HiBiT-tagged influenza A virus: A stable and efficient tool for antiviral reagent screening and vaccine evaluation
October 2025
•The reporter H9N2 virus containing HiBiT tag derived from NanoLuc luciferase was viable and genetically stable.•H9N2-HiBiT virus provided a highly efficient quantitative tool for screening antiviral...
Integrated transcriptomic and metabolomic analyses reveal the mechanisms for red fruit coloration in peach cv. ‘Zhongyoupan 9’ under artificial darkness
October 2025
•The research demonstrated that ‘Zhongyoupan 9’ peach fruits displayed light-independent anthocyanin biosynthesis under artificial darkness.•PpHY5 exhibited upregulation, and its expression pattern...
Dehydrin PbDHN3 regulates ethylene synthesis and signal transduction to improve salt tolerance in pear
October 2025
•PbDHN3 enhanced salt tolerance in pear.•PbDHN3 precisely regulated ethylene synthesis and signaling transduction, thereby enhancing salt tolerance in plants.•OE-PbDHN3 pear plants exhibited enhanced...
Multiple chromosomal configurations and phylogenetic implications in Saccharum mitochondrial genomes
October 2025
•Mitochondria integrally influence plant growth, fertility and adaptation.•Multiple chromosomal configurations are present in Saccharum complex mitogenomes.•There are substantial genomic reorganization...
Identification of novel QTLs for resistance to late leaf spot in peanut by SNP array and QTL-seq analyses
October 2025
•An environmental stable major quantitative trait locus (QTL) conferring resistance to late leaf spot disease (LLS) was identified on chromosome (Chr) 02 from peanut germplasm Mi-2.•A structural variation...
Optimizing tillage and fertilization practices to improve the carbon footprint and energy efficiency of wheat–maize cropping systems
October 2025
•Subsoiling rotary tillage (SRT) optimizes C footprint (CF) and energy use.•Hybrid fertilizer (HF) reduces CF and energy efficiency.•SRT+HF has the best overall performance for crop production, CF and...
Abscisic acid reduces Cd accumulation by regulating Cd transport and cell wall sequestration in rice
October 2025
•Numerous abscisic acid (ABA)-related genes are responsive to cadmium (Cd) stress in rice plants.•ABA reduces Cd accumulation by suppressing Cd uptake and regulating cell wall sequestration.•Foliar...
TBX3 orchestrates H3K4 trimethylation for porcine induced pluripotent stem cells to totipotent-like stem cells
October 2025
•TBX3 reprograms porcine iPSCs into totipotent-like 4-cell stage cells.•TBX3 activates MLL1 to enhance H3K4me3 modification in porcine iPSCs....
Methyl salicylate reduces aphid abundance in maize through multiple modes of action
October 2025
•Methyl salicylate effectively deters both wingless and winged aphids by disrupting their ability to colonize and infest maize plants.•Methyl salicylate can attract predatory natural enemies of aphids.•Exposure...
circKIF27 inhibits melanogenesis and proliferation by targeting miR-129-5p/TGIF2 pathway in goat melanocytes
October 2025
•circKIF27 acts as a negative regulator of cell proliferation and melanogenesis by targeting miR-129-5p expression in goat melanocytes.•miR-129-5p inhibits the expression of TGIF2 in goat by directly...
Convergent and divergent signaling pathways in C3 rice and C4 foxtail millet crops in response to salt stress
October 2025
•Rice and foxtail millet exhibit different physiological and genetic responses to salt stress.•The miR172-AP2/ERF signaling module is highly conserved under salt stress in rice and foxtail millet....
Response of fresh maize yield to nitrogen application rates and characteristics of nitrogen-efficient varieties
October 2025
•Sweet maize yield relies more on kernel number, whereas waxy maize depends more on kernel weight under low nitrogen but on both traits under high nitrogen.•Greater plant or ear heights and optimal...
Quantitative assessment of the transport and distribution of photosynthetic carbon and exogenous nitrogen in a rice–soil system under long-term straw return: An isotope trial using 13C and 15N labelling
October 2025
•The transport and distribution of 13C and 15N under rice straw return (SR) is clarified.•SR improves the partitioning of 13C and 15N in rice plants.•SR significantly improves photosynthetic C transfer...
