Combinatorial metabolic engineering of Mycobacterium neoaurum LY-2 for efficient androstenedione production from phytosterols
April 2027
Androstenedione (AD) is an important precursor for the synthesis of corticosteroids, sex hormones, and other high-value steroid pharmaceuticals. Microbial conversion of phytosterols provides a sustainable...
Genome-integrated engineering of Escherichia coli Nissle 1917 enables sustained gut-local biosynthesis of 5-hydroxytryptophan
April 2027
5-Hydroxytryptophan (5-HTP) is a functional precursor of serotonin; however, achieving sustained 5-HTP production in vivo by engineered microbes remains challenging. To address this, we developed a...
Strategies and applications for alleviating carbon catabolite repression
April 2027
Carbon catabolite repression (CCR) is a widespread regulatory strategy across diverse microorganisms that prioritises the utilisation of preferred carbon sources. In industrial bioprocesses, however,...
Strategy for enhanced production of A40926B0 in Nonomuraea gerenzanensis using an efficient CRISPR/AsCas12f1 system
April 2027
The global emergence of vancomycin-resistant Gram-positive pathogens underscores the urgent need for efficient production of novel lipoglycopeptide antibiotics. Dalbavancin, a last-resort therapeutic...
Development of a new recombineering system for Edwardsiella species
April 2027
Edwardsiella species are important aquaculture pathogens that also cause opportunistic infections in humans, necessitating efficient genome editing tools to study their pathogenesis and develop control...
Characterization of two key cytochrome P450 enzymes in Isodon amethystoides and de novo production of 3α,15β-dihydroxy-ent-kaurene in yeast
April 2027
Glaucocalyxin A (GLA), a well-known ent-kaurene diterpenoid, has emerged as a high-value bioactive natural product in pharmaceutical research owing to its potent and broad-spectrum activities. To date,...
Ribosome engineering enhances genetic code expansion in Saccharomyces cerevisiae
April 2027
Genetic code expansion enables the site-specific installation of noncanonical amino acids (ncAAs) into proteins, but its limited efficiency in eukaryotes remains a major barrier to broader application....
Directed biosynthesis of the designer peptidyl nucleoside antibiotics featuring a simplified azetidine-containing moiety
April 2027
Fungal and fungal-like pathogen infections pose escalating threats to the global one-health, thus underscoring the urgent need to reverse this trend by accelerating the development of novel antifungal...
Biosynthesis of vanillin glucoside in Escherichia coli
April 2027
Vanillin is a widely used flavor compound in the food, cosmetic, and pharmaceutical industries. Although microbial biosynthesis offers a green alternative to conventional chemical synthesis, challenges...
Genetic engineering of cyanophages for harmful cyanobacterial bloom treatment—A mini review
April 2027
Harmful cyanobacterial blooms (HCBs) are a prominent worldwide environmental concern. Proliferation of HCBs outpaces the development of mitigation and management technologies. Recently, cyanophages...
Artificial gene clusters designed for simultaneous multi-gene expression enable reconstruction of metabolite biosynthesis pathways in Trichoderma reesei
April 2027
The filamentous fungus Trichoderma reesei represents a highly promising chassis cell for the efficient synthesis of both protein and non-protein metabolites. Despite the availability of diverse genetic...
Hijacking host RNA polymerase for processive in situ mutagenesis
April 2027
Targeted mutagenesis is a crucial tool for in vivo continuous evolution. However, existing methods for long-range targeted mutagenesis in vivo often face limitations such as low efficiency, poor host...
A 6His-2Flag dual-tag strategy enables high-yield production of antimicrobial peptide N6 in Pichia pastoris to combat multidrug-resistant Klebsiella pneumonia
April 2027
In response to the global threat posed by multidrug-resistant Klebsiella pneumoniae, this study established an efficient heterologous expression system for the antimicrobial peptide N6 in Pichia pastoris...
Harnessing synthetic microbial communities for desert vegetation restoration
April 2027
The core bottleneck for desert vegetation restoration is extreme water stress during early seedling establishment, with hydraulic failure as the primary lethal mechanism. Traditional engineering measures...
Reassigning an amber codon to selenocysteine using a functional cysteine suppression pair in Escherichia coli
March 2027
Selenoproteins, characterized by the presence of selenocysteine (Sec) residues, are widely distributed across all domains of life. The unique attributes of selenoproteins are conferred by Sec, which...
Transfer of chloroplast rbcL and atpB genes to nucleus enables partial rescue of photoautotrophic growth of Chlamydomonas
March 2027
An ancient cyanobacterium has evolved through endosymbiosis to form extant chloroplasts of eukaryotic algae and higher plants. During this process, most genes have been transferred to the nuclear genomes....
System reconstruction of Bacillus licheniformis for efficient expression of alkaline protease
March 2027
Industrial enzymes are widely used in diverse applications, but low productivity limits their further widespread utilization. This research aimed to develop high-performance alkaline protease (AprE)...
Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
March 2027
Dalbavancin is a potent next-generation lipoglycopeptide antibiotic, but the biosynthesis of its essential precursor, A40926B0, is severely hindered in its native producer Nonomuraea gerenzanensis....
An orthogonal transcription system enables high-efficiency rescue of measles virus-based vaccine vectors against multiple pathogens
March 2027
Recombinant measles virus (rMeV) vectors are promising platforms for vaccine development against emerging infectious diseases due to their safety, stability, and potent immunogenicity. However, conventional...
Systematic evaluation of the DNA repair machinery facilitated the development of efficient genome editing tools for the red yeast Rhodotorula toruloides
March 2027
The red yeast Rhodotorula toruloides is a robust lipid producer, and its biotechnology applications have been expanded through genetic engineering based on the non-homologous end joining (NHEJ) pathway....
EINN: An enzyme-informed neural network guided by an enzyme-constrained genome-scale metabolic model
March 2027
Predicting cellular metabolism from molecular data is a central challenge in systems biology, with direct implications for engineering microbial cell factories. Genome-scale metabolic models (GEMs)...
Toward engineered microbial chassis for growth–production balancing
March 2027
The inherent conflict between cellular growth and product synthesis, arising from limited resource allocation and metabolic burden, fundamentally constrains the performance of microbial chassis. Traditional...
Metabolic engineering of Escherichia coli for efficient production of d-phenylglycine
March 2027
d-phenylglycine (D-PHG) is a valuable building block extensively used in the synthesis of β-lactam antibiotics and other high-value pharmaceuticals. However, its conventional chemical production relies...
Alternative splicing-induced thioesterase domain deficiency drives the production of natural orsellinic acid derivatives with a pantetheine moiety in nonreducing polyketide synthases
March 2027
Nonreducing polyketide synthase Preu6 catalyzes didepside formation by interaction of starter acyl transferase and thioesterase (TE) domains. Here, we show that an aberrant transcript, preu6β, is generated...
Unlocking raw-corncob consolidated bioprocessing for malic acid production by DyP-enabled lignin deconstruction in Myceliophthora thermophila
March 2027
l-Malic acid is an important C4 platform chemical, but its direct production from raw lignocellulosic biomass by consolidated bioprocessing (CBP) remains constrained by biomass recalcitrance. Here,...