Defense-associated reverse transcriptases define an emerging class of noncanonical DNA-writing systems
December 2026
Defense-associated reverse transcriptases (DRTs) are emerging as a mechanistically diverse class of bacterial antiviral enzymes. Rather than acting simply as RNA-to-DNA copying proteins, they generate...
Eukaryotic cell-free protein synthesis: Chassis diversification, system engineering, and emerging applications
December 2026
The eukaryotic cell-free protein synthesis (CFPS) system, endowed with intrinsic post-translational modification capabilities and a complex molecular chaperone network, efficiently synthesizes functional...
Development and clinical validation of a multiplex visual POCT platform based on RT-RAA-CRISPR/Cas12a for porcine diarrheal viruses
December 2026
Porcine epidemic diarrhea virus (PEDV), porcine rotavirus A (PoRVA), and porcine sapelovirus (PSV) are major causative agents of severe diarrhea in swine and frequently occur as co-infections, leading...
The acetylation mechanism and engineering strategy of isocitrate lyase for optimizing biosynthesis in Escherichia coli
December 2026
Lysine acetylation is a widespread post-translational modification that fine-tunes cell physiology and metabolism. Yet its application in metabolic engineering remains underexplored. Here, we report...
Engineering Kluyveromyces marxianus for 3-hydroxypropionic acid production at elevated temperature from Jerusalem artichoke tubers and crude glycerol
September 2026
This study engineered the thermotolerant yeast Kluyveromyces marxianus to produce 3-hydroxypropionic acid (3-HP), a key precursor for biodegradable plastics, via the malonyl-CoA pathway using non-food...
Exploration of endogenous signal peptides and tandem linkage strategies for enhanced heterologous protein secretion in Lacticaseibacillus paracasei
September 2026
Lacticaseibacillus paracasei (L. paracasei), a probiotic bacterium commonly found in the intestinal tract and fermented products, has been utilized as a tool for generating various bioactive components....
Advances in engineering and applications of synthetic phase-separated membraneless organelles in biotechnology
September 2026
Membraneless organelles (MLOs) formed through liquid-liquid phase separation (LLPS) constitute crucial dynamic microenvironments within cells, capable of selectively concentrating specific molecules...
Metabolic engineering and adaptive laboratory evolution enhance squalene production in Yarrowia lipolytica
September 2026
Squalene, a multifunctional natural compound with diverse bioactivities, has significant potential in the nutraceutical and health industries. Microbial synthesis using engineered cell factories represents...
Metabolic engineering of endogenous MEP pathway for enhanced lycopene production in Escherichia coli
September 2026
Microbial cell factories represent the primary approach for heterologous lycopene synthesis, where gene source selection and pathway regulation have been demonstrated to have a significant impact on...
Reconstructing the hydrogenobyrinic acid synthetic toolkit by combining cell-free systems and metabolic engineering
September 2026
Hydrogenobyrinic acid (HBA) is a pivotal intermediate in the aerobic biosynthesis of cobalamin (vitamin B12), representing a stable, metal-free corrin precursor and a useful substrate for both biological...
Construction of a globally modular recombinant Saccharomyces cerevisiae chassis for ginkgolide precursor levopimaradiene production
September 2026
The production of biologically active natural products through microbial metabolic factories, such as Saccharomyces cerevisiae, represents a promising approach to achieving green and sustainable production....
Multistep metabolic engineered Klebsiella oxytoca for efficient l-leucine production
September 2026
l-Leucine is widely applied in food, feed and medical industries. In this work, an efficient l-leucine producing strain Klebsiella oxytoca LKO-14 was constructed based on an l-valine producer K. oxytoca...
Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system
September 2026
Bacillus subtilis is widely employed for lignocellulose degradation. However, wild-type strains typically exhibit low and incomplete cellulase activities. This study aimed to engineer recombinant B....
De novo biosynthesis of 1,5-diamino-2-hydroxypentane from glucose via combination of fermentation and whole-cell catalysis
September 2026
1,5-Diamino-2-hydroxypentane (2-OH-PDA) is a novel aliphatic β-amino alcohol with potential for a wide range of applications, and its efficient and green synthesis has attracted increasing attention....
Utilization, metabolic regulation and applications of hydrophobic substrates in Yarrowia lipolytica
September 2026
The efficient use of hydrophobic substrates such as n-alkanes and fatty acids as carbon and energy sources is an important native ability of Yarrowia lipolytica. This review summarizes the crucial characteristics...
High-yield production of hydroxytyrosol through metabolic engineering of Yarrowia lipolytica
September 2026
Hydroxytyrosol (HT) is a natural antioxidant widely employed in the food production, pharmaceutical, and cosmetic industries. Microbial fermentation for HT production holds significant potential for...
De novo biosynthesis of putrescine from glucose using engineered Escherichia coli in a two-step process
September 2026
Putrescine, is a key monomer for the synthesis of high-performance polymer materials. However, its sustainable microbial production remains challenging in yield and efficiency. In this study, we developed...
XRE-type transcriptional regulator ProR controls prodigiosin synthesis in Serratia marcescens JNB5-1
September 2026
The gram-negative bacterium Serratia marcescens produces various secondary metabolites, including prodigiosin, althiomycin, and serratamolide. Among these, prodigiosin exhibits multiple biological activities...
Engineered Aspergillus niger enables high-yield heterologous kojic acid production
September 2026
Industrial-scale production of kojic acid (KA)—valued in cosmetics, food, and pharma—has been hindered by limited metabolic engineering strategies for native producers (e.g., Aspergillus oryzae), which...
Development of mycelium-reinforced dialdehyde cellulose–polyvinyl alcohol composites for emerging high-performance sustainable materials
September 2026
Dialdehyde cellulose (DAC), featuring reactive carbonyl groups introduced via oxidation of hemp-derived cellulose fibers (confirmed by FTIR spectroscopy), was synthesized to promote strong hydrogen...
Construction of a library of gene expression elements in industrial Streptomyces and its application
September 2026
Streptomycetes are key producers of natural products (NPs), and engineering these organisms represents an effective strategy for enhancing NPs production. However, genetic elements (e.g., promoters...
Physiological metabolic analysis and process optimization of hypoxia in promoting coenzyme Q10 biosynthesis and accumulation in Rhodobacter sphaeroides HY01
September 2026
Coenzyme Q10 fermentation by Rhodobacter sphaeroides (R. sphaeroides) is highly aerobic. However, its biosynthesis and accumulation are paradoxically induced under hypoxic conditions. While oxygen serves...
P450 engineering via structure-guided rational design achieves high C21-selectivity and bioconversion in steroid biosynthesis
September 2026
C21-hydroxylation is a crucial step in the synthesis of corticosteroids. For instance, C21-hydroxylase catalyzes the conversion of progesterone (PRO) into 11-deoxycorticosterone (DOC, the precursor...
Targeted identification of new phaterpenes and elucidation of the relevant biosynthetic pathway in Streptomyces phaeochromogenes OSK-123
September 2026
Sesquiterpenes have highly diverse chemical structures and biological activities, resulting in considerable interest in terms of their application and exploration of new analogs. In this study, we identified...
Enhanced spinosad production in Saccharopolyspora spinosa by employing mannose as an extracellular carbon reservoir and optimizing acetyl-CoA supply pathway
September 2026
Spinosad, a potent broad-spectrum insecticidal polyketide produced by Saccharopolyspora spinosa, faces limitations in industrial-scale production due to inherent inefficiencies in its native biosynthetic...