A low-frequency pulsed EPR spectrometer for hyperfine clock transition measurement
November 2026
Low-frequency electron paramagnetic resonance (EPR) spectroscopy proves large sample space and special selectivity to hyperfine spin related transitions in molecules. For instance, the hyperfine clock...
A designed fusion tag improves soluble expression of nanobody in E. coli
November 2026
Nanobodies are small, single-domain antibody fragments derived from camelid heavy-chain antibodies that offer high stability, strong binding affinity, and excellent potential for therapeutic and diagnostic...
Real-time NMR analysis of HAT1-mediated multi-site acetylation of histone H4 under complex in vitro conditions
Available online 12 November 2026
Histone acetylation is a critical epigenetic modification. Monitoring site-specific acetylation kinetics in complex biological environments remains challenging. Here, we presented a label-free, real-time...
Single-shot multi-slice spiral spatiotemporally encoded MRI with deep-learning-based referenceless distortion correction
Available online 7 November 2026
Ultrafast magnetic resonance imaging (MRI) is critical for high-temporal-resolution applications, but conventional echo-planar imaging (EPI) is prone to geometric distortion, signal loss and blurring...
Heptanuclear iron complex as a potential contrast agent for MRI
August 2026
A heptanuclear complex of the composition [Fe(II)(CN)6{Fe(III)](L)}6](SCN)2, where L = dianion N,N-bis(1-hydroxy-2-benzylidene)-1,7-diamino-4-azageptane has been studied as a potential contrast agent...
Production of isotope-labeled nanobody KN035 in Pichia pastoris yields native-like conformation and PD-L1 binding activity comparable to KN035 expressed in mammalian cells
August 2026
Stable isotope-labeled proteins are indispensable for NMR-based structural studies. E. coli is a cost-effective and high-yield expression host, but it often fails to support proper folding and post-translational...
Impact of mutual and environmental dipole-dipole interactions on the relaxation of MQ NMR coherences in two-spin system in course of their excitation and reconversion
August 2026
The relaxation of multiple-quantum (MQ) NMR coherences on the excitation and reconversion periods of MQ NMR experiment was investigated for the spin-pairs coupled by dipole-dipole interaction. The 1H...
NMR spectral simplification with imidazolium ionic liquid-coated silica nanoparticles
August 2026
NMR spectral simplification is a powerful strategy for facilitating the analysis and structural characterization of complex mixtures. In this work, we present a nanoparticle-assisted protocol that employs...
Dimerization-induced conformational transitions of yeast iso-1 cytochrome c
August 2026
Cytochrome c is a multifunctional protein involved in electron transport and apoptosis. However, its conformational landscape is complex and heterogeneous, which has obscured the functional understanding—particularly...
NMR insights into the role of urea in cellulose dissolution: A stoichiometric study of cellobiose in NaOH/urea aqueous solution
August 2026
The mechanism underlying the enhanced dissolution of cellulose in NaOH/urea aqueous solutions has long been a subject of debate. In this study, high-resolution solution-state 1H and 13C DEPTq NMR spectroscopy...
Chemical exchange saturation transfer NMR: An analytical tool and its applications
August 2026
NMR spectroscopy has proven to be a powerful tool for elucidating structural information and studying dynamic processes. Among NMR techniques, chemical exchange saturation transfer (CEST) is particularly...
Fast three-dimensional NMR spectroscopy reconstruction based on Hankel matrix with two-directional sliding windows
August 2026
Nuclear magnetic resonance (NMR) spectroscopy has grown into a formidable tool in biomedical analysis and quantification. However, the lengthy acquisition for high-dimensional NMR spectroscopy hinders...
OFC: Outside Front Cover
August 2026
Truncated enhanced constraint for low-rank plus sparse in cardiac dynamic MRI reconstruction
May 2026
Cardiac dynamic MRI has been widely used in cine and perfusion imaging, in which low-rank and sparse priors play an important role in reconstructing dynamic images with high temporal and spatial resolution...
Single-scan ultra-selective probing on targeted components from overlapped NMR spectra
May 2026
Benefitting from the non-invasive and quantitative detection property, liquid NMR spectroscopy shows broad applications in the identification, separation, and structural determination of chemical substances...
Structural anisotropy in celery stalks by microscopic MRI and polarized light microscopy
May 2026
Structural anisotropy of celery stalks was studied using T2 anisotropy in microscopic MRI (μMRI) and supplemented by quantitative polarized light microscopy (PLM) at optical resolutions. Parenchyma,...
Fragment-based discovery of TopBP1 inhibitors integrated with AI-driven molecular docking
May 2026
DNA topoisomerase IIβ-binding protein 1 (TopBP1) converges on multiple oncogenic pathways, e.g., Rb, p53, and PI3K/Akt, which are hallmarks of most cancer progression. However, the lack of structural...
A random fractional 13C labeling strategy for P. pastoris expressed eukaryotic membrane proteins for solid-state NMR studies
May 2026
Solid-state NMR (SSNMR) has emerged as an important technique for characterizing membrane protein structures. Using sparse 13C labeling of membrane proteins can significantly enhance spectral resolution....
OFC: Outside Front Cover
May 2026
Spin-echo measurements for capillary rheometry at low field
May 2026
A single spin-echo, acquired in the presence of a constant magnetic field gradient, has a phase proportional to the average velocity of the sample and an amplitude determined by the distribution of...
Passive water exchange between multiple sites can explain why apparent exchange rate constants depend on ionic and osmotic conditions in gray matter
May 2026
Porous materials, such as biological tissue, often have heterogeneous microstructures where imbibed fluid experiences distinct environments on short timescales, but can exchange among different environments...
Mean pore diameters of 3D printed micro-capillaries using the matrix pencil method
May 2026
The matrix pencil method (MPM) is an approach for quantitative analysis of the multi-exponential time-domain signals from relaxation and diffusion NMR experiments. In contrast to other signal processing...
FlexCENT: A frequency-flexible CEST imaging network combining frequency offset encoding and three-dimensional U-Net
May 2026
This study proposes a deep learning-based method termed frequency-flexible chemical exchange saturation transfer (CEST) imaging network (FlexCENT), which enables robust CEST quantification across variable...