Deciphering Rickettsia conorii metabolic pathways: A treasure map to therapeutic targets
2025
Indian tick typhus is an infectious disease caused by intracellular gram-negative bacteria Rickettsia conorii (R. conorii). The bacterium is transmitted to humans through bite of infected ticks and...
Effect of temperature and CO2 concentration on biological nutrient removal from tertiary municipal wastewater using microalgae Chlorella prototheocoides
2025
This study investigates the potential of phototrophic microalgae, specifically Chlorella protothecoides, for biological wastewater treatment, with a focus on the effects of air temperature and CO2 concentration...
Green synthesis and characterization of iron nanoparticles synthesized from bioflocculant for wastewater treatment: A review
2025
Nanotechnology is a rapidly expanding field with diverse healthcare, agriculture, and industry applications. Central to this discipline is manipulating materials at the nanoscale, particularly nanoparticles...
Microbial amidases: Characterization, advances and biotechnological applications
2025
The amidases (EC 3.5.1.4) are versatile hydrolase biocatalysts that have been the attention of academia and industries for stereo-selective synthesis and bioremediation. These are categorized based...
The role of Micro-biome engineering in enhancing Food safety and quality
2025
Microbiome engineering has emerged as a transformative approach to enhancing food safety and quality by strategically modulating microbial communities. This review critically examines state-of-the-art...
Current biosensing strategies based on in vitro T7 RNA polymerase reaction
2025
Recently, a unique behavior of T7 RNA polymerase has expanded its functionality as a biosensing platform. Various biosensors utilizing T7 RNA polymerase, combined with fluorescent aptamers, electrochemical...
Integrative analysis of candidate MicroRNAs and gene targets for OSA management using in silico and in-vitro approach
2025
MicroRNAs (miRNAs) have been implicated in the pathogenesis of human diseases including sleep disorders. The aim of this study is to address the involvement of miRNAs (miR-21 and miR-29) in the pathophysiology...
Applications of silver nanoparticles synthesized from Pichia kudriavzevii bioflocculant isolated from Kombucha tea SCOBY
2025
Studying the utilization of natural products in the biosynthesis of silver nanoparticles (AgNPs) recently appears to be a fascinating area of research within nanotechnology. These nanoparticles exhibit...
Cell-free protein synthesis platforms for accelerating drug discovery
2025
Cell-free protein synthesis is a platform for streamlined production of macromolecules. Recently, several proteins with pharmaceutical relevance were synthesised and characterised. Off-the-shelf reagents...
Eco-friendly synthesis and optimization of CuNPs using a non-pathogenic bioflocculant from Kytococcus sedentarius
2025
Nanotechnology is being used to solve a variety of environmental issues, including wastewater treatment. In the present study, a rapid eco-friendly method was applied to biosynthesize and optimize copper...
Straightforward MALDI-TOF MS based screening approach for selection of recombinant protein-expressing E. coli
2025
Recombinant protein production is a milestone of modern biotechnology, drug development and scientific research. When obtaining recombinant protein producers, differences in expression levels among...
Agroinfiltration-mediated transient assay for rapid evaluation of constructs in pigeonpea
2025
The process of generating stable transformants is time-consuming, labor-intensive, and genotype-dependent. In contrast, transient gene expression techniques, such as agroinfiltration, offer a rapid...
Development of a low-cost microfluidic chip for hyaluronidase-free oocyte denudation in mammals
2025
Infertility in mammals is one of the most intricate medical issues requiring non-traditional interventions. In Vitro Fertilization (IVF) is one of the modern medical technologies currently used to treat...
Modeling and simulation of soft bio-mimetic fingers with a novel soft thumb design for bionic hand applications using ANN
2025
The paper presents a novel design for a soft bio-mimetic finger and soft thumb structure for bionic hand applications. It introduces an anthropomorphic pneumatic flexible finger system using a PneuNets...
Microbial electrosynthesis meets synthetic biology: Bioproduction from waste feedstocks
2025
Integrating electrochemistry and biology, microbial electrosynthesis (MES) enhances feedstock-to-product conversion by utilizing electroactive microorganisms to harness electrical energy for driving...
Supercritical CO2 extraction of artemisinin from Artemisia annua plant and the biotechnological production of artemisinin precursors: A dual-focus review
2025
Artemisinin, a vital compound renowned for its potent antimalarial properties, has garnered significant attention due to its therapeutic importance and critical role in combating malaria. The extraction...
Emergence of β-lactamase-producing Proteus mirabilis in clinical settings: A genotypic investigation of resistance mechanisms and carbapenemase genes blaNDM-1 and blaKPC-2
2025
Proteus mirabilis, an opportunistic pathogen frequently implicated in urinary and wound infections, has shown increasing multidrug resistance, posing significant therapeutic challenges. The study aims...
Tackling carbapenem-resistant Acinetobacter baumannii (CRAB) and their virulence factors using biosynthesized silver nanoparticles combined with imipenem
2025
Carbapenem-resistant Acinetobacter baumannii is an extremely hazardous microorganism due to its high level of resistance to a wide array of antibiotics, making it a significant public health concern....
Biogenic synthesis of silver nanoparticles using Sida cuneifolia leaf extract for enhanced antibacterial, cytotoxic, and anti-biofilm activities
2025
Antimicrobial resistance (AMR) is one of the global threats that needs to be addressed. Nanotechnology represents a promising way to address this issue due to its multifaceted mode of action. This study...
Cell bank system, establishment, and application in the virus research, diagnosis, and biopharmaceutical industries
2025
The use of cells in research and diagnostic studies is important. This is of paramount importance in the biopharmaceutical product industry because cells are one of the most important platforms in the...
CFD study and Experimental Verification of droplet formation characteristics in a flow-focusing microfluidic device
Available online 16 October 2025
This study numerically investigates droplet formation in a three-dimensional flow-focusing microchannel and validates the results with a lithographically fabricated device (error ). The effects of injection...
MicroRNA Alterations in Sperm of Infertile Men: Insights into Oligozoospermia, Asthenozoospermia, and Teratozoospermia
Available online 15 October 2025
Male infertility makes up almost 50% of infertility cases in couples. Contributing factors include urogenital abnormalities, hormone imbalances, and genetic mutations. Current research highlights the...
Circular Economy through Integrated Industrial Ecology: Innovations in Resource Recovery and Process Re-Design
Available online 13 October 2025
Industrial ecology provides systems-thinking for re-designing industrial processes with greater resource efficiency, waste reduction, and circularity. This review discusses future directions in process...
Development of Doubled Haploid Lines in Spring Wheat (Triticum aestivum L.) Through Wheat × Maize Hybridization and Colchicine Application
Available online 13 October 2025
Bangladesh faces a significant deficit in wheat grain production relative to its national demand, largely due to the prolonged duration-typically 10 to 12 years-required to release a new variety through...
Screening and Characterization of Carboxymethyl Cellulase (CMCase) and Filter Paperase from Psychrophilic Fungus Truncatella angustata BPF5
Available online 9 October 2025
This study characterized a novel cellulase-producing fungus, Truncatella angustata BPF5, isolated from Baramula, Jammu and Kashmir. Cellulase was produced in submerged fermentation using carboxymethyl...