A multifunctional switch for label-free CRISPR/Cas12a sensor with self-driven amplification
December 2025
MicroRNA (miRNA) is promising candidate for non-invasive diagnostic biomarker. Conventional CRISPR/Cas12a-based miRNA detection systems are constrained by reliance on reverse transcription, nucleic...
Development of precise genome editing and multi-copy integration tools in Hansenula polymorpha DL-1
December 2025
Hansenula polymorpha DL-1 is a thermotolerant yeast capable of utilizing multiple renewable carbon sources, making it a promising microbial cell factory for sustainable manufacturing. However, advanced...
Enzyme and cofactor engineering to increase d-xylonate dehydratase activity for improved d-1,2,4-butanetriol production from d-xylose
December 2025
d-1,2,4-Butanetriol (BTO), a C4 platform compound, is widely used in fields such as military and pharmaceuticals. Biosynthesis of d-1,2,4-BTO from lignocellulose-derived d-xylose presents a promising...
Metabolic engineering Saccharomyces cerevisiae for de novo biosynthesis of hydroxycinnamoyl glycerols
December 2025
Hydroxycinnamoyl glycerols (HCGs) are a class of bioconjugates characterized by the esterification of hydroxycinnamic acids with glycerol. Owing to their diverse biological activities, HCGs are considered...
Decoding metabolic trade-offs in Halomonas elongata: Chemostat-based flux remodeling for industrial ectoine biosynthesis
December 2025
Halomonas elongata, a moderately halophilic γ-proteobacterium of industrial interest, serves as a microbial cell factory for ectoine—a high-value compatible solute extensively utilized in biopharmaceuticals...
Biosynthesis of irregular monoterpene lavandulol in Saccharomyces cerevisiae
December 2025
Lavandulol, the primary chemical constituent of lavender essential oil, is an irregular monoterpene present in Lavandula angustifolia. It has been employed in the spice and cosmetic industries owing...
Machine learning-assisted rational design and evolution of novel signal peptides in Yarrowia lipolytica
December 2025
Microbial proteins hold great promise as sustainable alternatives for future protein sources, and oleaginous yeast Yarrowia lipolytica has emerged as a recognized platform for heterologous protein expression...
Development of a highly efficient p-coumaric acid-responsive biosensor in Saccharomyces cerevisiae
December 2025
Developing biosensors to monitor and regulate intracellular biosynthesis pathways can significantly enhance natural product yields in microbial cell factories. This study created a novel biosensor in...
Advances in adaptive laboratory evolution applications for Escherichia coli
December 2025
Adaptive Laboratory Evolution (ALE), a well-established framework in microbial evolution research, is widely applied in synthetic biology. By simulating natural selection through controlled serial culturing,...
Engineering non-P450 3-hydroxylase for de novo synthesizes catechol-containing compounds in Escherichia coli
December 2025
Catechols (such as l-DOPA, caffeic acid and hydroxytyrosol, etc.) are a class of phenolic derivatives with ortho-hydroxyl groups which represents various bioactivities including antioxidative, anti-inflammatory,...
Decoupled tricarboxylic acid cycle and glycolysis of Corynebacterium glutamicum combined with acetate supply promote high-yield biosynthesis of l-homoserine
December 2025
l-Homoserine is a valuable intermediate with broad applications in the food, pharmaceutical, and chemical industries. Although Corynebacterium glutamicum has been engineered for the efficient biosynthesis...
Engineered membraneless organelles in Corynebacterium glutamicum for enhanced indigoidine biosynthesis and antimicrobial peptide production
December 2025
Liquid-liquid phase separation (LLPS)-driven membraneless organelles (MLOs) have been employed to enhance metabolic efficiency in various microbial cell factories. However, their application in the...
Construction of microbial systems for polyethylene terephthalate degradation
December 2025
Polyethylene terephthalate (PET) is one of the most widely used plastic materials, and its large-scale application has caused severe environmental pollution. Compared to traditional physical and chemical...
Engineered bacteriophage-based bioimaging Technology: Development and applications
December 2025
Engineered bacteriophages (phages) have emerged as powerful and versatile tools for bioimaging, owing to their natural specificity for bacterial targets and their amenability to functional modification....
Integrated protein and metabolic engineering enable high-yield biosynthesis of resveratrol derivatives in Yarrowia lipolytica
December 2025
Piceatannol and pterostilbene, two resveratrol derivatives with superior pharmacological properties, hold significant promise for therapeutic and nutraceutical applications. Despite significant progress...
Gene Surfing: An efficient and versatile tool for targeted enzyme mining in metagenomics
December 2025
Microbial community studies have established enzymes' pivotal catalytic roles in ecosystem metabolism, yet cultivation-dependent methods fail to exploit uncultured microbial enzyme resources. Metagenomics...
Engineering a drug-inducible pyroptosis platform enables precise tumor suppression in colorectal cancer
December 2025
Colorectal cancer remains a leading cause of cancer-related mortality, with long-term survival rates hindered by chemoresistance and an immunosuppressive tumor microenvironment. Gene-based therapies...
Development and application of synthetic biology tools for improved production of human breast milk components
December 2025
Human breast milk (HBM) is composed of various components that are crucial for providing essential nutrients and enhancing infant immune system. Recently, the synthesis of HBM components through microbial...
Engineering robust Saccharomyces cerevisiae cell factory for improving fatty alcohol biosynthesis
December 2025
Engineering yeast cell factories is a feasible approach to produce value chemicals from renewable feedstocks. However, during the production process, reprogramming of the internal metabolic pathways...
Genome mining and characterization of a heme-dependent enzyme catalyzing intermolecular Nitrogen–Nitrogen bond formation in hydrazinosuccinic acid biosynthesis
December 2025
Nitrogen–nitrogen (N–N) bond-forming enzymes are rare but play vital roles in both primary and secondary metabolism. Guided by a nitric oxide synthase (NOS)-based genome mining strategy, we report the...
De novo biosynthesis of Asperosaponin VI in Saccharomyces cerevisiae
December 2025
Asperosaponin VI (ASA VI), the primary bioactive triterpenoid saponin marker of Dipsacus asper Wall. (Chinese Pharmacopoeia 2020), possesses significant neuroprotective, anti-inflammatory, and osteogenic...
iCASRED, a scarless DNA editing tool in E. coli for high-efficiency engineering of natural product biosynthetic gene clusters
September 2025
Efficient gene cluster editing tools are one of the key techniques for discovering novel compounds encoded by silent natural product (NP) biosynthetic gene clusters (BGCs) in microbial genomes. Currently,...
T7 RNA polymerase-guided base editor for accelerated continuous evolution in Bacillus subtilis
September 2025
Targeted in vivo hypermutation mediated by base deaminase-T7 RNA polymerase (T7 RNAP) fusions promotes genetic diversification and accelerates continuous directed evolution. Due to the lack of a T7RNAP...
Engineering the cytochrome P450 to enhance parthenolide production in Saccharomyces cerevisiae
September 2025
Parthenolide is confirmed to be an important component of the anticancer drug—ACT001. However, parthenolide biosynthesis in Saccharomyces cerevisiae (S. cerevisiae) was greatly hindered by the low conversion...
Biosynthesis of lanosterol in Escherichia coli
September 2025
Lipid composition represents a significant differentiator across the three domains (eukaryotes, bacteria, and archaea) of cellular life. Eukaryotes possess distinct lipids, such as sterols and sphingolipids,...