Synthetic biology techniques to tackle heavy metal pollution and poisoning
September 2022
The requirement for natural resources and energy increases continually with the increase in population. An inevitable result of this is soil, water, and air pollution with diverse pollutants, including...
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Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest
September 2022
Electroactive microorganisms (EAMs) play important roles in biogeochemical redox processes and have been of great interest in the fields of energy recovery, waste treatment, and environmental remediation....
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Enhanced cobalamin biosynthesis in Ensifer adhaerens by regulation of key genes with gradient promoters
September 2022
Cobalamin is an essential human vitamin widely used in the pharmaceutical, food, and feed additive industries and currently produced by bacteria or archaea. Ensifer adhaerens HY-1 is an industrial strain...
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Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets
September 2022
In silico genome mining provides easy access to secondary metabolite biosynthetic gene clusters (BGCs) encoding the biosynthesis of many bioactive compounds, which are the basis for many important drugs...
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Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation
September 2022
Cytochrome P450 enzymes (CYPs) catalyze a series of C–H and C=C oxygenation reactions, including hydroxylation, epoxidation, and ketonization. They are attractive biocatalysts because of their ability...
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Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins
September 2022
Piericidins are a large family of bacterial α-pyridone antibiotics with antitumor activities such as their anti-renal carcinoma activity exhibited recently in nude mice. The backbones of piericidins...
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CO2-elevated cell-free protein synthesis
September 2022
Gases are the vital nutrition of all organisms as the precursor of metabolism pathways. As a potential biological process, protein synthesis is inevitably regulated by gas transport and utilization....
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Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region on Lithocarpus polystachyus Rehd flavonoids
September 2022
Metabolite biosynthesis is regulated by gene expression, which is altered by DNA methylation in the promoter region. Chalcone isomerase (CHI) gene encodes a key enzyme in the Lithocarpus polystachyus...
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Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling
September 2022
Metabolomics is an essential discipline in omics technology that promotes research on the biology of microbial systems. Streptomyces albus J1074 is a model organism used in fundamental research and...
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A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli
September 2022
Itaconic acid (ITA), an effective alternative fossil fuel, derives from the bypass pathway of the tricarboxylic acid (TCA) cycle. Therefore, the imbalance of metabolic flux between TCA cycle and ITA...
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Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies
September 2022
Bacterial communities form biofilms on various surfaces by synthesizing a cohesive and protective extracellular matrix, and these biofilms protect microorganisms against harsh environmental conditions....
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Metabolic engineering of Yarrowia lipolytica for scutellarin production
September 2022
Scutellarin related drugs have superior therapeutic effects on cerebrovascular and cardiovascular diseases. Here, an optimal biosynthetic pathway for scutellarin was constructed in Yarrowia lipolytica...
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Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces
September 2022
The application of the valuable natural product thaxtomin A, a potent bioherbicide from the potato scab pathogenic Streptomyces strains, has been greatly hindered by the low yields from its native producers....
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Multiplexed site-specific genome engineering in Mycolicibacterium neoaurum by Att/Int system
September 2022
Genomic integration of genes and pathway-sized DNA cassettes is often an indispensable way to construct robust and productive microbial cell factories. For some uncommon microbial hosts, such as Mycolicibacterium...
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Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens
September 2022
Bacillomycin D is a cyclic lipopeptide produced by Bacillus amyloliquefaciens fmbJ. At present, no relevant report has described the combinatorial biosynthesis of bacillomycin D. Due to the strong biosynthetic...
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Efficient fatty acid synthesis from methanol in methylotrophic yeast
Available online 30 September 2022
Methanol is an attractive C1 feedstock with high abundance and low cost in bio-manufacturing. However, the metabolic construction of cell factories to utilize methanol for chemicals production remains...
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Construction of microbial chassis for terpenoid discovery
Available online 29 September 2022
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Editoral for “Biointelligent Manufacturing in Memorium of Arnold (Arny) L. Demain”
Available online 28 September 2022
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Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains
June 2022
Non-ribosomal peptide synthetases (NRPSs) are attractive targets for biosynthetic pathway engineering due to their modular architecture and the therapeutic relevance of their products. With catalysis...
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Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture
June 2022
Resveratrol, a valuable plant-derived polyphenolic compound with various bioactivities, has been widely used in nutraceutical industries. Microbial production of resveratrol suffers from metabolic burden...
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Carbon-nitrogen bond formation to construct novel polyketide-indole hybrids from the indole-3-carbinol exposed culture of Daldinia eschscholzii
June 2022
A plenty of cytochrome P450s have been annotated in the Daldinia eschosholzii genome. Inspired by the fact that some P450s have been reported to catalyze the carbon-nitrogen (C–N) bond formation, we...
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Regulation and management of the biosecurity for synthetic biology
June 2022
Synthetic biology (SynBio) is a high-profile interdiscipline combining engineering with science. As a dual-purpose discipline, SynBio is bringing large changes to many fields and providing great benefits...
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Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima
June 2022
Nucleic acid sensing is a 3 decades old but still challenging area of application for different biological sub-domains, from pathogen detection to single cell transcriptomics analysis. The many applications...
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