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ISSN: 2405-805X
CN: 10-1776/Q
p-ISSN: 2097-1206

Enhanced l-serine synthesis in Corynebacterium glutamicum by exporter engineering and Bayesian optimization of the medium composition

l-serine is a versatile, high value-added amino acid, widely used in food, medicine and cosmetics. However, the low titer of l-serine has limited its industrial production. In this study, a cell factory...

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Nonglycosidic C–O bond formation catalyzed by a bifunctional pseudoglycosyltransferase ValL

The C7N antibiotic validamycin A is an antifungal agent widely used as a crop protectant. It comprises a validoxylamine A unit linked to a glucose moiety, which is formed through a nonglycosidic C–N...

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Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production

Xylose is a major component of lignocellulose, and it is crucial to enable microorganisms the capability of efficient xylose utilization for economical lignocellulosic biochemical production. Zymomonas...

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T7 RNA polymerase-guided base editor for accelerated continuous evolution in Bacillus subtilis

Targeted in vivo hypermutation mediated by base deaminase-T7 RNA polymerase (T7 RNAP) fusions promotes genetic diversification and accelerates continuous directed evolution. Due to the lack of a T7RNAP...

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dTSR enables efficient improvement of heterologous production of spinosad in Saccharopolysporaerythraea

Spinosad (spinosyns A and D) is a highly effective and environmentally friendly insecticide widely used for pest control. However, the difficulty of genetic manipulation in the original strain, Saccharopolyspora...

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Ty retrotransposon element based multiple integration toolkit for Saccharomyces cerevisiae

Extra-high-level overexpression of single or multiple specific proteins by integrating specific genes in the genome is vital to achieve the stable and efficient production of target proteins and metabolites...

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Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution

l-carnitine is a quaternary amine compound essential for eukaryotic metabolism. It is mainly involved in the oxidative decomposition of medium-and long-chain fatty acids and provides energy for the...

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Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases

Recent advancements in data technology offer immense opportunities for the discovery and development of new enzymes for the green synthesis of chemicals. Current protein databases predominantly prioritize...

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Promoter engineering for enhanced 3-hydroxypropionic acid production in Pichia pastoris

Enabling tools are essential for facilitating the methanol bioconversion in Pichia pastoris. However, there is still a relative lack of promoters that can stably express high levels without being affected...

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DNA-CTMF: Reconstruct high quality image from lossy DNA storage via Pixel-Base codebook and median filter

Limited by uncertain base errors in DNA storage, additional correction measures may introduce redundancy or even expand errors, resulting in poor reconstructed image. DNA-CTMF is proposed to reconstruct...

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CgAS, a gene encoding anthranilic acid synthase, contributes to tryptophan biosynthesis and enhanced chaetoglobosin A production in Chaetomium globosum W7

Chaetoglobosin A (cheA) is a complex indole alkaloid exhibiting preferential cytotoxicity against plant pathogens, parasites, and tumor cells. However, the limited production and high synthesis costs...

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Engineering the cytochrome P450 to enhance parthenolide production in Saccharomyces cerevisiae

Parthenolide is confirmed to be an important component of the anticancer drug—ACT001. However, parthenolide biosynthesis in Saccharomyces cerevisiae (S. cerevisiae) was greatly hindered by the low conversion...

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High-yield production and characterization of functional Kir2.1 ion channel using E. coli cell-free protein synthesis system

A family of inwardly-rectifying potassium (Kir) channels plays a key role in the regulation of cellular potassium (K+) balance, affecting muscle, nerve and immune function. Kir channels are comprised...

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Network of nicotinamide mononucleotide biosynthesis: substrate tracking, rate-limiting enzyme, and regulatory strategy

Nicotinamide mononucleotide (NMN) is a nucleotide of significant biological importance, found abundantly in various foods such as meat, fruits, and vegetables. Recently, its potential effects in delaying...

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Biosynthesis of lanosterol in Escherichia coli

Lipid composition represents a significant differentiator across the three domains (eukaryotes, bacteria, and archaea) of cellular life. Eukaryotes possess distinct lipids, such as sterols and sphingolipids,...

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Identification, characterization, and application of an NADPH-dependent carbonyl reductase in Saccharomyces cerevisiae AS2.346 for improving production of (13R,17S)-ethyl secol

Levonorgestrel is currently the most widely used contraceptive, with advantages that include rapid oral absorption and high bioavailability. Among the various synthetic methods employed in its production,...

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Highly efficient synthesis of the natural product β-himachalene in yeast and its potential as a novel anti-HSV-1 therapeutic agent

Terpenoids, a high-value natural product family in nature, have garnered significant attention due to their diverse pharmaceutical activities. However, the current supply of most structurally complex...

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Robust production of N-acetyl-glucosamine in engineered Escherichia coli from glycerol-glucose mixture

Achieving a balanced cell growth and biosynthesis of target products is a pivotal challenge confronted in the realm of green biomanufacturing of chemicals, and it holds the promise of significantly...

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Metabolic reprogramming and computation-aided protein engineering for high-level de novo biosynthesis for 2-phenylethanol in Pichia pastoris

2-Phenylethanol (2-PE), an aromatic compound with a characteristic rose fragrance, is extensively used in the food and cosmetic industries as a flavoring and fragrance agent. Due to limitations in obtaining...

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From reactants to products: computational methods for biosynthetic pathway design

One of the main goals in synthetic biology is to produce value-added compounds from available precursors using enzymatic approaches. The construction of biosynthetic pathways for synthesizing target...

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Construction of a synthetic biology toolkit for the genetic manipulation in the cellulose-producing strain Kosakoniaoryzendophytica

Bacterial cellulose (BC), a robust and highly crystalline nanomaterial composed of glucose polymers, exhibits exceptional properties including superior water retention capacity, biocompatibility, and...

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Morphology-engineered alleviation of mycelial aggregation in Streptomyces chassis for potentiated production of secondary metabolites

The genus Streptomyces exhibits a complex life cycle of morphological differentiation and an extraordinary capacity to produce numerous bioactive secondary metabolites. In submerged cultures, Streptomyces...

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Construction of a genome-engineered stable 5-aminolevulinic acid producing Corynebacterium glutamicum by increasing succinyl-CoA supply

5-Aminolevulinic acid (5-ALA), a versatile precursor for tetrapyrrole derivatives (such as heme, chlorophyll, and cobalamin), drives advancing microbial cell factories to meet growing biomedical and...

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Enzyme and cofactor engineering to increase D-xylonate dehydratase activity for improved D-1,2,4-butanetriol production from D-xylose

D-1,2,4-butanetriol (BTO), a C4 platform compound, is widely used in fields such as military and pharmaceuticals. Biosynthesis of D-1,2,4-BTO from lignocellulose-derived D-xylose presents a promising...

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A multifunctional switch for label-free CRISPR/Cas12a sensor with self-driven amplification

MicroRNA (miRNA) is promising candidate for non-invasive diagnostic biomarker. Conventional CRISPR/Cas12a-based miRNA detection systems are constrained by reliance on reverse transcription, nucleic...

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