Construction of a library of gene expression elements in industrial Streptomyces and its application
September 2026
Streptomycetes are key producers of natural products (NPs), and engineering these organisms represents an effective strategy for enhancing NPs production. However, genetic elements (e.g., promoters...
Utilization, metabolic regulation and applications of hydrophobic substrates in Yarrowia lipolytica
September 2026
The efficient use of hydrophobic substrates such as n-alkanes and fatty acids as carbon and energy sources is an important native ability of Yarrowia lipolytica. This review summarizes the crucial characteristics...
P450 engineering via structure-guided rational design achieves high C21-selectivity and bioconversion in steroid biosynthesis
September 2026
C21-hydroxylation is a crucial step in the synthesis of corticosteroids. For instance, C21-hydroxylase catalyzes the conversion of progesterone (PRO) into 11-deoxycorticosterone (DOC, the precursor...
High-yield production of hydroxytyrosol through metabolic engineering of Yarrowia lipolytica
September 2026
Hydroxytyrosol (HT) is a natural antioxidant widely employed in the food production, pharmaceutical, and cosmetic industries. Microbial fermentation for HT production holds significant potential for...
Precise l-threonine-to-l-isoleucine pathway regulation for engineering high-efficiency whole-cell biocatalysts
June 2026
l-Isoleucine (L-Ile), a critical branched-chain amino acid with diverse applications in food, pharmaceutical, and cosmetic industries, is difficult to produce efficiently at scale in microbial systems...
Combinatorial engineering of enzyme and pathway for efficient β-farnesene bioproduction in Yarrowia lipolytica
June 2026
β-farnesene, a natural sesquiterpene compound, has gained significant attention due to its versatile applications in agriculture, industry, biofuels, and related fields. Microbial biosynthesis offers...
Multi-omics elucidation of resource allocation for enhanced ethanol production via precise glucose control in anaerobic Saccharomyces cerevisiae fermentation
June 2026
Ethanol, a high-demand clean energy source, is primarily produced via fed-batch fermentation in industrial settings. Although our previous study identified an optimal glucose concentration of 30 g/L...
High-level production of vitamin K2 in Escherichia coli via modular molecular engineering
June 2026
Menaquinone-7 (MK-7), a key form of vitamin K2 with wide-ranging nutritional and pharmaceutical applications, has attracted increasing interest for microbial production. Here, we developed an integrated...
Synthesis of tunable copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate by engineered Halomonas bluephagenesis and their characterizations
June 2026
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with a 0–30 mol% controllable range of 3HV ratios was produced by Halomonas bluephagenesis (H. bluephagenesis) and characterized. An endogenous plasmid...
Programmable large-cargo integration: Overcoming size constraints for next-generation gene therapy
June 2026
The emergence of base and prime editors—genome editing tools that avoid double-strand breaks (DSBs)—has enabled precise point mutations, insertions, inversions, deletions, and substitutions, which accelerates...
Functions and optimization of soft law in the international governance of synthetic biology: The predicament of hard law vs. the rise of soft law
June 2026
Synthetic biology, as an emerging field that integrates life sciences and engineering technology, is driving profound transformations in global science, ethics, and legal systems. In international legal...
Engineering an ATP-saving mevalonate pathway for high-efficiency S-(+)-linalool production in Serratiamarcescens
June 2026
(S)-(+)-linalool, a valuable acyclic monoterpenol secondary metabolite of plants, finds extensive applications in the food, flavor and fragrance, pharmaceutical, and daily chemical industries. Microbial...
Transfer learning with pre-trained language models for protein expression level prediction in Escherichia coli
June 2026
Accurately predicting recombinant protein expression in Escherichia coli remains a long-standing challenge due to the multifactorial nature of gene regulation and translation. Existing computational...
Enhancing delta-tocotrienol production in Saccharomyces cerevisiae via metabolic engineering strategies in conjunction with the mutagenesis of tocopherol cyclase
June 2026
Delta (δ)-tocotrienol is a member of the vitamin E family and exhibits bioactivities such as antioxidant, anti-inflammatory, and neuroprotective activities. As a nutrient with protective effects on...
Formatotrophic Komagataella phaffii expressing recombinant xylanase via metabolic engineering
June 2026
Formate utilization as a sustainable carbon source for microbial production of high-value chemicals and heterologous proteins presents considerable safety and environmental benefits over conventional...
Metabolic engineering enables Escherichia coli to grow on 1,3-propanediol
June 2026
1,3-propanediol (1,3-PDO) is used to synthesize plastics used in many consumer products. As the demand and production of such plastics increase, a technology will be needed to utilize 1,3-PDO released...
SPIN-guided engineering of a novel (R)-amine transaminase from Fusarium albosuccineum for enantioselective synthesis of chiral piperidyl amines
June 2026
Aminotransferases are promising green biocatalysts for the synthesis of chiral amines, yet their limited catalytic efficiencies restrict broader industrial applications. In this study, a novel (R)-amine...
Design principles for optogenetic-based targeted protein degradation
June 2026
Precise regulation of protein abundance is essential for understanding dynamic cellular processes and for advancing therapeutic development. However, existing approaches lack the spatiotemporal resolution...
Regulating protein glycosylation modification enhances the synthesis of taxadiene in Saccharomyces cerevisiae
June 2026
Utilizing synthetic biology techniques to construct recombinant engineered cells for the synthesis of paclitaxel and its key precursors has emerged as an effective method to address the supply–demand...
Development of a suite of activatable plant synthetic promoter systems using a bacterial LysR-type transcriptional regulator FdeR
June 2026
Advancing plant synthetic biology requires an abundant supply of orthogonal and tunable genetic parts to express multiple genes simultaneously. Current genetic parts, particularly promoters, used in...
RECC: A Red/ET–CRISPR/Cas9-based system enabling genome mining of marine Pseudoalteromonas for novel natural products
June 2026
Marine microorganisms possess vast biosynthetic potential, yet most of their biosynthetic gene clusters (BGCs) remain transcriptionally silent under laboratory conditions. Genetic intractability has...
Modifying the upstream open reading frames of cellulase gene enhances cellulase production in Penicillium oxalicum
June 2026
Cellulase plays an irreplaceable role in biomanufacturing using plant biomass as feedstock. However, improving cellulase production by fungi through manipulation of upstream open reading frames (uORFs)...
Engineering of plant-derived P450scc for de novo biosynthesis of pregnenolone
June 2026
In contrast to the extensively researched animal CYP11A1 system, the catalytic mechanism of sterol side-chain cleavage by plant-derived cytochrome P450scc enzymes remains poorly understood. Through...