Ferulic acid targets AKT1/STAT3/EGFR signaling to induce apoptosis and inhibit metastasis in gastric cancer cells
January 2026
Identification of potential anti-tubercular inhibitors through virtual screening, DFT, and molecular dynamics simulation studies
January 2026
Tuberculosis (TB) continues to pose a significant threat to global health, a problem intensified by the rise of strains of Mycobacterium tuberculosis that resist multiple drugs. Identifying novel inhibitors...
Immunomodulatory and skin-compatible effects of Amaranthus retroflexus volatile compounds: An in-silico network and pathway-based approach
January 2026
This study investigated the phytochemical profile, in silico skin sensitization, cosmetic toxicity, and endocrine-disrupting potential of compounds from Amaranthus retroflexus identified through GC-MS...
Identification of key molecular targets and mechanism of action in acute kidney injury by integrating network pharmacology and molecular docking
January 2026
Acute kidney injury (AKI) is a frequent renal complication in surgical settings. This study aimed to identify key molecular targets for AKI and to elucidate the mechanism of action of drugs that targeted...
A narrative literature review on revolutionizing traditional Chinese medicine: Exploring the potential of spectral imaging technology
January 2026
Spectral imaging facilitates the spatially resolved detection of chemical components within pharmaceutical compounds through established techniques including fluorescence, near-infrared chemical, mid-infrared,...
RP-HPLC method development and validation for the simultaneous estimation of Piceatannol and L-Arginine in pharmaceutical formulations
January 2026
Dental caries results from microbial dysbiosis, making microbiota balance a superior strategy to antibiotics, which can disrupt oral flora and promote resistance. The Piceatannol–L-Arginine combination...
Trifluridine/tipiracil exhibits potent antitumor activity in triple-negative breast cancer by targeting SKP2 and inducing cell cycle arrest and apoptosis
January 2026
Trifluridine/Tipiracil (TFTD) is an innovative oral medication comprising trifluridine and tipiracil. While TFTD has shown efficacy in metastatic colorectal cancer, its therapeutic potential in triple-negative...
Integrating metabolomics, DPPH assay, and literature mining for quality marker prediction and the establishment of a quality control strategy for Ampelopsis grossedentata
January 2026
A member of Vitaceae, Ampelopsis grossedentata (AG) is well-studied for its rich phytochemistry and bioactivities, indicating high medicinal value; yet, a reliable method for comprehensive quality control...
Preparation, in vitro dissolution, and in vivo pharmacokinetic evaluations of pazopanib tablets
Available online 25 December 2025
Pazopanib is an important agent in the era of targeted cancer therapy, particularly for the treatment of advanced renal cell carcinoma and soft tissue sarcoma. In this study, we evaluated the quality...
Implementation of QbD approach in RP-HPLC method optimization and validation for concurrent estimation of Deflazacort and Tamsulosin hydrochloride under ICH Q14 and Q2R2 framework
November 2025
The simultaneous estimation of Tamsulosin Hydrochloride (TAM) and Deflazacort (DEF) in capsule dosage form requires a reliable analytical method. Reverse-phase high-performance liquid chromatography...
Two-dimensional HPLC method for determining linezolid concentrations in plasma and its clinical application in therapeutic drug monitoring
November 2025
The trough concentration (Cmin) of linezolid (LEN) is closely associated with therapeutic efficacy and adverse reactions in patients with complex Gram-positive bacterial infections. However, substantial...
Identification and characterization of the Fd+ 16 Da fragment impurity in therapeutic CD47 monoclonal antibodies expressed in CHO-K1 cell lines
November 2025
Monoclonal antibodies (mAbs) are typically produced using mammalian expression systems—with Chinese hamster ovary (CHO) cells being the most widely employed. To guarantee the efficacy, safety, and consistency...
Single-cell RNA sequencing identifies CCR4 as a therapeutic target for sepsis with DB07845 as a potential inhibitor
November 2025
Sepsis is characterized by immune dysregulation and systemic inflammation. This study aimed to identify key genes and potential therapeutic compounds through scRNA-seq analysis....
Chemical characterization, antioxidant effect and xanthine oxidase inhibition of Micromeria fruticosa (L.) druce hexane extract
November 2025
Micromeria fruticosa (L.) Druce, a medicinal plant applied for several diseases, is recognized for its essential oils and bioactive constituents. However, there is a lack of data concerning the phytochemical...
Unveiling cloperastine fendizoate degradation using MS/MS: A novel HPLC method for simultaneous analysis with parabens and degradant characterization
October 2025
Environmental considerations are gaining a worthy role in the effective evaluation of analytical approaches. Cloperastine fendizoate (CLOP), an antitussive with antihistaminic activity, requires for...
An automated sample pretreatment system for liquid–liquid extraction and its application in the analysis of four steroid hormones in human plasma
October 2025
Liquid–liquid extraction (LLE) is commonly employed in sample preparation, but faces limitations in automation due to solvent leakage during pipetting, interference from conductivity-based liquid level...
Impact of sodium carbonate processing on the chemical profile and antimicrobial properties of saussurea lappa root extracts
October 2025
Saussurea lappa (S. lappa), belongs to the family Asteraceae, is widely used in treating various diseases and disorders, and has diverse biological effects. Impure S. lappa may be contaminated with...
Research progress of liquid chromatography-mass spectrometry in therapeutic drug monitoring of antipsychotic drug: A review of the past decade
October 2025
Antipsychotic drugs (AD) are important for managing psychiatric disorders like schizophrenia and bipolar disorder, however, their clinical use is often complicated by narrow therapeutic windows, variable...
Colorimetric detection and quantification of glutathione in human serum using the peroxidase-like activity of MOF-808FA frameworks
September 2025
The measurement of GSH plays a crucial role in the timely diagnosis, treatment, and overall management of many diseases, significantly impacting human health. In this research, MOF-808FA has been employed...
Development and validation of a chiral bioanalytical method for pharmacokinetic evaluation of paroxetine enantiomers: Insights into enantiomeric interconversion
September 2025
This study focused on the development and validation of a chiral Ultra-Fast Liquid Chromatography (UFLC) method for the enantioselective measurement of Paroxetine enantiomers in plasma as per USFDA...
Integrated analysis of liquid chromatography-mass spectrometry and network pharmacology identified active compounds of Paeoniae Radix Alba for myasthenia gravis treatment
September 2025
Paeoniae Radix Alba (PRA), also known as Baishao, has been used for the clinical treatment of a variety of diseases, however, its role in myasthenia gravis (MG) remains unexplored....
Method Development and validation for determination of Parthenolide in mice plasma using reverse phase high performance liquid chromatography
September 2025
For accurate, fast, and selective determination of PTL, a RP-HPLC method was developed and validated. The objective of the study is to develop a reverse-phase high-performance liquid chromatography...
