Engineering Kluyveromyces marxianus for 3-hydroxypropionic acid production at elevated temperature from Jerusalem artichoke tubers and crude glycerol
September 2026
This study engineered the thermotolerant yeast Kluyveromyces marxianus to produce 3-hydroxypropionic acid (3-HP), a key precursor for biodegradable plastics, via the malonyl-CoA pathway using non-food...
Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates
September 2026
Succinate is a biobased platform chemical with wide applications in food, pharmaceuticals, and biodegradable polymers such as polybutylene succinate. Despite advances in microbial fermentation, cost-effective...
Exploration of endogenous signal peptides and tandem linkage strategies for enhanced heterologous protein secretion in Lacticaseibacillus paracasei
September 2026
Lacticaseibacillus paracasei (L. paracasei), a probiotic bacterium commonly found in the intestinal tract and fermented products, has been utilized as a tool for generating various bioactive components....
Advances in engineering and applications of synthetic phase-separated membraneless organelles in biotechnology
September 2026
Membraneless organelles (MLOs) formed through liquid-liquid phase separation (LLPS) constitute crucial dynamic microenvironments within cells, capable of selectively concentrating specific molecules...
Sustainable production of glutaric acid in microbial cell factories: Current advances and future prospects
September 2026
Glutaric acid is a significant C5 dicarboxylic acid, extensively utilized in the chemical industry, medicine, and biomaterials. In recent years, the advancement of synthetic biology and metabolic engineering...
A novel LPS-dependent outer membrane-anchoring mechanism for T9SS substrates enables engineered enzyme display and whole-cell PET degradation in Cytophaga hutchinsonii
September 2026
Robust surface display systems are crucial for engineering Gram-negative bacteria as whole-cell biocatalysts. In the efficient cellulose degrader Cytophaga hutchinsonii, cellulases are secreted by the...
Targeted identification of new phaterpenes and elucidation of the relevant biosynthetic pathway in Streptomyces phaeochromogenes OSK-123
September 2026
Sesquiterpenes have highly diverse chemical structures and biological activities, resulting in considerable interest in terms of their application and exploration of new analogs. In this study, we identified...
Metabolic engineering and adaptive laboratory evolution enhance squalene production in Yarrowia lipolytica
September 2026
Squalene, a multifunctional natural compound with diverse bioactivities, has significant potential in the nutraceutical and health industries. Microbial synthesis using engineered cell factories represents...
Metabolic engineering of endogenous MEP pathway for enhanced lycopene production in Escherichia coli
September 2026
Microbial cell factories represent the primary approach for heterologous lycopene synthesis, where gene source selection and pathway regulation have been demonstrated to have a significant impact on...
High-level prodigiosin production in Pseudomonas putida enabled by combinatorial metabolic engineering
September 2026
Prodigiosin, a bioactive tripyrrole pigment, exhibits a broad spectrum of biological activities-including antimicrobial, anticancer, and antimalarial properties-thereby holding significant promise for...
Reconstructing the hydrogenobyrinic acid synthetic toolkit by combining cell-free systems and metabolic engineering
September 2026
Hydrogenobyrinic acid (HBA) is a pivotal intermediate in the aerobic biosynthesis of cobalamin (vitamin B12), representing a stable, metal-free corrin precursor and a useful substrate for both biological...
A novel TetR-type repressor directly modulates precursor supply and utilization for erythromycin biosynthesis
September 2026
Antibiotic biosynthesis in actinomycetes is controlled by various transcription factors, with TetR family regulators (TFRs) serving as important modulators of this process. We previously discovered...
De novo biosynthesis of eriocitrin in Saccharomyces cerevisiae through deep learning-guided enzyme screening and systematic metabolic engineering
September 2026
Eriocitrin, a flavanone-7-O-disaccharide known for its antioxidant and anti-inflammatory properties, holds considerable promise for use in functional foods and pharmaceuticals. However, its large-scale...
Precise l-threonine-to-l-isoleucine pathway regulation for engineering high-efficiency whole-cell biocatalysts
June 2026
l-Isoleucine (L-Ile), a critical branched-chain amino acid with diverse applications in food, pharmaceutical, and cosmetic industries, is difficult to produce efficiently at scale in microbial systems...
Truncation of LPD1 promoter and adaptive evolution increase cytosolic acetyl-CoA supply in yeast
June 2026
Acetyl-CoA is a central metabolic intermediate that serves as a key precursor for the biosynthesis of high-value compounds such as terpenoids. However, its compartmentalization within Saccharomyces...
Combinatorial engineering of enzyme and pathway for efficient β-farnesene bioproduction in Yarrowia lipolytica
June 2026
β-farnesene, a natural sesquiterpene compound, has gained significant attention due to its versatile applications in agriculture, industry, biofuels, and related fields. Microbial biosynthesis offers...
Multi-omics elucidation of resource allocation for enhanced ethanol production via precise glucose control in anaerobic Saccharomyces cerevisiae fermentation
June 2026
Ethanol, a high-demand clean energy source, is primarily produced via fed-batch fermentation in industrial settings. Although our previous study identified an optimal glucose concentration of 30 g/L...
High-level production of vitamin K2 in Escherichia coli via modular molecular engineering
June 2026
Menaquinone-7 (MK-7), a key form of vitamin K2 with wide-ranging nutritional and pharmaceutical applications, has attracted increasing interest for microbial production. Here, we developed an integrated...
Engineered dual-fluorescence functional nucleic acid-based CRISPR/Cas12a biosensor for label-free ratiometric detection of site-specific DNA methylation
June 2026
The CRISPR/Cas12a system holds significant promise for biomedical applications. Nevertheless, the commonly used reporter, fluorophore–quencher-labeled substrates, is hindered by labor-intensive synthesis...
Defect-complementation homologous recombination: A novel strategy for precise genome engineering of virulent phages
June 2026
Engineered bacteriophages (phages) have been developed to overcome the limitations of natural phage therapies and serve as precision-targeted agents against drug-resistant bacterial infections. However,...
Combining multiple metabolic strategies for efficient production of longifolene in Saccharomyces cerevisiae
June 2026
Longifolene is a sesquiterpene commonly found in the heavy turpentine oil of pine plants, with various applications ranging from pest control and fragrance production to synthetic biofuels. While S....
Engineering ω-transaminase for efficient dihydroxyacetone transamination in serinol biosynthesis starting from methanol
June 2026
Serinol (2-amino-1,3-propanediol) is an important pharmaceutical intermediate, but conventional chemical or microbial routes are hampered by high energy demand, product toxicity, or complex regulation....
Synthesis of tunable copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate by engineered Halomonas bluephagenesis and their characterizations
June 2026
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with a 0–30 mol% controllable range of 3HV ratios was produced by Halomonas bluephagenesis (H. bluephagenesis) and characterized. An endogenous plasmid...
Programmable large-cargo integration: Overcoming size constraints for next-generation gene therapy
June 2026
The emergence of base and prime editors—genome editing tools that avoid double-strand breaks (DSBs)—has enabled precise point mutations, insertions, inversions, deletions, and substitutions, which accelerates...
Functions and optimization of soft law in the international governance of synthetic biology: The predicament of hard law vs. the rise of soft law
June 2026
Synthetic biology, as an emerging field that integrates life sciences and engineering technology, is driving profound transformations in global science, ethics, and legal systems. In international legal...