Construction of an alternative NADPH regeneration pathway improves ethanol production in Saccharomyces cerevisiae with xylose metabolic pathway
June 2024
Full conversion of glucose and xylose from lignocellulosic hydrolysates is required for obtaining a high ethanol yield. However, glucose and xylose share flux in the pentose phosphate pathway (PPP)...
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Descriptor-augmented machine learning for enzyme-chemical interaction predictions
June 2024
Descriptors play a pivotal role in enzyme design for the greener synthesis of biochemicals, as they could characterize enzymes and chemicals from the physicochemical and evolutionary perspective. This...
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The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction
June 2024
Aspergillus niger is an efficient cell factory for organic acids production, particularly l-malic acid, through genetic manipulation. However, the traditional method of collecting A. niger spores for...
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CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans
June 2024
Gluconobacter oxydans is an important Gram-negative industrial microorganism that produces vitamin C and other products due to its efficient membrane-bound dehydrogenase system. Its incomplete oxidation...
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Omics data analysis reveals the system-level constraint on cellular amino acid composition
June 2024
Proteins play a pivotal role in coordinating the functions of organisms, essentially governing their traits, as the dynamic arrangement of diverse amino acids leads to a multitude of folded configurations...
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Multi-modular metabolic engineering of heme synthesis in Corynebacterium glutamicum
June 2024
Heme, an iron-containing porphyrin derivative, holds great promise in fields like medicine, food production and chemicals. Here, we developed an engineered Corynebacterium glutamicum strain for efficient...
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Engineered microorganisms: A new direction in kidney stone prevention and treatment
June 2024
Numerous studies have shown that intestinal and urinary tract flora are closely related to the formation of kidney stones. The removal of probiotics represented by lactic acid bacteria and the colonization...
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Single carbon metabolism – A new paradigm for microbial bioprocesses?
June 2024
Increasing atmospheric carbon dioxide levels, a reduction of arable land area and the dependence of first and second generation biotechnology feedstocks on agricultural products, call for alternative,...
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Design and synthesis of synthetic promoters for consistency of gene expression across growth phases and scale in S. cerevisiae
June 2024
Metabolic engineering and synthetic biology endeavors benefit from promoters that perform consistently (or robustly) with respect to cellular growth phase (exponential and stationary) and fermentation...
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Acarbose glycosylation by AcbE for the production of acarstatins with enhanced α-amylase inhibitory activity
June 2024
Acarbose is a potent glycosidase inhibitor widely used in the clinical treatment of type 2 diabetes mellitus (T2DM). Various acarbose analogs have been identified while exploring compounds with improved...
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Antibacterial activity and mechanism of X33 antimicrobial oligopeptide against Acinetobacter baumannii
June 2024
Acinetobacter baumannii is a pathogenic bacterium widespread in human environments, especially in intensive care units, and is associated with high morbidity and infection rates. Multiple drug resistance...
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Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max
June 2024
Plants produce diverse flavonoids for defense and stress resistance, most of which have health benefits and are widely used as food additives and medicines. Methylation of the free hydroxyl groups of...
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Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production
June 2024
Due to the complicated metabolic and regulatory networks of l-serine biosynthesis and degradation, microbial cell factories for l-serine production using non-model microorganisms have not been reported....
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Engineering and finetuning expression of SerC for balanced metabolic flux in vitamin B6 production
June 2024
Vitamin B6 plays a crucial role in cellular metabolism and stress response, making it an essential component for growth in all known organisms. However, achieving efficient biosynthesis of vitamin B6...
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Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket
June 2024
Fungal bifunctional terpene synthases (BFTSs) catalyze the formation of numerous di-/sester-/tri-terpenes skeletons. However, the mechanism in controlling the cyclization pattern of terpene scaffolds...
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SemiSynBio: a new era for neuromorphic computing
Available online 18 April 2024
Neuromorphic computing has the potential to achieve the requirements of the next-generation artificial intelligence (AI) systems, due to its advantages of adaptive learning and parallel computing. Meanwhile,...
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Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch
Available online 16 April 2024
Strengthening the expression level of integrated genes on the genome is crucial for consistently expressing key enzymes in microbial cell factories for efficient bioproduction in synthetic biology....
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Determination of the chromosomal position effects for plug-and-play application in the Myxococcus xanthus chassis cells
Available online 10 April 2024
The chromosomal position effect can significantly affect the transgene expression, which may provide an efficient strategy for the inauguration of alien genes in new hosts, but has been less explored...
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Cyclization mechanism of monoterpenes catalyzed by monoterpene synthases in dipterocarpaceae
March 2024
Monoterpenoids are typically present in the secretory tissues of higher plants, and their biosynthesis is catalyzed by the action of monoterpene synthases (MTSs). However, the knowledge about these...
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Expression and antimicrobial activity of the recombinant bovine lactoferricin in Pichia pastoris
March 2024
Lactoferricin, a multifunctional peptide located in the N-terminal region of lactoferrin, has a broad-spectrum bacteriostatic activity. It is a promising candidate as a food additive and immune fortification...
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A toolbox for genetic manipulation in intestinal Clostridium symbiosum
March 2024
Gut microbes are closely related with human health, but remain much to learn. Clostridium symbiosum is a conditionally pathogenic human gut bacterium and regarded as a potential biomarker for early...
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Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway
March 2024
2′-Fucosyllactose (2′-FL) is one of the important functional oligosaccharides in breast milk. So far, few attempts on biosynthesis of 2′-FL by the salvage pathway have been reported. Herein, the salvage...
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Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917
March 2024
The probiotic bacterium Escherichia coli Nissle 1917 (EcN) holds significant promise for use in clinical and biological industries. However, the reliance on antibiotics to maintain plasmid-borne genes...
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Development of Zn2+-controlled expression system for lactic acid bacteria and its application in engineered probiotics
March 2024
Lactococcus lactis and Streptococcus thermophilus are considered as ideal chassis of engineered probiotics, while food-grade genetic tools are limited in those strains. Here, a Zn2+-controlled gene...
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March 2024
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