Colorimetric detection and quantification of glutathione in human serum using the peroxidase-like activity of MOF-808FA frameworks
September 2025
The measurement of GSH plays a crucial role in the timely diagnosis, treatment, and overall management of many diseases, significantly impacting human health. In this research, MOF-808FA has been employed...
Development and validation of a chiral bioanalytical method for pharmacokinetic evaluation of paroxetine enantiomers: Insights into enantiomeric interconversion
September 2025
This study focused on the development and validation of a chiral Ultra-Fast Liquid Chromatography (UFLC) method for the enantioselective measurement of Paroxetine enantiomers in plasma as per USFDA...
Integrated analysis of liquid chromatography-mass spectrometry and network pharmacology identified active compounds of Paeoniae Radix Alba for myasthenia gravis treatment
September 2025
Paeoniae Radix Alba (PRA), also known as Baishao, has been used for the clinical treatment of a variety of diseases, however, its role in myasthenia gravis (MG) remains unexplored....
Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation in Drug Development: A Comprehensive Perspective
Available online 10 September 2025
Drug development is a core area of modern medicine, and its history reflects the deep integration of multidisciplinary technologies. Current revolutionary breakthroughs in biotechnology and computational...
Development and Validation of an RP-HPLC Method for the Accurate and Selective Determination of Parthenolide in Mice Plasma
Available online 2 September 2025
The objective of the study is to develop a reverse-phase high-performance liquid chromatography (RP-HPLC) method and validate it to determine Parthenolide (PTL) in the mice plasma....
Exploring the potential mechanism of the active components of Rhizoma Coptidis in tendinopathy treatment applying network pharmacology and molecular docking analysis
August 2025
Rhizoma Coptidis (RC) possesses detoxifying and anti-inflammatory properties, yet its therapeutic mechanism in tendinopathy is unclear. This study investigated the protective effects and molecular mechanisms...
Design, synthesis, and therapeutic potential of pyridazinone-based molecules: An updated comprehensive review
August 2025
Drug discovery involves the design, synthesis and pharmacological evaluation of new chemical entities. Heterocyclic compounds are ring structures in which a carbon atom is replaced by heteroatoms such...
A centrifuge-free, high-throughput liquid-liquid extraction phase separation filter column and its application in the detection of dihydrotestosterone in plasma
August 2025
Liquid–liquid extraction (LLE) remains a critical technique in clinical analysis for sample preparation. However, conventional approaches require centrifugation to separate aqueous and organic phases,...
Unveiling Cloperastine Fendizoate Degradation Using MS/MS: A Novel HPLC Method for Simultaneous Analysis with Parabens and Degradant Characterization
Available online 21 August 2025
Environmental considerations are gaining a worthy role in the effective evaluation of analytical approaches. The purpose of this work is useful for developing and validating a novel green HPLC technique...
Method development and validation of orlistat by using RP-HPLC
June 2025
To accurately quantify orlistat, an anti-obesity drug that is a pancreatic lipase inhibitor thus Depending on the assay system, orlistat illustrates studies at intestines receptors by temporarily blocking...
Exploring the therapeutic effect of flavonoids on kidney health
June 2025
Chronic kidney disease (CKD) is a progressive systemic disease that changes the function and structure of the kidneys irreversibly over months or years. The final common pathological manifestation of...
DFT, molecular docking and receptor inhibition analysis of neuromodulatory molecule- dopamine
May 2025
Although dopamine is an essential neurotransmitter involved in many neurological processes, little is known about its structural characteristics and receptor-binding patterns. The advancement of neuropsychiatric...
Sida cordifolia L. fraction ameliorate renal injury, inflammation, apoptosis and gene expression on cisplatin induced acute kidney injury: Network pharmacology, in vivo and in vitro studies
May 2025
Acute kidney injury (AKI) is known to involve oxidative stress, apoptosis and inflammation and becoming global health issue. Sida cordifolia L. is a medicinal plant having numerous pharmacological properties....
Exploring antiproliferative potential, in silico docking, and molecular dynamics of pyrimidine-morpholine hybrid molecules
May 2025
Cancer remains a leading cause of death worldwide, highlighting the urgent need for novel therapeutic agents. Pyrimidine-morpholine hybrid compounds have been synthesized as potential multitarget anticancer...
Formulation and evaluation of apigenin-loaded microsponge gel for effective angioedema therapy
April 2025
The objective of this project was to create a microsponge gel loaded with apigenin to improve medication release to treat angioedema....
In silico exploration of Aloe vera leaf compounds as dual AChE and BChE inhibitors for Alzheimer’s disease therapy
April 2025
Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes are therapeutic targets associated with Alzheimer's disease. In this study, we focused on bioactive compounds from Aloe vera leaves,...
Design and validation of a stability-indicating RP-HPLC technique for quantifying ivacaftor in bulk and commercial formulations
April 2025
Aim of this study is to develop a novel Reverse Phase-High performance liquid chromatography (RP-HPLC) method was design and validated for accurately estimating ivacaftor (IVF) in granule form, including...
Identification of diagnostic biomarkers and therapeutic targets for abdominal aortic aneurysms based on disulfidptosis mechanism
April 2025
Currently, there is a lack of hallmark diagnostic markers for abdominal aortic aneurysms (AAA). Recent evidence has suggested the efficacy of gene signature related to Disulfidptosis (a novel form of...
The role of Salvia miltiorrhiza compounds in hepatocellular carcinoma: a preliminary investigation based on computational analysis and liquid chromatography-tandem mass spectrometry
April 2025
The potential therapeutic effect of Salvia miltiorrhiza on hepatocellular carcinoma (HCC) has been expounded. This study commenced to identify the diagnostic biomarkers and predict the role of Salvia...
Ginsenoside Rg1 alleviates D-galactose-induced hepatic senescence in mice
April 2025
Aging-associated liver injury, driven by oxidative stress and chronic inflammation, poses a significant clinical challenge with limited therapeutic options. Ginsenoside Rg1, a bioactive saponin from...
A systematic and comprehensive review of the properties and synthesis of anticancer heterocyclic natural products
March 2025
Heterocyclic natural products are abundant in variety and have useful bioactivities. Cancer, being one of the most widespread sets of potentially lethal diseases, has attracted a great deal of attention...
Impurity profiling and forced degradation studies of angiotensin (AT1) blockers: A review
March 2025
This review looks at the latest developments in some analytical techniques employed for impurity profiling and forced degradation studies for sartans, or angiotensin (AT1) receptor blockers, which are...
Single-cell data and network pharmacology screen hub genes related to oxidative stress response in myeloid cell of osteoarthritis
March 2025
Oxidative stress response exerts essential roles in the pathogenesis of osteoarthritis (OA), especially the infiltration of myeloid cells (such as Neutrophil). This study set out to reveal the hub genes...
Computational investigation of Plectranthus neochilus essential oil phytochemicals interaction with dipeptidyl peptidase 4: A potential avenue for antidiabetic drug discovery
March 2025
This study explores the antidiabetic potential of essential oil compounds from Plectranthus neochilus through molecular docking and pharmacological analysis targeting dipeptidyl peptidase-4 (DPP-4),...
