Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering
September 2025
Astaxanthin is a natural red carotenoid, commonly used as an additive in the pharmaceutical industry and as a nutritional supplement owing to its notable antioxidant benefits. However, a complex biosynthetic...
Engineering Rubisco to enhance CO2 utilization
March 2024
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a pivotal enzyme that mediates the fixation of CO2. As the most abundant protein on earth, Rubisco has a significant impact on global carbon,...
Advances in engineering and applications of synthetic phase-separated membraneless organelles in biotechnology
September 2026
Membraneless organelles (MLOs) formed through liquid-liquid phase separation (LLPS) constitute crucial dynamic microenvironments within cells, capable of selectively concentrating specific molecules...
Multi-gene Co-expression systems in E. coli: From single-vector designs to programmable expression platforms
June 2026
Escherichia coli (E. coli) has long served as a versatile workhorse for recombinant protein production. As synthetic biology expands the demand for coordinated expression of multiple genes, co-expression...
DNA storage: The future direction for medical cold data storage
June 2025
DNA storage, characterized by its durability, data density, and cost-effectiveness, is a promising solution for managing the increasing data volumes in healthcare....
Engineering Yarrowia lipolytica for nervonic acid production by organelle compartmentalization and dynamic promoter coordination
June 2026
Nervonic acid (NA) is of great significance in repairing damaged nerve fibers and promoting the regeneration of neural cells. This study aimed to construct a Yarrowia lipolytica strain capable of producing...
High-yield production of hydroxytyrosol through metabolic engineering of Yarrowia lipolytica
September 2026
Hydroxytyrosol (HT) is a natural antioxidant widely employed in the food production, pharmaceutical, and cosmetic industries. Microbial fermentation for HT production holds significant potential for...
Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production
June 2025
Corynebacterium glutamicum is a versatile industrial microorganism for producing various amino acids. However, there have been no reports of well-defined C. glutamicum strains capable of hyperproducing...
A systematic discussion and comparison of the construction methods of synthetic microbial community
December 2024
Synthetic microbial community has widely concerned in the fields of agriculture, food and environment over the past few years. However, there is little consensus on the method to synthetic microbial...
Programmable spatiotemporal control of CRISPR-Cas12a: Engineering precision for next-generation gene editing and diagnostics
June 2026
CRISPR-Cas12a has emerged as a versatile alternative to Cas9, offering distinct advantages, such as recognition of thymine-rich protospacer adjacent motifs, generation of staggered 5′ DNA breaks, simplified...
Engineering genetic elements for microbial protein expression systems: Advances, challenges, applications, and prospects
March 2026
The rising global demand for sustainable protein sources poses critical challenges across food, pharmaceutical, and industrial biotechnology sectors. Microbial expression systems provide scalable and...
Metabolic engineering and adaptive laboratory evolution enhance squalene production in Yarrowia lipolytica
September 2026
Squalene, a multifunctional natural compound with diverse bioactivities, has significant potential in the nutraceutical and health industries. Microbial synthesis using engineered cell factories represents...
Functions and optimization of soft law in the international governance of synthetic biology: The predicament of hard law vs. the rise of soft law
June 2026
Synthetic biology, as an emerging field that integrates life sciences and engineering technology, is driving profound transformations in global science, ethics, and legal systems. In international legal...
Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates
September 2026
Succinate is a biobased platform chemical with wide applications in food, pharmaceuticals, and biodegradable polymers such as polybutylene succinate. Despite advances in microbial fermentation, cost-effective...
Synergistic engineering PETase reveals loop-region mutations for enhanced catalytic activity and thermal stability
March 2026
Biodegradation of Polyethylene Terephthalate (PET) offer a strategic avenue for addressing the global plastic pollution crisis. However, the degradation performance of PET hydrolases remains a technological...
High-level production of vitamin K2 in Escherichia coli via modular molecular engineering
June 2026
Menaquinone-7 (MK-7), a key form of vitamin K2 with wide-ranging nutritional and pharmaceutical applications, has attracted increasing interest for microbial production. Here, we developed an integrated...
Engineering Yarrowia lipolytica for high-level ergosterol production
June 2026
Ergosterol is the key precursor of steroid drug synthesis. In this experiment, we systematically modified the synthesis of ergosterol. Firstly, we identified key rate-limiting enzymes through systematic...
Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach
September 2026
Peptide linkers are critical modulators of function in fusion proteins, a foundational technology in modern bioengineering. However, the rational customization of linkers for specific applications remains...
Ergothioneine biosynthesis: The present state and future prospect
March 2025
Ergothioneine (ERG), a rare natural thio-histidine derivative with potent antioxidant properties and diverse biological functions, is widely utilized in food processing, cosmetics, pharmaceuticals,...
Structure-coevolution dual-guided engineering of C-glycosyltransferase enables high-level biosynthesis of vitexin in Yarrowia lipolytica
September 2026
Flavonoid C-glycosides are bioactive compounds with significant pharmaceutical potential. While biosynthesis offers a sustainable and green alternative to traditional chemical synthesis, its industrial...
Engineering ethanologenic bacterium Zymomonas mobilis for economic cellulosic bioethanol production using agro-industrial waste corncob residue
September 2026
Cellulosic ethanol is an important biofuel derived from lignocellulosic biomass, which has the potential to mitigate environmental pollution and contribute to achieving carbon neutralization for a sustainable...
Melatonin biosynthesis pathways in nature and its production in engineered microorganisms
March 2022
Melatonin is a biogenic amine that can be found in plants, animals and microorganism. The metabolic pathway of melatonin is different in various organisms, and biosynthetic endogenous melatonin acts...
Systematic metabolic engineering of Escherichia coli for high-level production of pseudouridine via pathway optimization and precursor enhancement
December 2026
Pseudouridine, the C5-ribose epimer of uridine with significant biological functions and clinical applications, was efficiently produced through systematic metabolic strategy of Escherichia coli in...
Efficient synthesis of l-DOPA in Escherichia coli via cofactor and enzyme engineering
March 2026
The global incidence of Parkinson's disease continues to rise. Levodopa (l-DOPA) is the core therapeutic drug, and efficient and sustainable production methods are needed. However, the complex metabolic...
Semi-rational engineering of terminal deoxynucleotidyl transferase for high-efficiency enzymatic de novo DNA synthesis
September 2026
The 21st century has witnessed rapid advancements in synthetic biology, with DNA synthesis emerging as a foundational technology. Conventional phosphoramidite-based methods face significant limitations,...
