Discovering peptides and computational investigations of a multiepitope vaccine target Mycobacterium tuberculosis
September 2024
Mycobacterium tuberculosis (MTB) is the causative agent of tuberculosis (TB), a prevalent airborne infectious disease. Despite the availability of the Bacille Calmette-Guerin vaccine, its global efficacy...
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Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium
September 2024
Rare actinomycete genera are highly recognized as a promising source of structurally diverse and bioactive natural products. Among these genera, Allokutzneria and Kibdelosporangium are two phylogenetically...
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Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α
September 2024
Chinese hamster ovary (CHO) cells are crucial in biopharmaceutical production due to their scalability and capacity for human-like post-translational modifications. However, toxic proteins and membrane...
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Design and engineering of logic genetic-enzymatic gates based on the activity of the human CYP2C9 enzyme in permeabilized Saccharomyces cerevisiae cells
September 2024
Gene circuits allow cells to carry out complex functions such as the precise regulation of biological metabolic processes. In this study, we combined, in the yeast S. cerevisiae, genetic regulatory...
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Multivariate modular metabolic engineering and medium optimization for vitamin B12 production by Escherichia coli
September 2024
Vitamin B12 is a complex compound synthesized by microorganisms. The industrial production of vitamin B12 relies on specific microbial fermentation processes. E. coli has been utilized as a host for...
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Analyzing and engineering of the biosynthetic pathway of mollemycin A for enhancing its production
September 2024
Mollemycin A (MOMA) is a unique glyco-hexadepsipeptide-polyketide that was isolated from a Streptomyces sp. derived from the Australian marine environment. MOMA exhibits remarkable inhibitory activity...
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Environment and synthetic biology
September 2024
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Dynamics design of a non-natural transcription factor responding to androst-4-ene-3,17-dione
September 2024
The production of androst-4-ene-3,17-dione (AD) by the steroidal microbial cell factory requires transcription factors (TFs) to participate in metabolic regulation. However, microbial cell factory lacks...
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Combined strategies for improving the heterologous expression of a novel xylanase from Fusarium oxysporum Fo47 in Pichia pastoris
September 2024
Xylanase, an enzyme capable of hydrolyzing non-starch polysaccharides found in grain structures like wheat, has been found to improve the organizational structure of dough and thus increase its volume....
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Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections
September 2024
Polygalacturonase inhibiting proteins (PGIPs) are plant proteins involved in the inhibition of polygalacturonases (PGs), cell-wall degrading enzymes often secreted by phytopathogenic fungi. Previously,...
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ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models
September 2024
Genome-scale metabolic models (GEMs) have been widely employed to predict microorganism behaviors. However, GEMs only consider stoichiometric constraints, leading to a linear increase in simulated growth...
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Determination of the chromosomal position effects for plug-and-play application in the Myxococcus xanthus chassis cells
September 2024
The chromosomal position effect can significantly affect the transgene expression, which may provide an efficient strategy for the inauguration of alien genes in new hosts, but has been less explored...
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Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module
September 2024
In industrial fermentation processes, microorganisms often encounter acid stress, which significantly impact their productivity. This study focused on the acid-resistant module composed of small RNA...
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Design-build-test of recombinant Bacillus subtilis chassis cell by lifespan engineering for robust bioprocesses
September 2024
Microbial cell factories utilize renewable raw materials for industrial chemical production, providing a promising path for sustainable development. Bacillus subtilis is widely used in industry for...
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Biotransformation of ethylene glycol by engineered Escherichia coli
September 2024
There has been extensive research on the biological recycling of PET waste to address the issue of plastic waste pollution, with ethylene glycol (EG) being one of the main components recovered from...
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A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis
September 2024
Genetically encoded circuits have been successfully utilized to assess and characterize target variants with desirable traits from large mutant libraries. Adenosylcobalamin is an essential coenzyme...
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Filamentous morphology engineering of bacteria by iron metabolism modulation through MagR expression
September 2024
The morphology is the consequence of evolution and adaptation. Escherichia coli is rod-shaped bacillus with regular dimension of about 1.5 μm long and 0.5 μm wide. Many shape-related genes have been...
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Site-directed mutagenesis of bifunctional riboflavin kinase/FMN adenylyltransferase via CRISPR/Cas9 to enhance riboflavin production
September 2024
Vitamin B2 is an essential water-soluble vitamin. For most prokaryotes, a bifunctional enzyme called FAD synthase catalyzes the successive conversion of riboflavin to FMN and FAD. In this study, the...
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Rational engineering of homospermidine synthase for enhanced catalytic efficiency toward spermidine synthesis
September 2024
Spermidine is a naturally occurring polyamine widely utilized in the prevention and treatment of various diseases. Current spermidine biosynthetic methods have problems such as low efficiency and complex...
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Efficient conversion of aromatic and phenylpropanoid alcohols to acids by the cascade biocatalysis of alcohol and aldehyde dehydrogenases
June 2024
Benzyl and phenylpropanoid acids are widely used in organic synthesis of fine chemicals, such as pharmaceuticals and condiments. However, biocatalysis of these acids has received less attention than...
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DIProT: A deep learning based interactive toolkit for efficient and effective Protein design
June 2024
The protein inverse folding problem, designing amino acid sequences that fold into desired protein structures, is a critical challenge in biological sciences. Despite numerous data-driven and knowledge-driven...
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A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds
June 2024
The goal of this study was to use statistical optimization to change the nutritional and environmental conditions so that Streptomyces baarensis MH-133 could make more active metabolites. Twelve trials...
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Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology
June 2024
Bacteria exhibit a rich repertoire of RNA molecules that intricately regulate gene expression at multiple hierarchical levels, including small RNAs (sRNAs), riboswitches, and antisense RNAs. Notably,...
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A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger
June 2024
Aspergillus niger is a highly versatile fungal strain utilized in industrial production. The expression levels of recombinant genes in A. niger can be enhanced by increasing the copy number. Nevertheless,...
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Promoter engineering enables precise metabolic regulation towards efficient β-elemene production in Ogataea polymorpha
June 2024
Precisely controlling gene expression is beneficial for optimizing biosynthetic pathways for improving the production. However, promoters in nonconventional yeasts such as Ogataea polymorpha are always...
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