Systematic metabolic engineering of Escherichia coli for high-level production of pseudouridine via pathway optimization and precursor enhancement
December 2026
Pseudouridine, the C5-ribose epimer of uridine with significant biological functions and clinical applications, was efficiently produced through systematic metabolic strategy of Escherichia coli in...
Efficient whole-cell biotransformation for gastrodin production via glycosyltransferase engineering and process optimization
December 2026
Gastrodin is a naturally occurring phenolic glycoside derived from Gastrodia elata, which is widely used as an edible medicinal plant in functional foods and dietary supplements in East Asia. Owing...
Two-step bioconversion of pyridoxal 5′-phosphate from pyridoxine through cofactor regeneration and pyridoxine 5′-phosphate oxidase evolution
December 2026
Pyridoxal 5′-phosphate (PLP), a vital cofactor in cellular metabolism, is a high-value compound widely used in the food and pharmaceutical industries. However, the efficient biosynthesis of PLP remains...
iFLinkC-EZ: A scalable and automatable method for the assembly of complex fusion proteins and multi-gene expression constructs based on the iFLinkC framework
December 2026
Standardized methods for the assembly of DNA constructs have become indispensable in synthetic biology, biotechnology and basic research. The majority of frameworks—most prominently based on Golden...
Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
December 2026
Xiamenmycin, a prenylated benzopyran compound produced by Streptomyces xiamenensis 318, exhibits promising anti-fibrotic activity but suffers from low productivity, limiting its pharmaceutical development....
Synergistic engineering of monoterpenoid synthase and metabolic pathways to enhance α-terpineol production in Saccharomyces cerevisiae
December 2026
Plant-derived monoterpenoid α-terpineol has gained significant attention due to its versatile applications in the food, cosmetic and pharmaceutical industries. Microbial biosynthesis offers an environmentally...
Engineering a heme-dependent tryptophan hydroxylase pathway in E. coli for enhanced melatonin production
December 2026
Melatonin is a high-value bioactive indoleamine broadly applied in the pharmaceutical, food and nutraceutical industries, yet its microbial production remains constrained by pathway complexity and low...
Shikimate crosstalk enables record-level homogentisic acid production in Yarrowia lipolytica
December 2026
Homogentisic acid (HGA; 2,5-dihydroxyphenylacetic acid) is an aromatic compound with diverse applications in pharmaceuticals, food, and functional materials. Industrial HGA production still relies mainly...
Modulating ilvA encoding threonine deaminase for balanced growth and PHB synthesis by Halomonas grown in rich nitrogen source
December 2026
Bioplastic poly(3-hydroxybutyrate) (PHB) production by Halomonas bluephagenesis is typically activated under nitrogen limitation, inevitably restricting biomass formation and overall productivity. Here...
Discovery of functional compounds from dimorphic edible Ophiocordyceps sinensis fungus in host haemolymph
December 2026
Chinese cordyceps, with a fruiting body of Ophiocordyceps sinensis from insect cadaver, is well-known health food. During formation, O. sinensis experiences a dimorphic transition from blastospores...
Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla
December 2026
Paris species are renowned for the production of important medicinal steroidal saponins (e.g., polyphyllins). UDP-dependent glycosyltransferases (UGTs) play important roles in saponin biosynthesis....
Iterative evolution of Saccharomyces cerevisiae enhances recombinant protease secretion
December 2026
Efficient secretion of heterologous proteases in S. cerevisiae remains a major bottleneck due to host cytotoxicity and secretory stress. In this study, we developed a yeast chassis strain through three...
Constructing an efficient synthesis pathway in peroxisomes to boost β-myrcene production in yeast
December 2026
β-Myrcene is a key acyclic monoterpene that has been extensively applied in the agricultural pesticide, fragrance, and flavor industries; it also serves as a critical monoterpene precursor of high-value...
CRISPR/Cas9 mediates precise and efficient gene editing in Xanthomonas oryzae pv. oryzae
December 2026
Xanthomonas oryzae pv. oryzae (Xoo), a non-obligate phytopathogen that causes bacterial leaf blight, poses a severe threat to rice production. Although the clustered regularly interspaced short palindromic...
Remodeling memory T cells with chemotherapy and immune checkpoint inhibitors as host pre-conditioning to empower in vivo CAR-T therapy
December 2026
Chemotherapy and immune checkpoint inhibitors (ICIs) are widely utilized in cancer treatment, exerting not only direct cytotoxic effects on tumor cells but also significantly reshaping the systemic...
The PreKit platform: Cryptic gene clusters activation and high-titer compound production
December 2026
The discovery of novel scaffold compounds or enzymes through genome mining of biosynthetic gene clusters (BGCs) represents cornerstone of drug development. A major obstacle, however, is that most microbial...
Transposon insertion sequencing identifies novel genes involved in product synthesis in gas-fermenting Clostridium ljungdahlii
December 2026
The efficient utilization of industrial one-carbon (C1) gases, such as carbon dioxide (CO2) and carbon monoxide (CO), has attracted widespread interest in recent years. Clostridium ljungdahlii, a key...
The RNAi machinery regulates lovastatin biosynthesis via microRNA-like RNAs in industrial Aspergillus terreus
December 2026
The RNA interference (RNAi) machinery regulates diverse cellular processes in fungi, but its functions in industrial Aspergillus terreus remain unknown. Here, we systematically investigated two industrial...
Development of an efficient genome editing system using recombinase-mediated cassette exchange in E. coli Nissle 1917 for large gene cluster integration and heterologous astaxanthin production
December 2026
Escherichia coli Nissle 1917 (EcN) is a promising chassis strain in synthetic biology, but its application is limited by inefficient genetic manipulation. This study established an efficient genetic...
Biological-chemical method for synthesizing p-menthane-3,8-diol
December 2026
The cyclic monoterpene p-menthane-3,8-diol exhibits highly effective mosquito-repellent activity. Current industrial production of PMD depends on chemically synthesized citronellal as a precursor. To...
Hfq-dependent artificial regulatory small RNAs for targeted gene expression control in the nitrogen-fixing Pseudomonas stutzeri A1501
December 2026
Artificial trans-encoded small RNAs (atsRNAs), which repress translation by base pairing with target mRNAs, have proven to be powerful tools for regulation of gene expression for reconstructing cell...
Heterologous expression of hydroxylase coupled with pathway optimization enables highly efficient ʟ-DOPA production in Corynebacterium glutamicum
December 2026
ʟ-3,4-dihydroxyphenylalanine (ʟ-DOPA), the direct precursor of the neurotransmitter dopamine, is a cornerstone therapeutic agent for Parkinson's disease. In this study, Corynebacterium glutamicum was...
In situ continuous evolution of native transporter HGT1 unlocks xylose utilization in Kluyveromyces lactis
December 2026
Efficient xylose valorization is a prerequisite for the industrial application of Kluyveromyces lactis in lignocellulosic biorefining, yet the inefficiency of transmembrane transport remains a significant...
OmniEdit: A unified CRISPR/Cas9 platform for precise genome engineering and strain optimization of the Cordyceps militaris cell factory
December 2026
Cordyceps militaris serves as a critical microbial cell factory for high-value bioactive compounds; however, the scarcity of versatile and sophisticated genome-editing toolsets significantly restricts...
Unconditional de novo generation and functional validation of synthetic promoters with a diffusion model
December 2026
Generative neural networks enable de novo design of synthetic genetic elements. Here, we present DiPro, an unconditional diffusion model that learns E. coli promoter features to generate novel sequences...