Rapidly generating homozygous mutate zebrafish in F0 generation by technical integration of CRISPR/Cas9 and gynogenesis
June 2023
Gene editing technique has been widely applied for gene function characterization. However, such an approach is often time-consuming to obtain homozygous mutant. In this article, through timing the...
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Proximate compositions evaluation, histology and transcriptome analysis revealed the effects of formulated diets on muscle quality in Micropterus salmoides
June 2023
With the gradually upgrade of formulated feeds, the largemouth bass Micropterus salmoides has been domesticated to adapt to formulated diets to reduce the use of bait fish and cut cost. However, whether...
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Role of the dimerization domain of black carp STING during the antiviral innate immunity
June 2023
Stimulator of interferon gene (STING) functions importantly as antiviral adaptor protein during the innate immune activation. The role of the dimerization domain (DD) of STING remains obscure although...
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Generating tetraploid zebrafish by heat shock treatment and labeling microtubules of their cells in vitro
June 2023
Tetraploid is a very important experimental material, whether in scientific research or breeding practice. However, tetraploid strains are relatively rare. It was reported that chromosomes allocation...
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The formation and study of allogynogenesis Hemibarbus maculatus Bleeker
March 2023
Hemibarbus maculatus Bleeker (HM) is an economically important fish that is highly valued by consumers. Gynogenesis is an important breeding method in aquaculture that has been widely applied to many...
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Gynogenetic Cirrhinus mrigala produced using irradiated sperm of Cyprinus carpio exhibit better cold tolerance
March 2023
Artificial gynogenesis is an important method for accelerating the selective breeding of varieties and populations. In this study, ultraviolet-irradiated sperm of Common carp (Cyprinus carpio) was used...
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Air-assisted liquid-liquid microextraction and enantioselective gas chromatography-tandem mass spectrometry quantification of methyl jasmonate stereoisomers in tea (Camellia sinensis L.)
March 2023
Different stereoisomers of phytohormones may have different roles in plants while the quantification for stereoisomers still remains a challenge. The chiral separation method for methyl jasmonate (MeJA)...
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Protection and utilization status of Parabramis and Megalobrama germplasm resources
March 2023
The genera Parabramis and Megalobrama are important freshwater aquaculture economic fish, and the excavation and utilization of excellent germplasm resources are pivotal in ensuring the supply of high-quality...
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Genetic parameter estimates for litter traits in Jakhrana breed of Indian goat
March 2023
The litter size and litter weight are the most important indicators of growth production system in goats as they are directly related to the survival and growth of the kids. The present attempt was...
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The infection of murine gammaherpesvirus-68 delays the early embryonic development of zebrafish
March 2023
Zebrafish is a model animal for aquaculture as well as bio-medicine. Murine gamma-herpesvirus 68 (MHV68) is widely regarded as a model for the study of human gamma herpesviruses such as Kaposi's sarcoma-associated...
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CrRLK1L receptor kinases-regulated pollen-pistil interactions
December 2022
Mutual communications and precise recognitions between the male side pollen and female side pistil are crucial for successful fertilization. Catharanthus roseus receptor-like kinases (CrRLK1Ls) participate...
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Apical vesicles: Social networking at the pollen tube tip
December 2022
Pollen tube growth is asymmetric and highly focused at the tip. It is regarded as an ideal model cell system in plants to study polar cell growth and cell polarity. The rapid pollen tube growth is empowered...
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The OsNramp4 aluminum transporter is involved in cadmium accumulation in rice grains
December 2022
Cadmium (Cd) toxicity affects many crops. Members of the OsNramp (Natural resistance-associated macrophage protein) gene family play important roles in the transport of divalent or trivalent cations...
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Adaptation and integration of environmental cues to internal flowering network in Arabidopsis thaliana
December 2022
Transition to flowering is timed by environmental cues in plants, which makes plants flower at a favorable time for seed setting. Seasonal cues like cold exposure and day length and environmental abiotic...
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Screening of antagonistic bacteria against Flavobacterium columnus and its effects on growth performance and immune function of Carassius auratus
December 2022
With the popularization of modern intensive farming models, aquatic animals are more prone to outbreaks of various diseases. Due to the abuse of antibiotics, drug resistance among pathogenic bacteria...
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Comparative analysis of the texture, composition, antioxidant capacity and nutrients of natural gynogenesis blunt snout bream and its parent muscle
December 2022
Gynogenesis, as a special method of distant hybridization, can be used to accelerate the formation of breeding lines such as fish varieties and populations, and cultivate lines or varieties with higher...
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Comparative analysis of muscle nutrient in two types of hybrid bream and native bream
September 2022
Distant hybridization can integrate parental advantages to produce new varieties, which may also improve the fish meat quality. Fish is considered to be one of the healthiest meats because it can provide...
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A new type of hybrid golden pompano “Chenhai No.1” produced by the hybridization of (Trachinotus ovatus ♀ × Trachinotus blochii ♂) ♀ × T. ovatus ♂
September 2022
Distant hybridization is a traditional and effective technology in fish genetic breeding. In this study, we reported a new type of golden pompano (Named as Chenhai No.1) produced by the distant hybridization...
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Exogenous paternal mitochondria rescue hybrid incompatibility and the destiny of exogenous mitochondria
September 2022
The mitochondria of most organisms follow strict maternal inheritance, and the mechanism of elimination of paternal mitochondria is unclear. Our previous studies showed that the paternal mtDNA presented...
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Androgen receptor α peak expression in retina rather than gonad of Hainan medaka, Oryzias curvinotus
September 2022
Androgen is a member of the sex steroid hormones family which plays an important role in the development and maturation of the vertebrate reproductive system. The physiological function of androgens...
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The isolation of a novel Streptomyces termitum and identification its active substance against fish pathogens
September 2022
The emerging outbreak of bacterial diseases poses a major threat to the aquaculture industry, and the abuse of antibiotics leads to the emergence of drug-resistant pathogens; hence, the development...
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Rapid establishment of Oct4: EGFP transgenic zebrafish homozygote through gynogenesis for monitoring the pluripotency during induction of pluripotent stem cells
September 2022
Oct4 was one of the important markers of cellular pluripotency. In this study, by Tol2 transgenic technology, the recombinant plasmids composed of Oct4 promoter DNA and enhanced green fluorescent protein...
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Culture conditions for in vitro maturation of oocytes – A review
June 2022
The maturation of oocytes external to the body is referred to IVM and is utilised by many different species. IVM bypasses traditional superovulation methods, where several hormonal injections are administered....
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Duplicated Tyr disruption using CRISPR/Cas9 reveals melanophore formation in Oujiang color common carp (Cyprinus carpio var. color)
June 2022
Gene duplication plays an important role in the regulation of biological traits. The Tyr inactivation have been confirmed to hinder melanin synthesis, but the research of duplicated Tyr function and...
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OsFH13, a type I formin, is indispensable for rice morphogenesis
June 2022
Actin binding proteins regulate plant morphogenesis by regulating actin dynamics and formins affect plant morphogenesis by modulating cytoskeleton dynamics. However, the roles of formins in rice morphogenesis...
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