Genetic engineering of cyanophages for harmful cyanobacterial bloom treatment—A mini review
April 2027
Harmful cyanobacterial blooms (HCBs) are a prominent worldwide environmental concern. Proliferation of HCBs outpaces the development of mitigation and management technologies. Recently, cyanophages...
Artificial gene clusters designed for simultaneous multi-gene expression enable reconstruction of metabolite biosynthesis pathways in Trichoderma reesei
April 2027
The filamentous fungus Trichoderma reesei represents a highly promising chassis cell for the efficient synthesis of both protein and non-protein metabolites. Despite the availability of diverse genetic...
Hijacking host RNA polymerase for processive in situ mutagenesis
April 2027
Targeted mutagenesis is a crucial tool for in vivo continuous evolution. However, existing methods for long-range targeted mutagenesis in vivo often face limitations such as low efficiency, poor host...
A 6His-2Flag dual-tag strategy enables high-yield production of antimicrobial peptide N6 in Pichia pastoris to combat multidrug-resistant Klebsiella pneumonia
April 2027
In response to the global threat posed by multidrug-resistant Klebsiella pneumoniae, this study established an efficient heterologous expression system for the antimicrobial peptide N6 in Pichia pastoris...
Harnessing synthetic microbial communities for desert vegetation restoration
April 2027
The core bottleneck for desert vegetation restoration is extreme water stress during early seedling establishment, with hydraulic failure as the primary lethal mechanism. Traditional engineering measures...
Reassigning an amber codon to selenocysteine using a functional cysteine suppression pair in Escherichia coli
March 2027
Selenoproteins, characterized by the presence of selenocysteine (Sec) residues, are widely distributed across all domains of life. The unique attributes of selenoproteins are conferred by Sec, which...
Transfer of chloroplast rbcL and atpB genes to nucleus enables partial rescue of photoautotrophic growth of Chlamydomonas
March 2027
An ancient cyanobacterium has evolved through endosymbiosis to form extant chloroplasts of eukaryotic algae and higher plants. During this process, most genes have been transferred to the nuclear genomes....
System reconstruction of Bacillus licheniformis for efficient expression of alkaline protease
March 2027
Industrial enzymes are widely used in diverse applications, but low productivity limits their further widespread utilization. This research aimed to develop high-performance alkaline protease (AprE)...
Production improvement of dalbavancin precursor A40926B0 in Nonomuraea gerenzanensis through strain reconstruction and fermentation optimization
March 2027
Dalbavancin is a potent next-generation lipoglycopeptide antibiotic, but the biosynthesis of its essential precursor, A40926B0, is severely hindered in its native producer Nonomuraea gerenzanensis....
An orthogonal transcription system enables high-efficiency rescue of measles virus-based vaccine vectors against multiple pathogens
March 2027
Recombinant measles virus (rMeV) vectors are promising platforms for vaccine development against emerging infectious diseases due to their safety, stability, and potent immunogenicity. However, conventional...
Systematic evaluation of the DNA repair machinery facilitated the development of efficient genome editing tools for the red yeast Rhodotorula toruloides
March 2027
The red yeast Rhodotorula toruloides is a robust lipid producer, and its biotechnology applications have been expanded through genetic engineering based on the non-homologous end joining (NHEJ) pathway....
EINN: An enzyme-informed neural network guided by an enzyme-constrained genome-scale metabolic model
March 2027
Predicting cellular metabolism from molecular data is a central challenge in systems biology, with direct implications for engineering microbial cell factories. Genome-scale metabolic models (GEMs)...
Toward engineered microbial chassis for growth–production balancing
March 2027
The inherent conflict between cellular growth and product synthesis, arising from limited resource allocation and metabolic burden, fundamentally constrains the performance of microbial chassis. Traditional...
Metabolic engineering of Escherichia coli for efficient production of d-phenylglycine
March 2027
d-phenylglycine (D-PHG) is a valuable building block extensively used in the synthesis of β-lactam antibiotics and other high-value pharmaceuticals. However, its conventional chemical production relies...
Alternative splicing-induced thioesterase domain deficiency drives the production of natural orsellinic acid derivatives with a pantetheine moiety in nonreducing polyketide synthases
March 2027
Nonreducing polyketide synthase Preu6 catalyzes didepside formation by interaction of starter acyl transferase and thioesterase (TE) domains. Here, we show that an aberrant transcript, preu6β, is generated...
Unlocking raw-corncob consolidated bioprocessing for malic acid production by DyP-enabled lignin deconstruction in Myceliophthora thermophila
March 2027
l-Malic acid is an important C4 platform chemical, but its direct production from raw lignocellulosic biomass by consolidated bioprocessing (CBP) remains constrained by biomass recalcitrance. Here,...
Deep learning-driven de novo design of ribosome binding sites in Paracoccus denitrificans
March 2027
Paracoccus denitrificans is a heterotrophic nitrifying–aerobic denitrifying bacterium widely used in wastewater treatment and as a model system for studying electron transport chains, making it a promising...
Gentamicin and d-serine selective pressure to improve the homogeneity of cells expressing target proteins
March 2027
In this work, selective pressure was applied in E. coli to enrich the population expressing recombinant proteins and suppress the emergence of low-expression phenotypes. To this end, plasmids were constructed...
Systems-level analysis of 1,6-hexanediol stress response and tolerance engineering in Escherichia coli
March 2027
1,6-Hexanediol (1,6-HDO) is an important chemical platform widely used in polymer and pharmaceutical industries, while its toxicity remains a major bottleneck limiting efficient microbial production....
Lactiplantibacillus plantarum as a multifunctional probiotic for inflammatory bowel disease: Mechanisms, synbiotic strategies, and delivery systems
February 2027
Inflammatory bowel disease (IBD) encompasses a group of chronic inflammatory disorders of the bowel, characterized by complex etiologies that are closely associated with an imbalance in intestinal microbiota,...
scYeast: a biological-knowledge-guided foundation model on yeast single-cell transcriptomics
February 2027
Though large-scale pre-trained models are vital for foundational cell modeling, most of them focus on human or mouse systems, with less emphasis on model organisms like yeast (Saccharomyces cerevisiae),...
N-terminal fusion length: The key to reliable and context-preserving regulatory sequence characterization
February 2027
Regulatory sequences are commonly characterized using fluorescent reporters, yet how N-terminal coding context shapes these measurements has not been systematically quantified. Here, we evaluated the...
CodY constrains β-cypermethrin degradation by regulating physiological adaptation and soil ecological performance in Bacillus cereus GW-01
February 2027
β-cypermethrin (β-CY) is a widely used pyrethroid insecticide, yet the regulatory mechanisms that determine microbial degradation efficiency remain unclear. Here, we examined the role of the global...