Structural and functional insights into the iminium ion intermediate in AsKslB-mediated Pictet-Spengler reaction
March 2026
Pictet-Spengler (PS) reactions are pivotal in the biosynthesis of bioactive alkaloids and pharmaceuticals, yet key structural details underlying their enzymatic catalysis remain insufficiently understood....
Integrated transcriptomic and metabolomic analyses uncover novel genetic targets for enhanced l-tryptophan production in Corynebacterium glutamicum
March 2026
Corynebacterium glutamicum is a promising microbial chassis for the industrial production of l-tryptophan, an essential amino acid with diverse applications and high market value. In our previous work,...
An efficient CRISPR-Cas12a tool for iterative genome editing, streamlined minimization, and payload engineering of Pseudomonas phage S1
March 2026
The ability to precisely engineer phage genomes is critical for advancing phage-based biotechnology and therapeutic development. Here, we present a high-efficiency, scarless CRISPR-Cas12a genome editing...
Engineering mixed sugar metabolic channels in Pseudomonas putida to produce vanillic acid
March 2026
Hydrolysis of lignocellulosic biomass produces the mixed sugars of glucose (60–70 %), xylose (20–30 %), and arabinose (2–20 %), etc. Using mixed sugars instead of pure glucose for microbial biosynthesis...
Putative membrane protein Cgl2168 and its variant enhance thermotolerance in Corynebacterium glutamicum
March 2026
Corynebacterium glutamicum ATCC 13032 is widely used as the chassis strain for industrial production of various amino acids, but its thermotolerance limits productivity under high temperatures. Comparative...
CRISPR-based synthetic biology toolkit development in Candida viswanthii and functional analysis of the stress responsive Ena1-like protein
March 2026
Industrial strains from the Candida genus have been applied in production of enzymes, biochemicals, and single-cell protein. However, the synthetic biology manipulation tools for Candida species remain...
Harnessing synthetic biology for tetraterpenoid astaxanthin production: Recent advances and challenges
March 2026
Astaxanthin, a potent lipid-soluble ketocarotenoid, exists in various stereoisomeric, geometric isomeric, and esterified forms. Its unique molecular configuration and biological activity confer significant...
Improving fidaxomicin production through ARTP mutagenesis and fermentation optimization in Actinoplanesdeccanensis
December 2025
Fidaxomicin, a macrolide antibiotic, is widely used to treat Clostridioides difficile infection (CDI). It demonstrats significantly higher clinical efficacy than vancomycin and metronidazole. However,...
Metabolic engineering of Escherichia coli for squalene overproduction
December 2025
Squalene, a lipophilic triterpene with multifaceted bioactivities, faces bioproduction bottlenecks in microbial hosts due to inefficient biosynthetic pathways and limited storage capacity. Here, we...
Recombinant production of amaranthin and other betalain variants with yeast cell factories
December 2025
Betalains are a class of natural pigments found in plants of the Caryophyllales order. Betanin is the dominant betalain on the food colour market, even though over 80 other variants are known. Recombinant...
Engineering T7 RNA polymerase-cascaded systems controlled by nisin and theophylline for protein overexpression and targeted gene mutagenesis in Lactococcus lactis
December 2025
Lactococcus lactis serves as an important platform for heterologous protein production, with the nisin-controlled gene expression (NICE) system being widely employed for regulated protein overexpression....
Genomic and transcriptomic insights into the molecular responses of a biocrust-derived oleaginous microalga Vischeria sp. WL1 to nitrogen depletion and recovery
December 2025
Microalgae surviving in extreme conditions evolve tolerance mechanisms to diverse environmental stresses. Environmental nitrogen limitation or nitrogen starvation poses a significant threat to the growth...
Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium stationis and its application in hypoxanthine biosynthesis
December 2025
Corynebacterium stationis, a high-GC Gram-positive bacterium with significant industrial potential, has faced limitations due to the lack of efficient genetic tools. In this study, we developed a CRISPR/Cas9-based...
Extracellular vesicles enhance the efficacy of ceftiofur against intracellular bacterial infections
December 2025
Bacterial infections pose a major threat to human health and economic stability. In particular, intracellular bacterial infections present significant clinical challenges due to antibiotic resistance...
Highly efficient genome editing in Bacillus subtilis via miniature DNA nucleases IscB
December 2025
Existing CRISPR-based genome editing techniques for Bacillus subtilis (B. subtilis) are limited due to the large size of the cas gene. IscB, a recently reported DNA nuclease, is one-third the size of...
Metabolic engineering Saccharomyces cerevisiae for de novo biosynthesis of hydroxycinnamoyl glycerols
December 2025
Hydroxycinnamoyl glycerols (HCGs) are a class of bioconjugates characterized by the esterification of hydroxycinnamic acids with glycerol. Owing to their diverse biological activities, HCGs are considered...
Decoding metabolic trade-offs in Halomonas elongata: Chemostat-based flux remodeling for industrial ectoine biosynthesis
December 2025
Halomonas elongata, a moderately halophilic γ-proteobacterium of industrial interest, serves as a microbial cell factory for ectoine—a high-value compatible solute extensively utilized in biopharmaceuticals...
Biosynthesis of irregular monoterpene lavandulol in Saccharomyces cerevisiae
December 2025
Lavandulol, the primary chemical constituent of lavender essential oil, is an irregular monoterpene present in Lavandula angustifolia. It has been employed in the spice and cosmetic industries owing...
Machine learning-assisted rational design and evolution of novel signal peptides in Yarrowia lipolytica
December 2025
Microbial proteins hold great promise as sustainable alternatives for future protein sources, and oleaginous yeast Yarrowia lipolytica has emerged as a recognized platform for heterologous protein expression...
Development of a highly efficient p-coumaric acid-responsive biosensor in Saccharomyces cerevisiae
December 2025
Developing biosensors to monitor and regulate intracellular biosynthesis pathways can significantly enhance natural product yields in microbial cell factories. This study created a novel biosensor in...
Decoupled tricarboxylic acid cycle and glycolysis of Corynebacterium glutamicum combined with acetate supply promote high-yield biosynthesis of l-homoserine
December 2025
l-Homoserine is a valuable intermediate with broad applications in the food, pharmaceutical, and chemical industries. Although Corynebacterium glutamicum has been engineered for the efficient biosynthesis...
Engineered membraneless organelles in Corynebacterium glutamicum for enhanced indigoidine biosynthesis and antimicrobial peptide production
December 2025
Liquid-liquid phase separation (LLPS)-driven membraneless organelles (MLOs) have been employed to enhance metabolic efficiency in various microbial cell factories. However, their application in the...
Engineered bacteriophage-based bioimaging Technology: Development and applications
December 2025
Engineered bacteriophages (phages) have emerged as powerful and versatile tools for bioimaging, owing to their natural specificity for bacterial targets and their amenability to functional modification....
Gene Surfing: An efficient and versatile tool for targeted enzyme mining in metagenomics
December 2025
Microbial community studies have established enzymes' pivotal catalytic roles in ecosystem metabolism, yet cultivation-dependent methods fail to exploit uncultured microbial enzyme resources. Metagenomics...