Identification and characterization of the Fd+ 16 Da fragment impurity in therapeutic CD47 monoclonal antibodies expressed in CHO-K1 cell lines
November 2025
Monoclonal antibodies (mAbs) are typically produced using mammalian expression systems—with Chinese hamster ovary (CHO) cells being the most widely employed. To guarantee the efficacy, safety, and consistency...
Single-cell RNA sequencing identifies CCR4 as a therapeutic target for sepsis with DB07845 as a potential inhibitor
November 2025
Sepsis is characterized by immune dysregulation and systemic inflammation. This study aimed to identify key genes and potential therapeutic compounds through scRNA-seq analysis....
Chemical characterization, antioxidant effect and xanthine oxidase inhibition of Micromeria fruticosa (L.) druce hexane extract
November 2025
Micromeria fruticosa (L.) Druce, a medicinal plant applied for several diseases, is recognized for its essential oils and bioactive constituents. However, there is a lack of data concerning the phytochemical...
Unveiling cloperastine fendizoate degradation using MS/MS: A novel HPLC method for simultaneous analysis with parabens and degradant characterization
October 2025
Environmental considerations are gaining a worthy role in the effective evaluation of analytical approaches. Cloperastine fendizoate (CLOP), an antitussive with antihistaminic activity, requires for...
An automated sample pretreatment system for liquid–liquid extraction and its application in the analysis of four steroid hormones in human plasma
October 2025
Liquid–liquid extraction (LLE) is commonly employed in sample preparation, but faces limitations in automation due to solvent leakage during pipetting, interference from conductivity-based liquid level...
Impact of sodium carbonate processing on the chemical profile and antimicrobial properties of saussurea lappa root extracts
October 2025
Saussurea lappa (S. lappa), belongs to the family Asteraceae, is widely used in treating various diseases and disorders, and has diverse biological effects. Impure S. lappa may be contaminated with...
Research progress of liquid chromatography-mass spectrometry in therapeutic drug monitoring of antipsychotic drug: A review of the past decade
October 2025
Antipsychotic drugs (AD) are important for managing psychiatric disorders like schizophrenia and bipolar disorder, however, their clinical use is often complicated by narrow therapeutic windows, variable...
Colorimetric detection and quantification of glutathione in human serum using the peroxidase-like activity of MOF-808FA frameworks
September 2025
The measurement of GSH plays a crucial role in the timely diagnosis, treatment, and overall management of many diseases, significantly impacting human health. In this research, MOF-808FA has been employed...
Development and validation of a chiral bioanalytical method for pharmacokinetic evaluation of paroxetine enantiomers: Insights into enantiomeric interconversion
September 2025
This study focused on the development and validation of a chiral Ultra-Fast Liquid Chromatography (UFLC) method for the enantioselective measurement of Paroxetine enantiomers in plasma as per USFDA...
Integrated analysis of liquid chromatography-mass spectrometry and network pharmacology identified active compounds of Paeoniae Radix Alba for myasthenia gravis treatment
September 2025
Paeoniae Radix Alba (PRA), also known as Baishao, has been used for the clinical treatment of a variety of diseases, however, its role in myasthenia gravis (MG) remains unexplored....
Method Development and validation for determination of Parthenolide in mice plasma using reverse phase high performance liquid chromatography
September 2025
For accurate, fast, and selective determination of PTL, a RP-HPLC method was developed and validated. The objective of the study is to develop a reverse-phase high-performance liquid chromatography...
Network pharmacology, molecular docking, and molecular dynamics simulation in drug development: A comprehensive perspective
September 2025
Exploring the potential mechanism of the active components of Rhizoma Coptidis in tendinopathy treatment applying network pharmacology and molecular docking analysis
August 2025
Rhizoma Coptidis (RC) possesses detoxifying and anti-inflammatory properties, yet its therapeutic mechanism in tendinopathy is unclear. This study investigated the protective effects and molecular mechanisms...
Design, synthesis, and therapeutic potential of pyridazinone-based molecules: An updated comprehensive review
August 2025
Drug discovery involves the design, synthesis and pharmacological evaluation of new chemical entities. Heterocyclic compounds are ring structures in which a carbon atom is replaced by heteroatoms such...
A centrifuge-free, high-throughput liquid-liquid extraction phase separation filter column and its application in the detection of dihydrotestosterone in plasma
August 2025
Liquid–liquid extraction (LLE) remains a critical technique in clinical analysis for sample preparation. However, conventional approaches require centrifugation to separate aqueous and organic phases,...
Method development and validation of orlistat by using RP-HPLC
June 2025
To accurately quantify orlistat, an anti-obesity drug that is a pancreatic lipase inhibitor thus Depending on the assay system, orlistat illustrates studies at intestines receptors by temporarily blocking...
Exploring the therapeutic effect of flavonoids on kidney health
June 2025
Chronic kidney disease (CKD) is a progressive systemic disease that changes the function and structure of the kidneys irreversibly over months or years. The final common pathological manifestation of...
DFT, molecular docking and receptor inhibition analysis of neuromodulatory molecule- dopamine
May 2025
Although dopamine is an essential neurotransmitter involved in many neurological processes, little is known about its structural characteristics and receptor-binding patterns. The advancement of neuropsychiatric...
Sida cordifolia L. fraction ameliorate renal injury, inflammation, apoptosis and gene expression on cisplatin induced acute kidney injury: Network pharmacology, in vivo and in vitro studies
May 2025
Acute kidney injury (AKI) is known to involve oxidative stress, apoptosis and inflammation and becoming global health issue. Sida cordifolia L. is a medicinal plant having numerous pharmacological properties....
Exploring antiproliferative potential, in silico docking, and molecular dynamics of pyrimidine-morpholine hybrid molecules
May 2025
Cancer remains a leading cause of death worldwide, highlighting the urgent need for novel therapeutic agents. Pyrimidine-morpholine hybrid compounds have been synthesized as potential multitarget anticancer...
Formulation and evaluation of apigenin-loaded microsponge gel for effective angioedema therapy
April 2025
The objective of this project was to create a microsponge gel loaded with apigenin to improve medication release to treat angioedema....
The role of Salvia miltiorrhiza compounds in hepatocellular carcinoma: a preliminary investigation based on computational analysis and liquid chromatography-tandem mass spectrometry
April 2025
The potential therapeutic effect of Salvia miltiorrhiza on hepatocellular carcinoma (HCC) has been expounded. This study commenced to identify the diagnostic biomarkers and predict the role of Salvia...
Ginsenoside Rg1 alleviates D-galactose-induced hepatic senescence in mice
April 2025
Aging-associated liver injury, driven by oxidative stress and chronic inflammation, poses a significant clinical challenge with limited therapeutic options. Ginsenoside Rg1, a bioactive saponin from...
