Enhanced microbial production of pyridoxine (Vitamin B6) in Bacillus subtilis via pathway and process optimization
March 2026
Vitamin B6 refers to a family of water-soluble B vitamin, which is essential for various physiological functions, including amino acid metabolism, neurotransmitter synthesis, and hemoglobin synthesis....
A smart gene circuit for precise regulation of tumor cell behavior
March 2026
Personalized gene circuit is a robust mode of cellular regulation that can manipulate intracellular gene expression to achieve desired functional regulation. However, the construction of logic circuits...
Structural and functional insights into the iminium ion intermediate in AsKslB-mediated Pictet-Spengler reaction
March 2026
Pictet-Spengler (PS) reactions are pivotal in the biosynthesis of bioactive alkaloids and pharmaceuticals, yet key structural details underlying their enzymatic catalysis remain insufficiently understood....
Integrated transcriptomic and metabolomic analyses uncover novel genetic targets for enhanced l-tryptophan production in Corynebacterium glutamicum
March 2026
Corynebacterium glutamicum is a promising microbial chassis for the industrial production of l-tryptophan, an essential amino acid with diverse applications and high market value. In our previous work,...
An efficient CRISPR-Cas12a tool for iterative genome editing, streamlined minimization, and payload engineering of Pseudomonas phage S1
March 2026
The ability to precisely engineer phage genomes is critical for advancing phage-based biotechnology and therapeutic development. Here, we present a high-efficiency, scarless CRISPR-Cas12a genome editing...
Engineering mixed sugar metabolic channels in Pseudomonas putida to produce vanillic acid
March 2026
Hydrolysis of lignocellulosic biomass produces the mixed sugars of glucose (60–70 %), xylose (20–30 %), and arabinose (2–20 %), etc. Using mixed sugars instead of pure glucose for microbial biosynthesis...
Putative membrane protein Cgl2168 and its variant enhance thermotolerance in Corynebacterium glutamicum
March 2026
Corynebacterium glutamicum ATCC 13032 is widely used as the chassis strain for industrial production of various amino acids, but its thermotolerance limits productivity under high temperatures. Comparative...
CRISPR-based synthetic biology toolkit development in Candida viswanthii and functional analysis of the stress responsive Ena1-like protein
March 2026
Industrial strains from the Candida genus have been applied in production of enzymes, biochemicals, and single-cell protein. However, the synthetic biology manipulation tools for Candida species remain...
Harnessing synthetic biology for tetraterpenoid astaxanthin production: Recent advances and challenges
March 2026
Astaxanthin, a potent lipid-soluble ketocarotenoid, exists in various stereoisomeric, geometric isomeric, and esterified forms. Its unique molecular configuration and biological activity confer significant...
CRISPy-web 3.0: A unified platform for multi-modal guide RNA design for CRISPR and TnpB genome editing applications
March 2026
CRISPy-web is an interactive web-based platform designed for rational guide RNA design in CRISPR-Cas9-based genome editing systems. Here, we present CRISPy-web 3.0 (https://crispy.secondarymetabolites.org/),...
Developing the protein quality control system in Streptomyces for selective translation of ultra-long full-length polyketide synthase mRNAs to enhance biosynthesis efficiency
March 2026
Genes that encode type I polyketide synthases (PKSs), which synthesize an array of invaluable drugs, typically exceed 10 kilobases in length. Truncated messenger RNAs (mRNAs) have been demonstrated...
Engineering heterologous core promoters to optimize the SES expression system for Pichia pastoris
March 2026
Synthetic biology has revolutionized microbial production by broadening the range of substrates, products, and host organisms. Pichia pastoris, a pivotal system for industrial enzyme production and...
Harnessing prokaryotic amino acid transporters for metabolic engineering: mechanisms and biotechnological applications
March 2026
As fundamental bioactive molecules and building blocks of cellular structures, amino acids play a crucial role in sustaining life, reflected in a global market valued at tens of billions of dollars....
Competitive binding of TeXlnR and TeAmyR regulates cellulase production in Talaromyces endophyticus
March 2026
Efficient cellulase production is essential for its application in green bioprocessing, yet the transcriptional regulation of cellulase biosynthesis in Talaromyces sp. remains largely uncharacterized....
Engineering genetic elements for microbial protein expression systems: Advances, challenges, applications, and prospects
March 2026
The rising global demand for sustainable protein sources poses critical challenges across food, pharmaceutical, and industrial biotechnology sectors. Microbial expression systems provide scalable and...
Amplicon-based prediction of secondary metabolic potential of microbiomes facilitates natural product discovery
March 2026
The rapid exploration of natural products not only expands chemical diversity but also plays a vital role in drug discovery by identifying bioactive agents (e.g., antibiotics), revealing novel biological...
Multiple Buster transposons from hAT superfamily displaying transposition activities in mammal cells
March 2026
TcBuster, a member of the Buster family within the hAT superfamily, has been engineered into an effective gene transfer tool for gene therapy applications. To diversify the toolkit available for efficient...
A simplified synthetic microbial community enhances resistance of crucian carp (Carassius auratus) to Aeromonas hydrophila infection through host immune activation
March 2026
Bacterial diseases represent a major bottleneck in the sustainable development of aquaculture. The gut microbiota plays a vital role in host growth and health, including the enhancement of disease resistance....
Recombinant production of amaranthin and other betalain variants with yeast cell factories
December 2025
Betalains are a class of natural pigments found in plants of the Caryophyllales order. Betanin is the dominant betalain on the food colour market, even though over 80 other variants are known. Recombinant...
Engineering T7 RNA polymerase-cascaded systems controlled by nisin and theophylline for protein overexpression and targeted gene mutagenesis in Lactococcus lactis
December 2025
Lactococcus lactis serves as an important platform for heterologous protein production, with the nisin-controlled gene expression (NICE) system being widely employed for regulated protein overexpression....
Genomic and transcriptomic insights into the molecular responses of a biocrust-derived oleaginous microalga Vischeria sp. WL1 to nitrogen depletion and recovery
December 2025
Microalgae surviving in extreme conditions evolve tolerance mechanisms to diverse environmental stresses. Environmental nitrogen limitation or nitrogen starvation poses a significant threat to the growth...
Machine learning-assisted rational design and evolution of novel signal peptides in Yarrowia lipolytica
December 2025
Microbial proteins hold great promise as sustainable alternatives for future protein sources, and oleaginous yeast Yarrowia lipolytica has emerged as a recognized platform for heterologous protein expression...
Development of a highly efficient p-coumaric acid-responsive biosensor in Saccharomyces cerevisiae
December 2025
Developing biosensors to monitor and regulate intracellular biosynthesis pathways can significantly enhance natural product yields in microbial cell factories. This study created a novel biosensor in...
Decoupled tricarboxylic acid cycle and glycolysis of Corynebacterium glutamicum combined with acetate supply promote high-yield biosynthesis of l-homoserine
December 2025
l-Homoserine is a valuable intermediate with broad applications in the food, pharmaceutical, and chemical industries. Although Corynebacterium glutamicum has been engineered for the efficient biosynthesis...