Construction of a library of gene expression elements in industrial Streptomyces and its application
September 2026
Streptomycetes are key producers of natural products (NPs), and engineering these organisms represents an effective strategy for enhancing NPs production. However, genetic elements (e.g., promoters...
Utilization, metabolic regulation and applications of hydrophobic substrates in Yarrowia lipolytica
September 2026
The efficient use of hydrophobic substrates such as n-alkanes and fatty acids as carbon and energy sources is an important native ability of Yarrowia lipolytica. This review summarizes the crucial characteristics...
P450 engineering via structure-guided rational design achieves high C21-selectivity and bioconversion in steroid biosynthesis
September 2026
C21-hydroxylation is a crucial step in the synthesis of corticosteroids. For instance, C21-hydroxylase catalyzes the conversion of progesterone (PRO) into 11-deoxycorticosterone (DOC, the precursor...
High-yield production of hydroxytyrosol through metabolic engineering of Yarrowia lipolytica
September 2026
Hydroxytyrosol (HT) is a natural antioxidant widely employed in the food production, pharmaceutical, and cosmetic industries. Microbial fermentation for HT production holds significant potential for...
De novo biosynthesis of putrescine from glucose using engineered Escherichia coli in a two-step process
September 2026
Putrescine, is a key monomer for the synthesis of high-performance polymer materials. However, its sustainable microbial production remains challenging in yield and efficiency. In this study, we developed...
Engineered eMutS based magnetic separation system for high-fidelity DNA synthesis
September 2026
DNA synthesis fidelity remains a critical bottleneck in large-scale gene assembly. Although conventional post-synthetic purification methods (e.g., HPLC, PAGE) and enzymatic mismatch-cleavage strategies...
Structure-coevolution dual-guided engineering of C-glycosyltransferase enables high-level biosynthesis of vitexin in Yarrowia lipolytica
September 2026
Flavonoid C-glycosides are bioactive compounds with significant pharmaceutical potential. While biosynthesis offers a sustainable and green alternative to traditional chemical synthesis, its industrial...
Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach
September 2026
Peptide linkers are critical modulators of function in fusion proteins, a foundational technology in modern bioengineering. However, the rational customization of linkers for specific applications remains...
Yeast species resource exploration and application in cell factories
September 2026
Yeasts serve as important industrial hosts, contributing significantly to sustainable and green biomanufacturing efforts in the pursuit of global carbon neutrality, alongside other vital microorganisms....
XRE-type transcriptional regulator ProR controls prodigiosin synthesis in Serratia marcescens JNB5-1
September 2026
The gram-negative bacterium Serratia marcescens produces various secondary metabolites, including prodigiosin, althiomycin, and serratamolide. Among these, prodigiosin exhibits multiple biological activities...
Enhancing cytidine biosynthesis by coupling minicell-forming morphology with biofilm-based fermentation
September 2026
Cytidine is an important nucleoside in the pharmaceutical and biotechnology fields. To improve its biosynthesis efficiency, this study proposes a strategy that integrates minicell-forming recombinant...
Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus
September 2026
Large amounts of xylose mother liquor (XML) are produced as a byproduct of the xylose industry and are often treated as waste. Salidroside is a high-value glycoside with pharmaceutical and nutraceutical...
Heterologous expression of hagfish thread proteins EsTKα and EsTKγ in Pichia pastoris and in vitro self-assembly into an ordered fibrous network
September 2026
Eptatretus stoutii thread keratin α and γ (EsTKα and EsTKγ) are essential structural components conferring hagfish slime with exceptional strength and toughness. These proteins exhibit exceptional mechanical...
Advances in detecting category A biothreat agents: Toward symptom-driven sensing strategies
September 2026
The rise of emerging technologies like synthetic biology and artificial intelligence has raised alarms about new forms of weaponized diseases, given their threat to life and societal economic burden....
Advances in vehicles for in situ delivery: From classical vectors to biologically inspired structures
September 2026
The emerging fields of synthetic biology and gene therapy rely on delivery systems to introduce the nucleic acids and proteins into recipient cells. Hence, the development of delivery tools with high...
Engineered Aspergillus niger enables high-yield heterologous kojic acid production
September 2026
Industrial-scale production of kojic acid (KA)—valued in cosmetics, food, and pharma—has been hindered by limited metabolic engineering strategies for native producers (e.g., Aspergillus oryzae), which...
Signal peptide engineering of a novel M4 family keratinase and the action mechanism in promoting pepper tolerance to salt stress through KerJY23-hydrolyzed feather waste
September 2026
Microbial keratinase has attracted significant attention for its ability to convert keratin waste into high-value products with substantial application potential in the agricultural field. However,...
Time-resolved transcriptomics reveals mechanisms of acute and sustained low-pH adaptation in Corynebacterium glutamicum
September 2026
Corynebacterium glutamicum is a major industrial cell factory for amino acid production. Acidic byproduct accumulation can lower broth pH, disrupt cytoplasmic pH homeostasis, induce oxidative stress,...
Systematic mining and engineering of signal peptides to achieve efficient secretion of cephalosporin C acylase in Escherichia coli
September 2026
Cephalosporin C acylases (CCAs) catalyze the hydrolysis of cephalosporin C to 7-aminocephalosporanic acid, a key intermediate for semisynthetic cephalosporin antibiotics. The functional secretion of...
Semi-rational engineering of terminal deoxynucleotidyl transferase for high-efficiency enzymatic de novo DNA synthesis
September 2026
The 21st century has witnessed rapid advancements in synthetic biology, with DNA synthesis emerging as a foundational technology. Conventional phosphoramidite-based methods face significant limitations,...
S267P mutation of OxyR regulator in Zymomonas mobilis: mechanism of oxidative stress tolerance and applications in cellulosic hydrolysate fermentation and oxidative stress monitoring
September 2026
The peroxide-sensing transcriptional regulator OxyR plays a pivotal role in bacterial oxidative stress responses. The OxyRS267P mutation was previously identified in Zymomonas mobilis ZMNP, a mutant...
Development of mycelium-reinforced dialdehyde cellulose–polyvinyl alcohol composites for emerging high-performance sustainable materials
September 2026
Dialdehyde cellulose (DAC), featuring reactive carbonyl groups introduced via oxidation of hemp-derived cellulose fibers (confirmed by FTIR spectroscopy), was synthesized to promote strong hydrogen...
Formate-driven efficient pyruvate biosynthesis in Vibrio natriegens
September 2026
Formic acid (FA), a key one-carbon liquid compound derived directly from CO2, can serve as a dual-purpose substrate in microbial metabolism, supplying both carbon and energy. Its potential for green...