Degradation strategies of pesticide residue: From chemicals to synthetic biology
June 2023
The past 50 years have witnessed a massive expansion in the demand and application of pesticides. However, pesticides are difficult to be completely degraded without intervention hence the pesticide...
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Synthetic biology techniques to tackle heavy metal pollution and poisoning
September 2022
The requirement for natural resources and energy increases continually with the increase in population. An inevitable result of this is soil, water, and air pollution with diverse pollutants, including...
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Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas
September 2023
Industrial expansion has led to environmental pollution by xenobiotic compounds like polycyclic aromatic hydrocarbons and monoaromatic hydrocarbons. Pseudomonas spp. have broad metabolic potential for...
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Improvement of a highly sensitive and specific whole-cell biosensor by adding a positive feedback amplifier
June 2023
In this study, we designed a Cd2+ whole-cell biosensor with both positive and negative feedback cascade amplifiers in Pseudomonas putida KT2440 (LTCM) based on our previous design with only a negative...
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Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies
September 2022
Bacterial communities form biofilms on various surfaces by synthesizing a cohesive and protective extracellular matrix, and these biofilms protect microorganisms against harsh environmental conditions....
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Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest
September 2022
Electroactive microorganisms (EAMs) play important roles in biogeochemical redox processes and have been of great interest in the fields of energy recovery, waste treatment, and environmental remediation....
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DIProT: A deep learning based interactive toolkit for efficient and effective Protein design
June 2024
The protein inverse folding problem, designing amino acid sequences that fold into desired protein structures, is a critical challenge in biological sciences. Despite numerous data-driven and knowledge-driven...
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Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology
September 2023
Microbial bioelectrochemical system (BES) is a promising sustainable technology for the electrical energy recovery and the treatment of recalcitrant and toxic pollutants. In microbial BESs, the conversion...
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Engineering Rubisco to enhance CO2 utilization
March 2024
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a pivotal enzyme that mediates the fixation of CO2. As the most abundant protein on earth, Rubisco has a significant impact on global carbon,...
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Synthetic polycistronic sequences in eukaryotes
December 2021
The need for co-ordinate, high-level, and stable expression of multiple genes is essential for the engineering of biosynthetic circuits and metabolic pathways. This work outlines the functionality and...
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Melatonin biosynthesis pathways in nature and its production in engineered microorganisms
March 2022
Melatonin is a biogenic amine that can be found in plants, animals and microorganism. The metabolic pathway of melatonin is different in various organisms, and biosynthetic endogenous melatonin acts...
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Multivariate modular metabolic engineering and medium optimization for vitamin B12 production by Escherichia coli
September 2024
Vitamin B12 is a complex compound synthesized by microorganisms. The industrial production of vitamin B12 relies on specific microbial fermentation processes. E. coli has been utilized as a host for...
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ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models
September 2024
Genome-scale metabolic models (GEMs) have been widely employed to predict microorganism behaviors. However, GEMs only consider stoichiometric constraints, leading to a linear increase in simulated growth...
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The LysR family transcriptional regulator ORF-L16 regulates spinosad biosynthesis in Saccharopolyspora spinosa
December 2024
Spinosad, a potent broad-spectrum bioinsecticide produced by Saccharopolyspora spinosa, has significant market potential. Despite its effectiveness, the regulatory mechanisms of spinosad biosynthesis...
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Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products
June 2021
The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P. pastoris...
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Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies
June 2022
The global market demand for natural astaxanthin is rapidly increasing owing to its safety, the potential health benefits, and the diverse applications in food and pharmaceutical industries. The major...
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Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica
December 2024
The utilization of industrial biomanufacturing has emerged as a viable and sustainable alternative to fossil-based resources for producing functional chemicals. Moreover, advancements in synthetic biology...
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Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch
September 2024
Strengthening the expression level of integrated genes on the genome is crucial for consistently expressing key enzymes in microbial cell factories for efficient bioproduction in synthetic biology....
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Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesis
September 2023
N-Acetylneuraminic acid (Neu5Ac), the most common type of Sia, generally acts as the terminal sugar in cell surface glycans, glycoconjugates, oligosaccharides, lipo-oligosaccharides, and polysaccharides,...
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Combined strategies for improving the heterologous expression of a novel xylanase from Fusarium oxysporum Fo47 in Pichia pastoris
September 2024
Xylanase, an enzyme capable of hydrolyzing non-starch polysaccharides found in grain structures like wheat, has been found to improve the organizational structure of dough and thus increase its volume....
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Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds
June 2022
Per- and Polyfluorinated alkyl substances (PFAS) are a broad class of synthetic compounds that have fluorine substituted for hydrogen in several or all locations and are globally categorized as PFCs...
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Complex peptide natural products: Biosynthetic principles, challenges and opportunities for pathway engineering
March 2022
Complex peptide natural products exhibit diverse biological functions and a wide range of physico-chemical properties. As a result, many peptides have entered the clinics for various applications. Two...
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SemiSynBio: A new era for neuromorphic computing
September 2024
Neuromorphic computing has the potential to achieve the requirements of the next-generation artificial intelligence (AI) systems, due to its advantages of adaptive learning and parallel computing. Meanwhile,...
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Discovering peptides and computational investigations of a multiepitope vaccine target Mycobacterium tuberculosis
September 2024
Mycobacterium tuberculosis (MTB) is the causative agent of tuberculosis (TB), a prevalent airborne infectious disease. Despite the availability of the Bacille Calmette-Guerin vaccine, its global efficacy...
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Synergetic engineering of Escherichia coli for efficient production of l-tyrosine
December 2023
l-Tyrosine, an aromatic non-essential amino acid, is the raw material for many important chemical products, including levodopa, resveratrol, and hydroxytyrosol. It is widely used in the food, drug,...
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