Enhanced l-serine synthesis in Corynebacterium glutamicum by exporter engineering and Bayesian optimization of the medium composition
September 2025
l-serine is a versatile, high value-added amino acid, widely used in food, medicine and cosmetics. However, the low titer of l-serine has limited its industrial production. In this study, a cell factory...
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Nonglycosidic C–O bond formation catalyzed by a bifunctional pseudoglycosyltransferase ValL
September 2025
The C7N antibiotic validamycin A is an antifungal agent widely used as a crop protectant. It comprises a validoxylamine A unit linked to a glucose moiety, which is formed through a nonglycosidic C–N...
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Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production
September 2025
Xylose is a major component of lignocellulose, and it is crucial to enable microorganisms the capability of efficient xylose utilization for economical lignocellulosic biochemical production. Zymomonas...
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T7 RNA polymerase-guided base editor for accelerated continuous evolution in Bacillus subtilis
September 2025
Targeted in vivo hypermutation mediated by base deaminase-T7 RNA polymerase (T7 RNAP) fusions promotes genetic diversification and accelerates continuous directed evolution. Due to the lack of a T7RNAP...
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dTSR enables efficient improvement of heterologous production of spinosad in Saccharopolysporaerythraea
September 2025
Spinosad (spinosyns A and D) is a highly effective and environmentally friendly insecticide widely used for pest control. However, the difficulty of genetic manipulation in the original strain, Saccharopolyspora...
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Ty retrotransposon element based multiple integration toolkit for Saccharomyces cerevisiae
September 2025
Extra-high-level overexpression of single or multiple specific proteins by integrating specific genes in the genome is vital to achieve the stable and efficient production of target proteins and metabolites...
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Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution
September 2025
l-carnitine is a quaternary amine compound essential for eukaryotic metabolism. It is mainly involved in the oxidative decomposition of medium-and long-chain fatty acids and provides energy for the...
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Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases
September 2025
Recent advancements in data technology offer immense opportunities for the discovery and development of new enzymes for the green synthesis of chemicals. Current protein databases predominantly prioritize...
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Promoter engineering for enhanced 3-hydroxypropionic acid production in Pichia pastoris
September 2025
Enabling tools are essential for facilitating the methanol bioconversion in Pichia pastoris. However, there is still a relative lack of promoters that can stably express high levels without being affected...
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DNA-CTMF: Reconstruct high quality image from lossy DNA storage via Pixel-Base codebook and median filter
September 2025
Limited by uncertain base errors in DNA storage, additional correction measures may introduce redundancy or even expand errors, resulting in poor reconstructed image. DNA-CTMF is proposed to reconstruct...
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CgAS, a gene encoding anthranilic acid synthase, contributes to tryptophan biosynthesis and enhanced chaetoglobosin A production in Chaetomium globosum W7
September 2025
Chaetoglobosin A (cheA) is a complex indole alkaloid exhibiting preferential cytotoxicity against plant pathogens, parasites, and tumor cells. However, the limited production and high synthesis costs...
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Engineering the cytochrome P450 to enhance parthenolide production in Saccharomyces cerevisiae
September 2025
Parthenolide is confirmed to be an important component of the anticancer drug—ACT001. However, parthenolide biosynthesis in Saccharomyces cerevisiae (S. cerevisiae) was greatly hindered by the low conversion...
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High-yield production and characterization of functional Kir2.1 ion channel using E. coli cell-free protein synthesis system
September 2025
A family of inwardly-rectifying potassium (Kir) channels plays a key role in the regulation of cellular potassium (K+) balance, affecting muscle, nerve and immune function. Kir channels are comprised...
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Network of nicotinamide mononucleotide biosynthesis: substrate tracking, rate-limiting enzyme, and regulatory strategy
September 2025
Nicotinamide mononucleotide (NMN) is a nucleotide of significant biological importance, found abundantly in various foods such as meat, fruits, and vegetables. Recently, its potential effects in delaying...
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Biosynthesis of lanosterol in Escherichia coli
September 2025
Lipid composition represents a significant differentiator across the three domains (eukaryotes, bacteria, and archaea) of cellular life. Eukaryotes possess distinct lipids, such as sterols and sphingolipids,...
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Identification, characterization, and application of an NADPH-dependent carbonyl reductase in Saccharomyces cerevisiae AS2.346 for improving production of (13R,17S)-ethyl secol
September 2025
Levonorgestrel is currently the most widely used contraceptive, with advantages that include rapid oral absorption and high bioavailability. Among the various synthetic methods employed in its production,...
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Highly efficient synthesis of the natural product β-himachalene in yeast and its potential as a novel anti-HSV-1 therapeutic agent
September 2025
Terpenoids, a high-value natural product family in nature, have garnered significant attention due to their diverse pharmaceutical activities. However, the current supply of most structurally complex...
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Robust production of N-acetyl-glucosamine in engineered Escherichia coli from glycerol-glucose mixture
September 2025
Achieving a balanced cell growth and biosynthesis of target products is a pivotal challenge confronted in the realm of green biomanufacturing of chemicals, and it holds the promise of significantly...
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Metabolic reprogramming and computation-aided protein engineering for high-level de novo biosynthesis for 2-phenylethanol in Pichia pastoris
September 2025
2-Phenylethanol (2-PE), an aromatic compound with a characteristic rose fragrance, is extensively used in the food and cosmetic industries as a flavoring and fragrance agent. Due to limitations in obtaining...
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From reactants to products: computational methods for biosynthetic pathway design
September 2025
One of the main goals in synthetic biology is to produce value-added compounds from available precursors using enzymatic approaches. The construction of biosynthetic pathways for synthesizing target...
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Construction of a synthetic biology toolkit for the genetic manipulation in the cellulose-producing strain Kosakoniaoryzendophytica
September 2025
Bacterial cellulose (BC), a robust and highly crystalline nanomaterial composed of glucose polymers, exhibits exceptional properties including superior water retention capacity, biocompatibility, and...
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Morphology-engineered alleviation of mycelial aggregation in Streptomyces chassis for potentiated production of secondary metabolites
September 2025
The genus Streptomyces exhibits a complex life cycle of morphological differentiation and an extraordinary capacity to produce numerous bioactive secondary metabolites. In submerged cultures, Streptomyces...
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Construction of a genome-engineered stable 5-aminolevulinic acid producing Corynebacterium glutamicum by increasing succinyl-CoA supply
September 2025
5-Aminolevulinic acid (5-ALA), a versatile precursor for tetrapyrrole derivatives (such as heme, chlorophyll, and cobalamin), drives advancing microbial cell factories to meet growing biomedical and...
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Metabolic engineering Saccharomyces cerevisiae for de novo biosynthesis of hydroxycinnamoyl glycerols
Available online 17 July 2025
Hydroxycinnamoyl glycerols (HCGs) are a class of bioconjugates characterized by the esterification of hydroxycinnamic acids with glycerol. Owing to their diverse biological activities, HCGs are considered...
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Decoding metabolic trade-offs in Halomonas elongata: Chemostat-based flux remodeling for industrial ectoine biosynthesis
Available online 17 July 2025
Halomonas elongata, a moderately halophilic γ-proteobacterium of industrial interest, serves as a microbial cell factory for ectoine—a high-value compatible solute extensively utilized in biopharmaceuticals...
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