Enhanced chlorogenic acid production from glucose via systematic metabolic engineering of Saccharomyces cerevisiae
September 2025
Chlorogenic acid (CGA) is a valuable phenolic acid with various pharmaceutical functions. In our previous study, de novo synthesis of CGA in Saccharomyces cerevisiae was achieved. However, its yield...
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Combined metabolic and enzymatic engineering for de novo biosynthesis of δ-tocotrienol in Yarrowia lipolytica
September 2025
δ-Tocotrienol, an isomer of vitamin E with anti-inflammatory, neuroprotective and anti-coronary arteriosclerosis properties, is widely used in health care, medicine and other fields. Microbial synthesis...
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Optimized dual-AAV base editor delivery system with enhanced editing efficiency and virion production titer
September 2025
Base editors (BEs) are a promising tool for precise base conversion in human cells and animals, while the adeno-associated virus (AAV) is the major vector for human gene therapy. However, the size of...
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iCASRED, a scarless DNA editing tool in E. coli for high-efficiency engineering of natural product biosynthetic gene clusters
September 2025
Efficient gene cluster editing tools are one of the key techniques for discovering novel compounds encoded by silent natural product (NP) biosynthetic gene clusters (BGCs) in microbial genomes. Currently,...
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Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering
September 2025
Astaxanthin is a natural red carotenoid, commonly used as an additive in the pharmaceutical industry and as a nutritional supplement owing to its notable antioxidant benefits. However, a complex biosynthetic...
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Identification of two new flavone 4′-O-methyltransferases and their application in de novo biosynthesis of (2S)-hesperetin in Yarrowia lipolytica
September 2025
Methyltransferases are pivotal enzymes in the biosynthesis of methylated flavonoids, including (2S)-hesperetin. However, existing flavonoid 4′-O-methyltransferase (F4′OMT) enzymes typically exhibit...
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PEX3 gene knockout influences recombinant xylanase expression by Komagataella phaffii
September 2025
Komagataella phaffii is a methylotrophic yeast harboring a tightly regulated alcohol oxidase promoter (PAOX1), which is now widely used for recombinant protein production. During PAOX1 expression phase...
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Development of ultrasound-visualized tumor-targeting engineered bacteria for precise tumor therapy
September 2025
In situ imaging diagnosis and precise treatment of deep tumor tissues are hotspots in life sciences and medical research. In recent years, using focused ultrasound to remotely control engineered bacteria...
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MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila
September 2025
Nowadays, biological databases are playing an increasingly critical role in biological research. Myceliophthora thermophila is an excellent thermophilic fungal chassis for industrial enzyme production...
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A new paradigm for the regulation of A40926B0 biosynthesis
September 2025
Dalbavancin is a novel glycopeptide antibiotic with activity against a broad range of Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). A40926B0 is the direct precursor...
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Efficient production of 2′-fucosyllactose in Pichia pastoris through metabolic engineering and constructing an orthogonal energy supply system
September 2025
2′-fucosyllactose (2′-FL) holds significant role in the infants' nutrition. While microbial production of 2′-FL has predominantly utilized Escherichia coli and Saccharomyces cerevisiae, the potential...
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A systematic study of regulating inorganic polyphosphates production in Saccharomyces cerevisiae
September 2025
Inorganic polyphosphate (polyP), a linear polymer of orthophosphate residues, plays critical roles in diverse biological processes spanning blood coagulation, immunomodulation, and post-translational...
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Programming anti-ribozymes to sense trigger RNAs for modulating gene expression in mammalian cells
September 2025
Synthetic RNA-based switches provide distinctive merits in modulating gene expression. Simple and flexible RNA-based switches are crucial for advancing the field of gene regulation, paving the way for...
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Enhanced l-serine synthesis in Corynebacterium glutamicum by exporter engineering and Bayesian optimization of the medium composition
September 2025
l-serine is a versatile, high value-added amino acid, widely used in food, medicine and cosmetics. However, the low titer of l-serine has limited its industrial production. In this study, a cell factory...
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Nonglycosidic C–O bond formation catalyzed by a bifunctional pseudoglycosyltransferase ValL
September 2025
The C7N antibiotic validamycin A is an antifungal agent widely used as a crop protectant. It comprises a validoxylamine A unit linked to a glucose moiety, which is formed through a nonglycosidic C–N...
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Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production
September 2025
Xylose is a major component of lignocellulose, and it is crucial to enable microorganisms the capability of efficient xylose utilization for economical lignocellulosic biochemical production. Zymomonas...
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T7 RNA polymerase-guided base editor for accelerated continuous evolution in Bacillus subtilis
September 2025
Targeted in vivo hypermutation mediated by base deaminase-T7 RNA polymerase (T7 RNAP) fusions promotes genetic diversification and accelerates continuous directed evolution. Due to the lack of a T7RNAP...
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dTSR enables efficient improvement of heterologous production of spinosad in Saccharopolysporaerythraea
September 2025
Spinosad (spinosyns A and D) is a highly effective and environmentally friendly insecticide widely used for pest control. However, the difficulty of genetic manipulation in the original strain, Saccharopolyspora...
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Ty retrotransposon element based multiple integration toolkit for Saccharomyces cerevisiae
September 2025
Extra-high-level overexpression of single or multiple specific proteins by integrating specific genes in the genome is vital to achieve the stable and efficient production of target proteins and metabolites...
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Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution
September 2025
l-carnitine is a quaternary amine compound essential for eukaryotic metabolism. It is mainly involved in the oxidative decomposition of medium-and long-chain fatty acids and provides energy for the...
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Environment signal dependent biocontainment systems for engineered organisms: Leveraging triggered responses and combinatorial systems
June 2025
As synthetic biology advances, the necessity for robust biocontainment strategies for genetically engineered organisms (GEOs) grows increasingly critical to mitigate biosafety risks related to their...
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Enhancing abscisic acid production in Botrytis cinerea through metabolic engineering based on a constitutive promoter library
June 2025
Abscisic acid (ABA) is an important plant growth regulator with broad applications in agriculture, forestry, and other fields. Currently, the industrial production of ABA primarily relies on microbial...
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Proton motive force generated by microbial rhodopsin promotes extracellular electron transfer
June 2025
The primary limitation to the practicability of electroactive microorganisms in bioelectrochemical systems lies in their low extracellular electron transfer (EET) efficiency. The proton motive force...
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Coupling genome-wide continuous perturbation with biosensor screening reveals the potential targets in yeast isopentanol synthesis network
June 2025
The increasing consumption of fossil fuels is contributing to global resource depletion and environmental pollution. Branched-chain higher alcohols, such as isopentanol and isobutanol, have attracted...
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High-efficiency patatin expression strategies in Komagataella phaffii (Pichia pastoris): Expression cassette toolbox and regulation of protein secretion
June 2025
Patatin, a prominent food protein derived from potatoes, is renowned for its exceptional nutritional value. Patatin has been characterized for its diverse physiological attributes, including esterase...
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