Efficient whole-cell biotransformation for gastrodin production via glycosyltransferase engineering and process optimization
December 2026
Gastrodin is a naturally occurring phenolic glycoside derived from Gastrodia elata, which is widely used as an edible medicinal plant in functional foods and dietary supplements in East Asia. Owing...
Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
December 2026
Xiamenmycin, a prenylated benzopyran compound produced by Streptomyces xiamenensis 318, exhibits promising anti-fibrotic activity but suffers from low productivity, limiting its pharmaceutical development....
Shikimate crosstalk enables record-level homogentisic acid production in Yarrowia lipolytica
December 2026
Homogentisic acid (HGA; 2,5-dihydroxyphenylacetic acid) is an aromatic compound with diverse applications in pharmaceuticals, food, and functional materials. Industrial HGA production still relies mainly...
Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla
December 2026
Paris species are renowned for the production of important medicinal steroidal saponins (e.g., polyphyllins). UDP-dependent glycosyltransferases (UGTs) play important roles in saponin biosynthesis....
CRISPR/Cas9 mediates precise and efficient gene editing in Xanthomonas oryzae pv. oryzae
December 2026
Xanthomonas oryzae pv. oryzae (Xoo), a non-obligate phytopathogen that causes bacterial leaf blight, poses a severe threat to rice production. Although the clustered regularly interspaced short palindromic...
Remodeling memory T cells with chemotherapy and immune checkpoint inhibitors as host pre-conditioning to empower in vivo CAR-T therapy
December 2026
Chemotherapy and immune checkpoint inhibitors (ICIs) are widely utilized in cancer treatment, exerting not only direct cytotoxic effects on tumor cells but also significantly reshaping the systemic...
Transposon insertion sequencing identifies novel genes involved in product synthesis in gas-fermenting Clostridium ljungdahlii
December 2026
The efficient utilization of industrial one-carbon (C1) gases, such as carbon dioxide (CO2) and carbon monoxide (CO), has attracted widespread interest in recent years. Clostridium ljungdahlii, a key...
The RNAi machinery regulates lovastatin biosynthesis via microRNA-like RNAs in industrial Aspergillus terreus
December 2026
The RNA interference (RNAi) machinery regulates diverse cellular processes in fungi, but its functions in industrial Aspergillus terreus remain unknown. Here, we systematically investigated two industrial...
Development of an efficient genome editing system using recombinase-mediated cassette exchange in E. coli Nissle 1917 for large gene cluster integration and heterologous astaxanthin production
December 2026
Escherichia coli Nissle 1917 (EcN) is a promising chassis strain in synthetic biology, but its application is limited by inefficient genetic manipulation. This study established an efficient genetic...
Biological-chemical method for synthesizing p-menthane-3,8-diol
December 2026
The cyclic monoterpene p-menthane-3,8-diol exhibits highly effective mosquito-repellent activity. Current industrial production of PMD depends on chemically synthesized citronellal as a precursor. To...
Hfq-dependent artificial regulatory small RNAs for targeted gene expression control in the nitrogen-fixing Pseudomonas stutzeri A1501
December 2026
Artificial trans-encoded small RNAs (atsRNAs), which repress translation by base pairing with target mRNAs, have proven to be powerful tools for regulation of gene expression for reconstructing cell...
Heterologous expression of hydroxylase coupled with pathway optimization enables highly efficient ʟ-DOPA production in Corynebacterium glutamicum
December 2026
ʟ-3,4-dihydroxyphenylalanine (ʟ-DOPA), the direct precursor of the neurotransmitter dopamine, is a cornerstone therapeutic agent for Parkinson's disease. In this study, Corynebacterium glutamicum was...
Unconditional de novo generation and functional validation of synthetic promoters with a diffusion model
December 2026
Generative neural networks enable de novo design of synthetic genetic elements. Here, we present DiPro, an unconditional diffusion model that learns E. coli promoter features to generate novel sequences...
Eukaryotic cell-free protein synthesis: Chassis diversification, system engineering, and emerging applications
December 2026
The eukaryotic cell-free protein synthesis (CFPS) system, endowed with intrinsic post-translational modification capabilities and a complex molecular chaperone network, efficiently synthesizes functional...
Development and clinical validation of a multiplex visual POCT platform based on RT-RAA-CRISPR/Cas12a for porcine diarrheal viruses
December 2026
Porcine epidemic diarrhea virus (PEDV), porcine rotavirus A (PoRVA), and porcine sapelovirus (PSV) are major causative agents of severe diarrhea in swine and frequently occur as co-infections, leading...
Enhanced production of l-histidine in Escherichia coli through systematic metabolic engineering and CER controlled fermentation
December 2026
l-Histidine is an essential amino acid with important applications in pharmaceuticals and nutrition, highlighting the demand for efficient microbial production platforms. This study developed a high-performance...
Systems engineering of Escherichia coli for high-level hydroxytyrosol production
December 2026
Hydroxytyrosol (HT) is a potent polyphenolic antioxidant widely utilized in the biomedical and food industries. However, its high-level microbial biosynthesis is primarily hindered by the metabolic...
Synergistic interactions in designed bacterial consortia: Dual effects on control rusty root rot and growth promotion in ginseng
December 2026
Ginseng rusty root rot, caused by Ilyonectria robusta, is the most severe soil-borne disease affecting ginseng cultivation. A synthetic microbial community (SynCom) was developed for the first time...
A cell-free system for 1,6-hexanediamine synthesis using bulk chemical caprolactam as the feedstock
December 2026
Biomanufacturing, with its advantages of mildness, low carbon emissions, and renewability, drives the frontier of green chemistry and sustainable development. However, complex metabolic pathways and...
Engineering of fructose-6-phosphate aldolase for one-carbon conversion to mannitol in a designed biotransformation system
December 2026
Developing artificial synthetic pathways for converting one-carbon compounds into value-added chemicals represents a promising strategy for carbon-neutral manufacturing. Enzymatic C–C bond formation...
Engineered bacteria in disease diagnosis and therapy: A synthetic biology perspective
December 2026
Synthetic biology is an interdisciplinary field that integrates knowledge and techniques from modern biology and many other disciplines to design and construct novel biological systems or to modify...
Progresses of transposon research in Saccharomyces cerevisiae
December 2026
Transposons are mobile genetic elements that have attracted sustained scientific interest since their discovery in maize, evolving into indispensable tools in genetic research. Saccharomyces cerevisiae...
De novo production of (−)-limonene, (−)-carveol and (−)-carvone via engineering Rhodotorula toruloides
December 2026
De novo biosynthesis of (−)-limonene, (−)-carveol and (−)-carvone was achieved via rewiring the mevalonate pathway in the oleaginous yeast Rhodotorula toruloides. Strategies including enzyme selection,...
Two-step bioconversion of pyridoxal 5′-phosphate from pyridoxine through cofactor regeneration and pyridoxine 5′-phosphate oxidase evolution
December 2026
Pyridoxal 5′-phosphate (PLP), a vital cofactor in cellular metabolism, is a high-value compound widely used in the food and pharmaceutical industries. However, the efficient biosynthesis of PLP remains...
Engineering a heme-dependent tryptophan hydroxylase pathway in E. coli for enhanced melatonin production
December 2026
Melatonin is a high-value bioactive indoleamine broadly applied in the pharmaceutical, food and nutraceutical industries, yet its microbial production remains constrained by pathway complexity and low...