DNA storage: The future direction for medical cold data storage
June 2025
DNA storage, characterized by its durability, data density, and cost-effectiveness, is a promising solution for managing the increasing data volumes in healthcare....
Engineering Rubisco to enhance CO2 utilization
March 2024
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a pivotal enzyme that mediates the fixation of CO2. As the most abundant protein on earth, Rubisco has a significant impact on global carbon,...
Engineered microorganisms: A new direction in kidney stone prevention and treatment
June 2024
Numerous studies have shown that intestinal and urinary tract flora are closely related to the formation of kidney stones. The removal of probiotics represented by lactic acid bacteria and the colonization...
Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering
September 2025
Astaxanthin is a natural red carotenoid, commonly used as an additive in the pharmaceutical industry and as a nutritional supplement owing to its notable antioxidant benefits. However, a complex biosynthetic...
Advances in adaptive laboratory evolution applications for Escherichia coli
December 2025
Adaptive Laboratory Evolution (ALE), a well-established framework in microbial evolution research, is widely applied in synthetic biology. By simulating natural selection through controlled serial culturing,...
Integrating chemistry knowledge in large language models via prompt engineering
March 2025
This paper presents a study on the integration of domain-specific knowledge in prompt engineering to enhance the performance of large language models (LLMs) in scientific domains. The proposed domain-knowledge...
Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production
June 2025
Corynebacterium glutamicum is a versatile industrial microorganism for producing various amino acids. However, there have been no reports of well-defined C. glutamicum strains capable of hyperproducing...
Ergothioneine biosynthesis: The present state and future prospect
March 2025
Ergothioneine (ERG), a rare natural thio-histidine derivative with potent antioxidant properties and diverse biological functions, is widely utilized in food processing, cosmetics, pharmaceuticals,...
Metabolic engineering of Escherichia coli for squalene overproduction
December 2025
Squalene, a lipophilic triterpene with multifaceted bioactivities, faces bioproduction bottlenecks in microbial hosts due to inefficient biosynthetic pathways and limited storage capacity. Here, we...
Melatonin biosynthesis pathways in nature and its production in engineered microorganisms
March 2022
Melatonin is a biogenic amine that can be found in plants, animals and microorganism. The metabolic pathway of melatonin is different in various organisms, and biosynthetic endogenous melatonin acts...
SemiSynBio: A new era for neuromorphic computing
September 2024
Neuromorphic computing has the potential to achieve the requirements of the next-generation artificial intelligence (AI) systems, due to its advantages of adaptive learning and parallel computing. Meanwhile,...
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis
June 2023
Bacillus licheniformis is one of the most characteristic Gram-positive bacteria. Its unique genetic background and safety characteristics make it have important biologic applications in the food industry,...
A systematic discussion and comparison of the construction methods of synthetic microbial community
December 2024
Synthetic microbial community has widely concerned in the fields of agriculture, food and environment over the past few years. However, there is little consensus on the method to synthetic microbial...
Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesis
September 2023
N-Acetylneuraminic acid (Neu5Ac), the most common type of Sia, generally acts as the terminal sugar in cell surface glycans, glycoconjugates, oligosaccharides, lipo-oligosaccharides, and polysaccharides,...
Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production in Yarrowia lipolytica
March 2026
Succinic acid, an essential platform chemical with extensive utility in biodegradable materials, pharmaceuticals, and the food industry, faces challenges of high energy consumption and environmental...
CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris
June 2025
The important methylotrophic yeast Pichia pastoris has been utilized for the production of a variety of heterologous recombinant proteins and has great potential for use in the production of value-added...
Synthetic polycistronic sequences in eukaryotes
December 2021
The need for co-ordinate, high-level, and stable expression of multiple genes is essential for the engineering of biosynthetic circuits and metabolic pathways. This work outlines the functionality and...
Engineering Yarrowia lipolytica for high-level β-elemene production via combinatorial metabolic strategies
December 2025
β-Elemene, a pharmacologically active sesquiterpene derived from the traditional Chinese medicinal herb Curcuma wenyujin, exhibits broad-spectrum anti-tumor and anti-inflammatory activities. Currently,...
Complex peptide natural products: Biosynthetic principles, challenges and opportunities for pathway engineering
March 2022
Complex peptide natural products exhibit diverse biological functions and a wide range of physico-chemical properties. As a result, many peptides have entered the clinics for various applications. Two...
Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials
September 2021
Bacillus subtilis is a commonly used commercial specie with broad applications in the fields of bioengineering and biotechnology. B. subtilis is capable of producing both biofilms and spores. Biofilms...
Discovery and modification of cytochrome P450 for plant natural products biosynthesis
September 2020
Cytochrome P450s are widespread in nature and play key roles in the diversification and functional modification of plant natural products. Over the last few years, there has been remarkable progress...
Combination of enzyme engineering and quorum sensing system for efficient de novo biosynthesis of β-arbutin in Escherichia coli
March 2026
β-Arbutin is a plant-derived glycoside with various biological activities, which has been applied in pharmaceuticals and whitening cosmetics. Uneven carbon flux distribution and the low catalytic efficiency...
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation
September 2022
Cytochrome P450 enzymes (CYPs) catalyze a series of C–H and C=C oxygenation reactions, including hydroxylation, epoxidation, and ketonization. They are attractive biocatalysts because of their ability...
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies
June 2022
The global market demand for natural astaxanthin is rapidly increasing owing to its safety, the potential health benefits, and the diverse applications in food and pharmaceutical industries. The major...
Machine learning-assisted rational design and evolution of novel signal peptides in Yarrowia lipolytica
December 2025
Microbial proteins hold great promise as sustainable alternatives for future protein sources, and oleaginous yeast Yarrowia lipolytica has emerged as a recognized platform for heterologous protein expression...