Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system
June 2026
Compared with conventional microbial hosts, filamentous fungi have distinct advantages for the industrial-scale biosynthesis of high-value chemical compounds. However, current research on strain engineering...
Molecular landscape analysis of azinothricin-type natural products enables the identification of kettapeptin from Streptomyces durmitorensis
June 2026
The azinothricin family of hybrid hexadepsipeptide-polyketide natural products exhibit remarkable bioactivities, including potent antibacterial, antitumor, antimalarial and anti-inflammatory activities....
Development of a suite of activatable plant synthetic promoter systems using a bacterial LysR-type transcriptional regulator FdeR
June 2026
Advancing plant synthetic biology requires an abundant supply of orthogonal and tunable genetic parts to express multiple genes simultaneously. Current genetic parts, particularly promoters, used in...
Biosynthesis of patchoulol via metabolic engineering the oleaginous red yeast Rhodotorula toruloides
June 2026
Patchoulol is a widely used sesquiterpenoid in perfumes, cosmetics, foods and pharmaceuticals. The plant-dependent production is suffering from limited growing area, long seasonal cycle, etc. Microbial...
A growth-coupled progesterone-responsive biosensor for high-throughput microfluidic screening in Saccharomyces cerevisiae
June 2026
Poor aqueous solubility of steroid precursors, such as pregnenolone and progesterone, limits microbial biotransformation and high-throughput strain screening, representing a bottleneck for strain improvement...
Topoisomerase II-Like variants enhance acid tolerance and low-pH l-malic acid biosynthesis in metabolic engineered Aspergillus nidulans
March 2026
l-malic acid is a versatile compound having extensive applications across food, pharmaceutical, medical, and chemical industries while its production from microbial fermentation offers sustainable advantages...
Hfq modulates system-wide regulatory networks controlling biocontrol property in Lysobacter enzymogenes
March 2026
Lysobacter enzymogenes is a beneficial soil bacterium renowned for its potent biocontrol properties, primarily attributed to antimicrobial secondary metabolites such as HSAF and WAP-8294A2, as well...
Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production in Yarrowia lipolytica
March 2026
Succinic acid, an essential platform chemical with extensive utility in biodegradable materials, pharmaceuticals, and the food industry, faces challenges of high energy consumption and environmental...
A near-infrared ratio fluorescent probe achieves mitochondrial hydrogen polysulfide imaging for monitoring ferroptosis in arthritis
March 2026
Reactive sulfur (RSS) is a type of sulfur-containing molecule widely present in biological systems. Hydrogen polysulfide (H2Sn, n > 1), as a member of the active sulfur family, plays an indispensable...
Construction and analysis of a cell factory for terpenoid biosynthesis in Pichia pastoris via metabolic engineering and metabolomics
March 2026
Terpenoids are widely distributed in nature and have various applications in health products, pharmaceuticals, and fragrances. Despite the tremendous potential of terpenoids, traditional production...
A rapid combinatorial assembly method for gene cluster characterisation illuminates glidobactin biosynthesis
March 2026
Heterologous expression of natural product gene clusters in tractable hosts offers great promise for achieving sustainable production of nature-inspired drugs. However, it is common for the roles of...
Efficient trans-aconitic acid production using systematically metabolic engineered Escherichia coli
March 2026
trans-Aconitic acid (TAA) is a versatile platform biochemical exhibiting extensive applications. In this work, Escherichia coli W3110 (DE3) was metabolic modified for the de novo biosynthesis of TAA....
Rapid detection of genotoxicity employing an engineered high-sensitivity and tight SOS-response biosensor in Escherichia coli MG1655
March 2026
Industrial wastewater and pesticide residues contain numerous genotoxic compounds that pose serious health risks by causing DNA damage and potentially triggering carcinogenesis. Conventional analytical...
MicroDFBEST: A dCas12b-derived dual-function base editor with programmable editing characteristics for microbial genetic engineering
March 2026
Base editors (BEs) enable precise genome editing, but their use in microbes remains limited by restricted mutagenesis capabilities and narrow editing windows. Here, we reported MicroDFBEST, a novel...
Whole-cell biosensors with regulatory circuits based on a synthetic dual-input promoter enabling the highly sensitive detection of ultra-trace Cd2+
March 2026
The research on whole-cell biosensors tailored for trace and ultra-trace detection remains limited and the biosensors based on natural bacterial heavy metal resistance mechanisms have common issues...
Development of a CRISPR/Cas12a genome editing toolbox in Kluyveromyces marxianus and its application in succinic acid biosynthesis
March 2026
Kluyveromyces marxianus is a promising thermotolerant yeast for industrial biotechnology, but lacks efficient genome engineering tools. A CRISPR/Cas12a genome editing toolbox for K. marxianus was developed...
Development of a novel transformation system using zhongshengmycin in Aspergillus oryzae and Aspergillus niger
March 2026
Filamentous fungi such as Aspergillus oryzae and Aspergillus niger serve as valuable microbial cell factories with diverse applications. However, genetic manipulation in these fungi is often hindered...
Efficient synthesis of l-DOPA in Escherichia coli via cofactor and enzyme engineering
March 2026
The global incidence of Parkinson's disease continues to rise. Levodopa (l-DOPA) is the core therapeutic drug, and efficient and sustainable production methods are needed. However, the complex metabolic...
A smart gene circuit for precise regulation of tumor cell behavior
March 2026
Personalized gene circuit is a robust mode of cellular regulation that can manipulate intracellular gene expression to achieve desired functional regulation. However, the construction of logic circuits...
Integrated transcriptomic and metabolomic analyses uncover novel genetic targets for enhanced l-tryptophan production in Corynebacterium glutamicum
March 2026
Corynebacterium glutamicum is a promising microbial chassis for the industrial production of l-tryptophan, an essential amino acid with diverse applications and high market value. In our previous work,...
Competitive binding of TeXlnR and TeAmyR regulates cellulase production in Talaromyces endophyticus
March 2026
Efficient cellulase production is essential for its application in green bioprocessing, yet the transcriptional regulation of cellulase biosynthesis in Talaromyces sp. remains largely uncharacterized....
Amplicon-based prediction of secondary metabolic potential of microbiomes facilitates natural product discovery
March 2026
The rapid exploration of natural products not only expands chemical diversity but also plays a vital role in drug discovery by identifying bioactive agents (e.g., antibiotics), revealing novel biological...
A simplified synthetic microbial community enhances resistance of crucian carp (Carassius auratus) to Aeromonas hydrophila infection through host immune activation
March 2026
Bacterial diseases represent a major bottleneck in the sustainable development of aquaculture. The gut microbiota plays a vital role in host growth and health, including the enhancement of disease resistance....
Synergistic engineering PETase reveals loop-region mutations for enhanced catalytic activity and thermal stability
March 2026
Biodegradation of Polyethylene Terephthalate (PET) offer a strategic avenue for addressing the global plastic pollution crisis. However, the degradation performance of PET hydrolases remains a technological...