Engineering Rubisco to enhance CO2 utilization
March 2024
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a pivotal enzyme that mediates the fixation of CO2. As the most abundant protein on earth, Rubisco has a significant impact on global carbon,...
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Synthetic biotechnology for C1 bio-refinery
March 2025
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Integrating chemistry knowledge in large language models via prompt engineering
March 2025
This paper presents a study on the integration of domain-specific knowledge in prompt engineering to enhance the performance of large language models (LLMs) in scientific domains. The proposed domain-knowledge...
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Ergothioneine biosynthesis: The present state and future prospect
March 2025
Ergothioneine (ERG), a rare natural thio-histidine derivative with potent antioxidant properties and diverse biological functions, is widely utilized in food processing, cosmetics, pharmaceuticals,...
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A systematic discussion and comparison of the construction methods of synthetic microbial community
December 2024
Synthetic microbial community has widely concerned in the fields of agriculture, food and environment over the past few years. However, there is little consensus on the method to synthetic microbial...
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Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering
September 2025
Astaxanthin is a natural red carotenoid, commonly used as an additive in the pharmaceutical industry and as a nutritional supplement owing to its notable antioxidant benefits. However, a complex biosynthetic...
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Melatonin biosynthesis pathways in nature and its production in engineered microorganisms
March 2022
Melatonin is a biogenic amine that can be found in plants, animals and microorganism. The metabolic pathway of melatonin is different in various organisms, and biosynthetic endogenous melatonin acts...
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Engineered microorganisms: A new direction in kidney stone prevention and treatment
June 2024
Numerous studies have shown that intestinal and urinary tract flora are closely related to the formation of kidney stones. The removal of probiotics represented by lactic acid bacteria and the colonization...
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Systematic metabolic engineering enables highly efficient production of vitamin A in Saccharomyces cerevisiae
March 2025
Vitamin A is a micronutrient critical for versatile biological functions and has been widely used in the food, cosmetics, pharmaceutical, and nutraceutical industries. Synthetic biology and metabolic...
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Construction of antibiotic-free riboflavin producer in Escherichia coli by metabolic engineering strategies with a plasmid stabilization system
June 2025
Riboflavin, an important vitamin utilized in pharmaceutical products and as a feed additive, is mainly produced by metabolically engineered bacterial fermentation. However, the reliance on antibiotics...
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Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials
September 2021
Bacillus subtilis is a commonly used commercial specie with broad applications in the fields of bioengineering and biotechnology. B. subtilis is capable of producing both biofilms and spores. Biofilms...
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Degradation strategies of pesticide residue: From chemicals to synthetic biology
June 2023
The past 50 years have witnessed a massive expansion in the demand and application of pesticides. However, pesticides are difficult to be completely degraded without intervention hence the pesticide...
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An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilization
June 2025
Yarrowia lipolytica, a safe yeast, efficiently metabolizes lipids for the production of food additives and agricultural products. Boosting its growth and lipid utilization capabilities is crucial to...
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Synthetic polycistronic sequences in eukaryotes
December 2021
The need for co-ordinate, high-level, and stable expression of multiple genes is essential for the engineering of biosynthetic circuits and metabolic pathways. This work outlines the functionality and...
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Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesis
September 2023
N-Acetylneuraminic acid (Neu5Ac), the most common type of Sia, generally acts as the terminal sugar in cell surface glycans, glycoconjugates, oligosaccharides, lipo-oligosaccharides, and polysaccharides,...
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CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris
June 2025
The important methylotrophic yeast Pichia pastoris has been utilized for the production of a variety of heterologous recombinant proteins and has great potential for use in the production of value-added...
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Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies
June 2022
The global market demand for natural astaxanthin is rapidly increasing owing to its safety, the potential health benefits, and the diverse applications in food and pharmaceutical industries. The major...
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Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products
March 2022
UDP-Glycosyltransferases (UGTs) catalyze the transfer of nucleotide-activated sugars to specific acceptors, among which the GT1 family enzymes are well-known for their function in biosynthesis of natural...
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Discovery and modification of cytochrome P450 for plant natural products biosynthesis
September 2020
Cytochrome P450s are widespread in nature and play key roles in the diversification and functional modification of plant natural products. Over the last few years, there has been remarkable progress...
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Recent progress in engineering Clostridium autoethanogenum to synthesize the biochemicals and biocommodities
March 2024
Excessive mining and utilization fossil fuels has led to drastic environmental consequences, which will contribute to global warming and cause further climate change with severe consequences for the...
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Mechanisms and biotechnological applications of transcription factors
December 2023
Transcription factors play an indispensable role in maintaining cellular viability and finely regulating complex internal metabolic networks. These crucial bioactive functions rely on their ability...
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Synergetic engineering of Escherichia coli for efficient production of l-tyrosine
December 2023
l-Tyrosine, an aromatic non-essential amino acid, is the raw material for many important chemical products, including levodopa, resveratrol, and hydroxytyrosol. It is widely used in the food, drug,...
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Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds
June 2022
Per- and Polyfluorinated alkyl substances (PFAS) are a broad class of synthetic compounds that have fluorine substituted for hydrogen in several or all locations and are globally categorized as PFCs...
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High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast
June 2025
Lignocellulose bio-refinery via microbial cell factories for chemical production represents a renewable and sustainable route in response to resource starvation and environmental concerns. However,...
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Complex peptide natural products: Biosynthetic principles, challenges and opportunities for pathway engineering
March 2022
Complex peptide natural products exhibit diverse biological functions and a wide range of physico-chemical properties. As a result, many peptides have entered the clinics for various applications. Two...
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