Innovative methods for isolating highly purified mitochondria essential for biomedical studies
2024
Mitochondria, being multifunctional and highly complex organelles, possess unique structures and exhibit heterogeneity. In recent decades, the isolation and purification of functional mitochondria have...
The mitochondria chronicles of melatonin and ATP: Guardians of phase separation
2024
Phase separation is a thermodynamic process used by all living organisms since the origin of life to rapidly assemble and disassemble membraneless condensates in response to changes in exogenous and...
A protocol for detecting the cytoplasm-exposed mitochondrial DNA
2024
Mitochondria, being semi-autonomous organelles, possess a double membrane structure and harbor their own DNA, known as mtDNA. In situations of stress, mtDNA is released from the mitochondrial membrane...
The metabolism and function of phospholipids in Mitochondria
2023
Mitochondria are important organelles of eukaryotic cells and involved in a variety of cellular processes including oxidative phosphorylation for generating ATP, apoptosis, steroid synthesis, and signal...
Single-cell mitochondrial DNA sequencing: Methodologies and applications
2024
Mitochondria play a pivotal role in cellular energetics, metabolism, and various regulatory processes. Their dysregulation is implicated in numerous diseases. Traditional population-level mitochondrial...
Extra-mitochondrial ATP synthesis, proton dynamics at the membrane, and mitochondria-derived vesicles: Current findings and considerations
2025
Adenosine triphosphate (ATP) is essential for almost all life forms. In our article, we discuss (i) insights into the bioenergetics of ATP generation, including an extended view of proton currents during...
Are patients with mitochondrial diseases prone to inflammatory and immune dysfunction: A scoping review and retrospective chart analysis
2025
Mitochondrial diseases (MDs) are a significant patient burden and are linked to the dysregulation of various metabolic processes and cellular energy production. Additionally, mitochondria play a central...
In vivo imaging of neuronal mitochondrial Ca2+ transients with two-photon microscopy in awake mice
2025
Mitochondrial Ca2+ plays important roles in shaping intracellular Ca2+ signaling and modulating energy metabolism. Dysregulated mitochondrial Ca2+ dynamics have been increasingly implicated in the pathogenesis...
Decoding the influence of mitochondrial Ca2+ regulation on neurodegenerative disease progression
2025
Mitochondria are pivotal hubs in maintaining cellular homeostasis, encompassing vital processes such as bioenergetics, redox regulation, Ca2+ signaling, and programmed cell death. Ca2+ is a key second...
Antioxidants targeting mitochondria function in kidney diseases
2024
Kidney diseases are a growing health problem worldwide, causing millions of deaths. Acute kidney injury (AKI) commonly evolves into chronic kidney disease (CKD) and fibrosis, which is a feature of CKD...
Photobleaching and phototoxicity of mitochondria in live cell fluorescent super-resolution microscopy
2024
Photobleaching and phototoxicity can induce detrimental effects on cell viability and compromise the integrity of collected data, particularly in studies utilizing super-resolution microscopes. Given...
Lipid transfer at mitochondrial membrane contact sites
2024
Mitochondria are semiautonomous organelles that are actively shaped into an extended reticular network that is not connected to the endomembrane system via vesicular transport. Instead, active lipid...
Off-target effects of mitochondrial oxidative phosphorylation inhibitors are common and can compromise the viability of cultured cells
2025
Mitochondrial respiratory chain inhibitors (MRCIs) are indispensable for studying cellular bioenergetics and its effects on various cellular processes. However, their off-target (those not mediated...
A chemical-genetic approach to target voltage-sensitive fluorophores to mitochondria
2025
Mitochondria play central roles in the physiology of eukaryotic cells. Mitochondrial membrane potential, in turn, is a key driver of mitochondrial physiology. We previously developed a system to localize...
Sex differences in the genetics of autism spectrum disorder and their impact on gene expression associated with mitochondrial function
2024
Autism spectrum disorder (ASD) is a neurodevelopmental condition that affects social-communicative skills and is characterized by restricted and stereotyped behaviors. It affects more males, with a...
Frontiers in LONP1 research: Unraveling molecular mechanisms, disease associations, and therapeutic strategies
2025
LONP1 is a conserved mitochondrial AAA + protease central to mitochondrial proteostasis. This review summarizes its structural features, protease-chaperone functions, and roles in metabolic regulation...
Mitochondrial‐derived vesicles: at the crossroads of mitochondrial functions and metabolites
2025
•Fumarate buildup drives MDV-mediated mtDNA release, sparking cGAS-STING inflammation.•BHB induces SNX9 Kbhb, promoting MDVs biogenesis to regulate mitochondrial function.•MDVs show dual roles: inflammatory...
Mitochondrial NMNAT3 reduces amyloid aggregates in Drosophila and alters the amyloid processing in the 3xTgAD mouse
2026
Alzheimer's disease is a progressive neurological disorder characterized by two main neuropathological hallmarks: neurofibrillary tangles and amyloid plaques. Both are protein aggregates, composed mainly...
Is localized chemiosmosis necessary in mitochondria? Is Lee's TELP protonic capacitor hypothesis a reasonable model?
2024
Recent high-resolution pH measurements in mitochondria show ΔpH across the F1F0 ATP synthase to be quite low, 0.07–0.32. Our meta-analysis of published values of transmembrane potential (Δψ) shows it...
Exploring the oncogenic impact of heteroplasmic de novo MT-ND5 truncating mutations
2025
Numerous mitochondrial DNA (mtDNA) variants are associated with cancers, yet the causal link remains inconclusive. Using DddA-derived cytosine base editors, we induced de novo truncating mutations in...
