Enhancing 3-hydroxypropionic acid production from recombinant Saccharomyces cerevisiae for using rice straw hydrolysate and sugar cane industrial waste as substrate
December 2025
Rice straw and sugar cane industrial waste are a plentiful source of lignocellulosic biomass with a high polysaccharide content, that is hydrolyzed into sugar for microbial growth and their metabolites....
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Improving fidaxomicin production through ARTP mutagenesis and fermentation optimization in Actinoplanesdeccanensis
December 2025
Fidaxomicin, a macrolide antibiotic, is widely used to treat Clostridioides difficile infection (CDI). It demonstrats significantly higher clinical efficacy than vancomycin and metronidazole. However,...
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Transcriptomic studies on the product stress response revealed that YCF1 is a beneficial factor for progesterone production in Yarrowia lipolytica
December 2025
Progesterone is a widely used therapeutic hormone and a common precursor for the synthesis of pharmaceutical steroids in both mammals and plants. It has been successfully produced in heterologous microorganisms,...
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Metabolic engineering of Escherichia coli for squalene overproduction
December 2025
Squalene, a lipophilic triterpene with multifaceted bioactivities, faces bioproduction bottlenecks in microbial hosts due to inefficient biosynthetic pathways and limited storage capacity. Here, we...
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Systematic design and evaluation of artificial CO2 assimilation pathways
December 2025
With the advancement of industry and bio-agriculture, the effective management of CO2 has emerged as a critical challenge for humanity. This study systematically explores multiple CO2 assimilation pathways...
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Recombinant production of amaranthin and other betalain variants with yeast cell factories
December 2025
Betalains are a class of natural pigments found in plants of the Caryophyllales order. Betanin is the dominant betalain on the food colour market, even though over 80 other variants are known. Recombinant...
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Decoding yeast transcriptional regulation via a data-and mechanism-driven distributed large-scale network model
December 2025
The complex transcriptional regulatory relationships among genes influence gene expression levels and play a crucial role in determining cellular phenotypes. In this study, we propose a novel, distributed,...
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Engineering T7 RNA polymerase-cascaded systems controlled by nisin and theophylline for protein overexpression and targeted gene mutagenesis in Lactococcus lactis
December 2025
Lactococcus lactis serves as an important platform for heterologous protein production, with the nisin-controlled gene expression (NICE) system being widely employed for regulated protein overexpression....
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Genomic and transcriptomic insights into the molecular responses of a biocrust-derived oleaginous microalga Vischeria sp. WL1 to nitrogen depletion and recovery
December 2025
Microalgae surviving in extreme conditions evolve tolerance mechanisms to diverse environmental stresses. Environmental nitrogen limitation or nitrogen starvation poses a significant threat to the growth...
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Engineering Yarrowia lipolytica for high-level β-elemene production via combinatorial metabolic strategies
December 2025
β-Elemene, a pharmacologically active sesquiterpene derived from the traditional Chinese medicinal herb Curcuma wenyujin, exhibits broad-spectrum anti-tumor and anti-inflammatory activities. Currently,...
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Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium stationis and its application in hypoxanthine biosynthesis
December 2025
Corynebacterium stationis, a high-GC Gram-positive bacterium with significant industrial potential, has faced limitations due to the lack of efficient genetic tools. In this study, we developed a CRISPR/Cas9-based...
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From consultors to collaborators – An SOP for advancing ethics engagement in science
December 2025
Synthetic Biology (SynBio) is an interdisciplinary field that tackles global challenges in healthcare, sustainability, food production, among others. However, it also presents critical ethical concerns,...
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Extracellular vesicles enhance the efficacy of ceftiofur against intracellular bacterial infections
December 2025
Bacterial infections pose a major threat to human health and economic stability. In particular, intracellular bacterial infections present significant clinical challenges due to antibiotic resistance...
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Highly efficient genome editing in Bacillus subtilis via miniature DNA nucleases IscB
December 2025
Existing CRISPR-based genome editing techniques for Bacillus subtilis (B. subtilis) are limited due to the large size of the cas gene. IscB, a recently reported DNA nuclease, is one-third the size of...
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A multifunctional switch for label-free CRISPR/Cas12a sensor with self-driven amplification
December 2025
MicroRNA (miRNA) is promising candidate for non-invasive diagnostic biomarker. Conventional CRISPR/Cas12a-based miRNA detection systems are constrained by reliance on reverse transcription, nucleic...
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Development of precise genome editing and multi-copy integration tools in Hansenula polymorpha DL-1
December 2025
Hansenula polymorpha DL-1 is a thermotolerant yeast capable of utilizing multiple renewable carbon sources, making it a promising microbial cell factory for sustainable manufacturing. However, advanced...
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Enzyme and cofactor engineering to increase d-xylonate dehydratase activity for improved d-1,2,4-butanetriol production from d-xylose
December 2025
d-1,2,4-Butanetriol (BTO), a C4 platform compound, is widely used in fields such as military and pharmaceuticals. Biosynthesis of d-1,2,4-BTO from lignocellulose-derived d-xylose presents a promising...
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Metabolic engineering Saccharomyces cerevisiae for de novo biosynthesis of hydroxycinnamoyl glycerols
December 2025
Hydroxycinnamoyl glycerols (HCGs) are a class of bioconjugates characterized by the esterification of hydroxycinnamic acids with glycerol. Owing to their diverse biological activities, HCGs are considered...
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Decoding metabolic trade-offs in Halomonas elongata: Chemostat-based flux remodeling for industrial ectoine biosynthesis
December 2025
Halomonas elongata, a moderately halophilic γ-proteobacterium of industrial interest, serves as a microbial cell factory for ectoine—a high-value compatible solute extensively utilized in biopharmaceuticals...
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Biosynthesis of irregular monoterpene lavandulol in Saccharomyces cerevisiae
December 2025
Lavandulol, the primary chemical constituent of lavender essential oil, is an irregular monoterpene present in Lavandula angustifolia. It has been employed in the spice and cosmetic industries owing...
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Machine learning-assisted rational design and evolution of novel signal peptides in Yarrowia lipolytica
December 2025
Microbial proteins hold great promise as sustainable alternatives for future protein sources, and oleaginous yeast Yarrowia lipolytica has emerged as a recognized platform for heterologous protein expression...
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Enhanced chlorogenic acid production from glucose via systematic metabolic engineering of Saccharomyces cerevisiae
September 2025
Chlorogenic acid (CGA) is a valuable phenolic acid with various pharmaceutical functions. In our previous study, de novo synthesis of CGA in Saccharomyces cerevisiae was achieved. However, its yield...
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Combined metabolic and enzymatic engineering for de novo biosynthesis of δ-tocotrienol in Yarrowia lipolytica
September 2025
δ-Tocotrienol, an isomer of vitamin E with anti-inflammatory, neuroprotective and anti-coronary arteriosclerosis properties, is widely used in health care, medicine and other fields. Microbial synthesis...
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Optimized dual-AAV base editor delivery system with enhanced editing efficiency and virion production titer
September 2025
Base editors (BEs) are a promising tool for precise base conversion in human cells and animals, while the adeno-associated virus (AAV) is the major vector for human gene therapy. However, the size of...
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iCASRED, a scarless DNA editing tool in E. coli for high-efficiency engineering of natural product biosynthetic gene clusters
September 2025
Efficient gene cluster editing tools are one of the key techniques for discovering novel compounds encoded by silent natural product (NP) biosynthetic gene clusters (BGCs) in microbial genomes. Currently,...
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