Combining GWAS and RNA-seq approaches identifies the FtADH1 gene for drought resistance in Tartary buckwheat
October 2025
•FtADH1, identified via membership function value of drought tolerance (MFVD), genome-wide association study (GWAS), and transcriptomics, enhances drought resistance by promoting root growth and reactive...
Functional insights into the FW2.2-like gene family in Chinese jujube: Identification, characterization, and impact of ZjFWL10 variants on fruit size and plant height
October 2025
•A 7-bp deletion in the PLAC8 domain of ZjFWL10 is significantly associated with reduced jujube fruit size.•ZjFWL10 overexpression in tomato suppresses fruit size and increases plant height....
Quantifying the contribution of triple compound extreme events to global yield loss of major staple crops from 1982 to 2016
October 2025
•Assessed global crop yield responses to individual and compound extremes using a linear mixed-effects model from 1982 to 2016.•Compound events (HWLP and HDW) caused significantly greater yield losses...
Involvement of FoVEL1 and FoLAE1 in conidiation, virulence and secondary metabolism of Fusarium oxysporum f. sp. niveum
October 2025
•The VEL1 and LAE1 genes play a role in determining the virulence of Fusarium oxysporum f. sp. niveum.•Yeast two-hybrid demonstrated an interaction between FoVel1 and FoLae1 in Fusarium oxysporum f....
Optimization of fertilization combined with water-saving irrigation improves the water and nitrogen utilization efficiency of wheat and reduces nitrogen loss in the Nansi Lake basin, China
October 2025
•Four water and fertilizer regimes validate the WHCNS model’s accuracy.•F2W2 notably reduces nitrogen loss while maintaining a stable yield.•F2W2 reduces nitrogen leaching and ammonia volatilization...
Rapid improvement of rice appearance quality by targeted knockout of the GS9 gene
October 2025
•Most japonica rice varieties possess similar alleles for grain size, producing short and wide grains.•The gs9ko allele has a widely applicable effect on grain appearance improvement.•The increased...
Trichoderma gamsii strain TC959 with comprehensive functions to effectively reduce seedling damping-off and promote growth of pepper by direct and indirect action mechanisms
October 2025
•Trichoderma gamsii strain TC959 is discovered possessing comprehensive capabilities in terms of strong antagonistic and plant growth-promoting properties.•The strain directly inhibits plant pathogen...
CRISPR/xCas9-mediated corazonin knockout reveals the effectiveness of xCas9 editing and the crucial role of corazonin in insect cuticle development
October 2025
•xCas9 shows effective gene-editing capabilities at target sites with a non-canonical AG PAM.•xCas9 can cleave target sites with canonical NGG PAMs, but with lower activity than SpCas9.•Corazonin–/–...
Immunogenicity and efficacy of an LNP-mRNA prepared from African swine fever virus K205R
October 2025
•This study provides that an mRNA vaccine targeting the K205R antigen can induce antibody production in both mice and pigs.•Pigs vaccinated with the K205R mRNA showed a significantly delayed time to...
Integrated transcriptomic and metabolomic analyses reveal a novel mechanism of resistance to Colletotrichum fructicola in pear
October 2025
•Lipid metabolism is critically associated with anthracnose resistance in ‘Seli’ pear.•Dysregulated biosynthesis of amino acids and abnormal metabolism of proanthocyanidins contribute to anthracnose...
Development and application of a double-antigen Sandwich ELISA using capsid protein to detect multispecies antibodies against porcine circovirus type 3
October 2025
•A novel double antigen sandwich ELISA (DAgS-ELISA) using baculovirus-expressed PCV3 Cap protein was established, enabling sensitive detection of PCV3 antibodies across diverse animal species.•A serosurvey...
Soybean variety influences the advantages of nutrient uptake and yield in soybean/maize intercropping via regulating root–root interaction and rhizobacterial composition
October 2025
•The effects of soybean varieties BD2 and YC03-3 on plant N and P uptake, growth, yield and the rhizosphere microorganism community were compared in a maize/soybean intercropping system•BD2/maize intercropping...