Deciphering Rickettsia conorii metabolic pathways: A treasure map to therapeutic targets
2025
Indian tick typhus is an infectious disease caused by intracellular gram-negative bacteria Rickettsia conorii (R. conorii). The bacterium is transmitted to humans through bite of infected ticks and...
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Effect of temperature and CO2 concentration on biological nutrient removal from tertiary municipal wastewater using microalgae Chlorella prototheocoides
2025
This study investigates the potential of phototrophic microalgae, specifically Chlorella protothecoides, for biological wastewater treatment, with a focus on the effects of air temperature and CO2 concentration...
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Green synthesis and characterization of iron nanoparticles synthesized from bioflocculant for wastewater treatment: A review
2025
Nanotechnology is a rapidly expanding field with diverse healthcare, agriculture, and industry applications. Central to this discipline is manipulating materials at the nanoscale, particularly nanoparticles...
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Microbial amidases: Characterization, advances and biotechnological applications
2025
The amidases (EC 3.5.1.4) are versatile hydrolase biocatalysts that have been the attention of academia and industries for stereo-selective synthesis and bioremediation. These are categorized based...
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The role of Micro-biome engineering in enhancing Food safety and quality
2025
Microbiome engineering has emerged as a transformative approach to enhancing food safety and quality by strategically modulating microbial communities. This review critically examines state-of-the-art...
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Current biosensing strategies based on in vitro T7 RNA polymerase reaction
2025
Recently, a unique behavior of T7 RNA polymerase has expanded its functionality as a biosensing platform. Various biosensors utilizing T7 RNA polymerase, combined with fluorescent aptamers, electrochemical...
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Integrative analysis of candidate MicroRNAs and gene targets for OSA management using in silico and in-vitro approach
2025
MicroRNAs (miRNAs) have been implicated in the pathogenesis of human diseases including sleep disorders. The aim of this study is to address the involvement of miRNAs (miR-21 and miR-29) in the pathophysiology...
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Applications of silver nanoparticles synthesized from Pichia kudriavzevii bioflocculant isolated from Kombucha tea SCOBY
2025
Studying the utilization of natural products in the biosynthesis of silver nanoparticles (AgNPs) recently appears to be a fascinating area of research within nanotechnology. These nanoparticles exhibit...
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Cell-free protein synthesis platforms for accelerating drug discovery
2025
Cell-free protein synthesis is a platform for streamlined production of macromolecules. Recently, several proteins with pharmaceutical relevance were synthesised and characterised. Off-the-shelf reagents...
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Eco-friendly synthesis and optimization of CuNPs using a non-pathogenic bioflocculant from Kytococcus sedentarius
2025
Nanotechnology is being used to solve a variety of environmental issues, including wastewater treatment. In the present study, a rapid eco-friendly method was applied to biosynthesize and optimize copper...
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Straightforward MALDI-TOF MS based screening approach for selection of recombinant protein-expressing E. coli
2025
Recombinant protein production is a milestone of modern biotechnology, drug development and scientific research. When obtaining recombinant protein producers, differences in expression levels among...
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Agroinfiltration-mediated transient assay for rapid evaluation of constructs in pigeonpea
2025
The process of generating stable transformants is time-consuming, labor-intensive, and genotype-dependent. In contrast, transient gene expression techniques, such as agroinfiltration, offer a rapid...
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Development of a low-cost microfluidic chip for hyaluronidase-free oocyte denudation in mammals
2025
Infertility in mammals is one of the most intricate medical issues requiring non-traditional interventions. In Vitro Fertilization (IVF) is one of the modern medical technologies currently used to treat...
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Modeling and simulation of soft bio-mimetic fingers with a novel soft thumb design for bionic hand applications using ANN
2025
The paper presents a novel design for a soft bio-mimetic finger and soft thumb structure for bionic hand applications. It introduces an anthropomorphic pneumatic flexible finger system using a PneuNets...
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Microbial electrosynthesis meets synthetic biology: Bioproduction from waste feedstocks
2025
Integrating electrochemistry and biology, microbial electrosynthesis (MES) enhances feedstock-to-product conversion by utilizing electroactive microorganisms to harness electrical energy for driving...
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Supercritical CO2 extraction of artemisinin from Artemisia annua plant and the biotechnological production of artemisinin precursors: A dual-focus review
2025
Artemisinin, a vital compound renowned for its potent antimalarial properties, has garnered significant attention due to its therapeutic importance and critical role in combating malaria. The extraction...
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Emergence of β-lactamase-producing Proteus mirabilis in clinical settings: A genotypic investigation of resistance mechanisms and carbapenemase genes blaNDM-1 and blaKPC-2
2025
Proteus mirabilis, an opportunistic pathogen frequently implicated in urinary and wound infections, has shown increasing multidrug resistance, posing significant therapeutic challenges. The study aims...
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Tackling carbapenem-resistant Acinetobacter baumannii (CRAB) and their virulence factors using biosynthesized silver nanoparticles combined with imipenem
2025
Carbapenem-resistant Acinetobacter baumannii is an extremely hazardous microorganism due to its high level of resistance to a wide array of antibiotics, making it a significant public health concern....
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Biogenic Synthesis of Silver Nanoparticles Using Sida cuneifolia Leaf Extract for Enhanced Antibacterial, Cytotoxic, and Anti-biofilm Activities
Available online 28 July 2025
Antimicrobial resistance (AMR) is one of the global threats that needs to be addressed. Nanotechnology represents a promising way to address this issue due to its multifaceted mode of action. This study...
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Organ-on-chip technology: Opportunities and challenges
2024
Organ-on-chip (OOC) technology is an innovative approach that reproduces human organ structures and functions on microfluidic platforms, offering detailed insights into intricate physiological processes....
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Therapeutic advancements in nanomedicine: The multifaceted roles of silver nanoparticles
2024
Nanotechnology has the advantages of enhanced bioactivity, reduced toxicity, target specificity, and sustained release and NPs can penetrate cell membranes. The small size of silver nanoparticles, AgNPs,...
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Understanding virus retention mechanisms on protein a chromatography based on using different wash buffers – Evaluating the possibility for a generic wash buffer toolbox to improve virus clearance capacity
2024
During manufacturing of mammalian-cell derived monoclonal antibodies (mAbs) virus clearance capacity of the downstream process has to be demonstrated. The protein A chromatography step typically achieves...
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A comprehensive study on anaerobic digestion of organic solid waste: A review on configurations, operating parameters, techno-economic analysis and current trends
2024
The excessive discharge and accumulation of solid organic waste into the environment is of severe concern across the globe. Thus, an efficient waste management system is important to mitigate health...
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Antibiotic susceptibility and virulence factors of bacterial species among cancer patients
2024
Antibiotic resistance is one of the most significant challenges of the 20-s century, and the misuse of antibiotics is a driver of antimicrobial resistance. This study aimed to assess the prevalence...
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The potential of copper oxide nanoparticles in nanomedicine: A comprehensive review
2024
Nanotechnology is a modern scientific discipline that uses nanoparticles of metals like copper, silver, gold, platinum, and zinc for various applications. Copper oxide nanoparticles (CuONPs) are effective...
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