performed to identify new loci for density tolerance (ear leaf structure) in maize.•The gene Zm00001d008651 regulating maize density tolerance by ear and leaf structure...
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Long-term Chinese milk vetch incorporation promotes soil aggregate stability by affecting mineralogy and organic carbon
June 2025
•Aggregate stability is higher in high-clay Udic Ferrisols than in low-clay Alfisols.•Kaolinite, Fed, and aromatic-C drive clay formation, raising aggregate stability.•36-year milk etch (MV) incorporation...
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MdERF2 regulates cuticle wax formation by directly activating MdLACS2, MdCER1 and MdCER6 of apple fruit during postharvest
June 2025
•MdERF2 positively regulated MdLACS2, MdCER1, MdCER4 and MdCER6 expression levels.•MdERF2 directly binds to the promoters of the MdLACS2, MdCER1 and MdCER6 genes.•MdERF2 altered apple fruit cuticular...
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The estimation method is the primary source of uncertainty in cropland nitrate leaching estimates in China
June 2025
•Different combinations (N inputs×Areas×Methods) resulted in estimates ranging from 2.9 to 15.8 Tg N yr−1, with a mean of 6.7 Tg N yr−1.•Estimation method was the primary contributor to uncertainty...
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SiDWARF4 encodes an ent-copalyl diphosphate synthase (CPS) required for gibberellin biosynthesis and morphogenesis in foxtail millet
June 2025
•Phenotype of dwarf4 is due to the abnormal intersegmental elongation and reduced gibberellin synthesis.•SiDWARF4 encodes the ent-copalyl diphosphate synthase (CPS) enzyme involved in gibberellin (GA)...
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Molecular evidence of the west-to-east dispersal of Puccinia striiformis f. sp. tritici in central Shaanxi and the migration of the inoculum from Gansu
June 2025
•Inoculum of Puccinia striiformis f. sp. tritici (Pst) causing wheat stripe rust in central Shaanxi are mainly originated from Gansu, especially Longnan and Tianshui.•Molecular evidences support the...
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Transcriptome-based analysis of lignin accumulation in the regulation of fruit stone development and endocarp hardening in Chinese jujube
June 2025
•RNA-Seq analysis of three jujube cultivars across key endocarp developmental stages revealed dynamic gene expression networks critical for regulating stone formation in Chinese jujube.•Lignin accumulation...
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Post-anthesis dry matter production and leaf nitrogen distribution are associated with root-derived cytokinins gradient in rice
June 2025
•A steeper nitrogen (N) distribution can enhance N utilization efficiency and dry matter production in rice.•Such N distribution is driven by a larger gradient of zeatin (Z) and zeatin riboside (ZR)...
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The coupled effects of various irrigation schedules and split nitrogen fertilization modes on post-anthesis grain weight variation, yield, and grain quality of drip-irrigated winter wheat (Triticum aestivum L.) in the North China Plain
June 2025
•Water-nitrogen management for wheat grain development was assessed.•A 50–50 basal-topdressing drip fertigation enhanced yield and grain quality.•A 50–50 basal-topdressing drip fertigation promoted...
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Protection of chickens from Mycoplasma gallisepticum through the MAPK/ERK/JNK pathway by a compound of ten Chinese medicine formulas
June 2025
•TCM improved body weight gain, feed conversion, survival rate, and immune organ index in MG-infected chickens.•TCM reduced MG-induced inflammation and tissue damage by lowering pro-inflammatory cytokines...
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