Innovative methods for isolating highly purified mitochondria essential for biomedical studies
2024
Mitochondria, being multifunctional and highly complex organelles, possess unique structures and exhibit heterogeneity. In recent decades, the isolation and purification of functional mitochondria have...
A protocol for detecting the cytoplasm-exposed mitochondrial DNA
2024
Mitochondria, being semi-autonomous organelles, possess a double membrane structure and harbor their own DNA, known as mtDNA. In situations of stress, mtDNA is released from the mitochondrial membrane...
The mitochondria chronicles of melatonin and ATP: Guardians of phase separation
2024
Phase separation is a thermodynamic process used by all living organisms since the origin of life to rapidly assemble and disassemble membraneless condensates in response to changes in exogenous and...
The metabolism and function of phospholipids in Mitochondria
2023
Mitochondria are important organelles of eukaryotic cells and involved in a variety of cellular processes including oxidative phosphorylation for generating ATP, apoptosis, steroid synthesis, and signal...
Mitochondrial Dynamics Regulators in Cancer Metabolism and Progression
Available online 19 April 2025
Mitochondrial dynamics, encompassing fusion and fission processes, are crucial to cancer’s metabolic adaptation and progression. This review explores the intricate relationship between mitochondrial...
Extra-mitochondrial ATP synthesis, proton dynamics at the membrane, and mitochondria-derived vesicles: Current findings and considerations
2025
Adenosine triphosphate (ATP) is essential for almost all life forms. In our article, we discuss (i) insights into the bioenergetics of ATP generation, including an extended view of proton currents during...
Single-cell mitochondrial DNA sequencing: Methodologies and applications
2024
Mitochondria play a pivotal role in cellular energetics, metabolism, and various regulatory processes. Their dysregulation is implicated in numerous diseases. Traditional population-level mitochondrial...
Mitochondrial‐derived vesicles: at the crossroads of mitochondrial functions and metabolites
2025
•Fumarate buildup drives MDV-mediated mtDNA release, sparking cGAS-STING inflammation.•BHB induces SNX9 Kbhb, promoting MDVs biogenesis to regulate mitochondrial function.•MDVs show dual roles: inflammatory...
Photobleaching and phototoxicity of mitochondria in live cell fluorescent super-resolution microscopy
2024
Photobleaching and phototoxicity can induce detrimental effects on cell viability and compromise the integrity of collected data, particularly in studies utilizing super-resolution microscopes. Given...
Decoding the influence of mitochondrial Ca2+ regulation on neurodegenerative disease progression
2025
Mitochondria are pivotal hubs in maintaining cellular homeostasis, encompassing vital processes such as bioenergetics, redox regulation, Ca2+ signaling, and programmed cell death. Ca2+ is a key second...
Are patients with mitochondrial diseases prone to inflammatory and immune dysfunction: A scoping review and retrospective chart analysis
2025
Mitochondrial diseases (MDs) are a significant patient burden and are linked to the dysregulation of various metabolic processes and cellular energy production. Additionally, mitochondria play a central...
Antioxidants targeting mitochondria function in kidney diseases
2024
Kidney diseases are a growing health problem worldwide, causing millions of deaths. Acute kidney injury (AKI) commonly evolves into chronic kidney disease (CKD) and fibrosis, which is a feature of CKD...
Structural determinants of mitochondrial STAT3 targeting and function
2024
Signal transducer and activator of transcription (STAT) 3 has been found within mitochondria in addition to its canonical role of shuttling between cytoplasm and nucleus during cytokine signaling. Mitochondrial...
Lipid transfer at mitochondrial membrane contact sites
2024
Mitochondria are semiautonomous organelles that are actively shaped into an extended reticular network that is not connected to the endomembrane system via vesicular transport. Instead, active lipid...
Sex differences in the genetics of autism spectrum disorder and their impact on gene expression associated with mitochondrial function
2024
Autism spectrum disorder (ASD) is a neurodevelopmental condition that affects social-communicative skills and is characterized by restricted and stereotyped behaviors. It affects more males, with a...
In vivo imaging of neuronal mitochondrial Ca2+ transients with two-photon microscopy in awake mice
2025
Mitochondrial Ca2+ plays important roles in shaping intracellular Ca2+ signaling and modulating energy metabolism. Dysregulated mitochondrial Ca2+ dynamics have been increasingly implicated in the pathogenesis...
Mitochondrial calcium transport during autophagy initiation
2024
While it has been shown that Ca2+ dynamics at the ER membrane is essential for the initiation of certain types of autophagy such as starvation-induced autophagy, how mitochondrial Ca2+ transport changes...
Exploring the oncogenic impact of heteroplasmic de novo MT-ND5 truncating mutations
2025
Numerous mitochondrial DNA (mtDNA) variants are associated with cancers, yet the causal link remains inconclusive. Using DddA-derived cytosine base editors, we induced de novo truncating mutations in...
Is localized chemiosmosis necessary in mitochondria? Is Lee's TELP protonic capacitor hypothesis a reasonable model?
2024
Recent high-resolution pH measurements in mitochondria show ΔpH across the F1F0 ATP synthase to be quite low, 0.07–0.32. Our meta-analysis of published values of transmembrane potential (Δψ) shows it...
Mitochondrial lipid metabolism in metastatic breast cancer
2024
The significance of mitochondrial lipid metabolism in cancer stemness, survival, and proliferation, particularly in the context of metastasis, has garnered significant attention. Warburg's hypothesis...
A brief introduction to the history of mitochondrial research in Japan
2023
The Japanese Society for Mitochondrial Research and Medicine (J-mit) has a 21-year history in 2022, with its predecessor society holding its first meeting in 2001. During this time, the society, which...
Correcting abnormal mitochondrial dynamics to facilitate tumor treatment
2023
Mitochondrial dynamics are closely related to various cellular physiological activities, including cell proliferation, homeostasis, and cell migration, and are regulated by a variety of enzymes, proteins...
Age-related dynamics of m.189A>G and m.408T>A variants in skeletal muscle of an osteoarthritic Cohort: Connections to BMI and muscle strength
2025
The origin and expansion of mitochondrial somatic variants, influenced by tissue-specific mutagenesis and selection, are not well understood despite their relevance to aging and age-related diseases....
Role of TFEB and TFE3 in mediating lysosomal and mitochondrial adaptations to contractile activity in skeletal muscle myotubes
2023
Exercise is potent stimulus for mitochondrial adaptations, serving to activate mitochondrial biogenesis as well as mitochondrial turnover. Through the process of mitophagy, dysfunctional mitochondria...
