Systematic metabolic engineering of Escherichia coli for high-level production of pseudouridine via pathway optimization and precursor enhancement
December 2026
Pseudouridine, the C5-ribose epimer of uridine with significant biological functions and clinical applications, was efficiently produced through systematic metabolic strategy of Escherichia coli in...
Efficient whole-cell biotransformation for gastrodin production via glycosyltransferase engineering and process optimization
December 2026
Gastrodin is a naturally occurring phenolic glycoside derived from Gastrodia elata, which is widely used as an edible medicinal plant in functional foods and dietary supplements in East Asia. Owing...
Two-step bioconversion of pyridoxal 5′-phosphate from pyridoxine through cofactor regeneration and pyridoxine 5′-phosphate oxidase evolution
December 2026
Pyridoxal 5′-phosphate (PLP), a vital cofactor in cellular metabolism, is a high-value compound widely used in the food and pharmaceutical industries. However, the efficient biosynthesis of PLP remains...
iFLinkC-EZ: A scalable and automatable method for the assembly of complex fusion proteins and multi-gene expression constructs based on the iFLinkC framework
December 2026
Standardized methods for the assembly of DNA constructs have become indispensable in synthetic biology, biotechnology and basic research. The majority of frameworks—most prominently based on Golden...
Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
December 2026
Xiamenmycin, a prenylated benzopyran compound produced by Streptomyces xiamenensis 318, exhibits promising anti-fibrotic activity but suffers from low productivity, limiting its pharmaceutical development....
Engineering Kluyveromyces marxianus for 3-hydroxypropionic acid production at elevated temperature from Jerusalem artichoke tubers and crude glycerol
September 2026
This study engineered the thermotolerant yeast Kluyveromyces marxianus to produce 3-hydroxypropionic acid (3-HP), a key precursor for biodegradable plastics, via the malonyl-CoA pathway using non-food...
Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates
September 2026
Succinate is a biobased platform chemical with wide applications in food, pharmaceuticals, and biodegradable polymers such as polybutylene succinate. Despite advances in microbial fermentation, cost-effective...
Exploration of endogenous signal peptides and tandem linkage strategies for enhanced heterologous protein secretion in Lacticaseibacillus paracasei
September 2026
Lacticaseibacillus paracasei (L. paracasei), a probiotic bacterium commonly found in the intestinal tract and fermented products, has been utilized as a tool for generating various bioactive components....
Advances in engineering and applications of synthetic phase-separated membraneless organelles in biotechnology
September 2026
Membraneless organelles (MLOs) formed through liquid-liquid phase separation (LLPS) constitute crucial dynamic microenvironments within cells, capable of selectively concentrating specific molecules...
Sustainable production of glutaric acid in microbial cell factories: Current advances and future prospects
September 2026
Glutaric acid is a significant C5 dicarboxylic acid, extensively utilized in the chemical industry, medicine, and biomaterials. In recent years, the advancement of synthetic biology and metabolic engineering...
A novel LPS-dependent outer membrane-anchoring mechanism for T9SS substrates enables engineered enzyme display and whole-cell PET degradation in Cytophaga hutchinsonii
September 2026
Robust surface display systems are crucial for engineering Gram-negative bacteria as whole-cell biocatalysts. In the efficient cellulose degrader Cytophaga hutchinsonii, cellulases are secreted by the...
Targeted identification of new phaterpenes and elucidation of the relevant biosynthetic pathway in Streptomyces phaeochromogenes OSK-123
September 2026
Sesquiterpenes have highly diverse chemical structures and biological activities, resulting in considerable interest in terms of their application and exploration of new analogs. In this study, we identified...
Metabolic engineering and adaptive laboratory evolution enhance squalene production in Yarrowia lipolytica
September 2026
Squalene, a multifunctional natural compound with diverse bioactivities, has significant potential in the nutraceutical and health industries. Microbial synthesis using engineered cell factories represents...
Metabolic engineering of endogenous MEP pathway for enhanced lycopene production in Escherichia coli
September 2026
Microbial cell factories represent the primary approach for heterologous lycopene synthesis, where gene source selection and pathway regulation have been demonstrated to have a significant impact on...
Reconstructing the hydrogenobyrinic acid synthetic toolkit by combining cell-free systems and metabolic engineering
September 2026
Hydrogenobyrinic acid (HBA) is a pivotal intermediate in the aerobic biosynthesis of cobalamin (vitamin B12), representing a stable, metal-free corrin precursor and a useful substrate for both biological...
A novel TetR-type repressor directly modulates precursor supply and utilization for erythromycin biosynthesis
September 2026
Antibiotic biosynthesis in actinomycetes is controlled by various transcription factors, with TetR family regulators (TFRs) serving as important modulators of this process. We previously discovered...
De novo biosynthesis of eriocitrin in Saccharomyces cerevisiae through deep learning-guided enzyme screening and systematic metabolic engineering
September 2026
Eriocitrin, a flavanone-7-O-disaccharide known for its antioxidant and anti-inflammatory properties, holds considerable promise for use in functional foods and pharmaceuticals. However, its large-scale...
Construction of a globally modular recombinant Saccharomyces cerevisiae chassis for ginkgolide precursor levopimaradiene production
September 2026
The production of biologically active natural products through microbial metabolic factories, such as Saccharomyces cerevisiae, represents a promising approach to achieving green and sustainable production....
Multistep metabolic engineered Klebsiella oxytoca for efficient l-leucine production
September 2026
l-Leucine is widely applied in food, feed and medical industries. In this work, an efficient l-leucine producing strain Klebsiella oxytoca LKO-14 was constructed based on an l-valine producer K. oxytoca...
Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system
September 2026
Bacillus subtilis is widely employed for lignocellulose degradation. However, wild-type strains typically exhibit low and incomplete cellulase activities. This study aimed to engineer recombinant B....
High-throughput editing boosts the construction of next-generation microbial cell factories
September 2026
Traditional metabolic engineering faces numerous challenges in constructing microbial cell factories, such as inefficient gene editing, time-consuming and labor-intensive screening processes, and the...
Enhanced spinosad production in Saccharopolyspora spinosa by employing mannose as an extracellular carbon reservoir and optimizing acetyl-CoA supply pathway
September 2026
Spinosad, a potent broad-spectrum insecticidal polyketide produced by Saccharopolyspora spinosa, faces limitations in industrial-scale production due to inherent inefficiencies in its native biosynthetic...
Metabolic and transport engineering enable efficient de novo biosynthesis of patchoulol in Saccharomyces cerevisiae
September 2026
Patchoulol is a valuable sesquiterpene for fragrance and cosmetics, but plant extraction is slow and resource-intensive. Here, we combined enzyme, pathway and transporter engineering to build a yeast...
Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress
September 2026
Formaldehyde serves as a crucial intermediate metabolite during C1 compounds biotransformation. To optimize C1 utilization efficiency, it is essential to identify genes related to formaldehyde tolerance...
Engineering ethanologenic bacterium Zymomonas mobilis for economic cellulosic bioethanol production using agro-industrial waste corncob residue
September 2026
Cellulosic ethanol is an important biofuel derived from lignocellulosic biomass, which has the potential to mitigate environmental pollution and contribute to achieving carbon neutralization for a sustainable...